Ab182576

Manufactured by Abcam

Sourced in United States

Ab182576 is a lab equipment product offered by Abcam. It is a core component for conducting specific laboratory experiments and analyses. The product's detailed specifications and intended use are not available in this response.

Automatically generated - may contain errors

Lab products found in correlation

Ab32500, by Abcam (3 mentions) Ab18256, by Abcam (2 mentions) Ab76315, by Abcam (2 mentions) Ab74272, by Abcam (1 mentions) M0876, by Agilent Technologies (1 mentions) Ab32512, by Abcam (1 mentions) Rabbit anti akt, by Cell Signaling Technology (1 mentions) Rabbit anti phospho akt ser473, by Cell Signaling Technology (1 mentions) Rabbit anti p44 42 mapk erk1 2, by Cell Signaling Technology (1 mentions) Anti rabbit igg hrp linked antibody, by Cell Signaling Technology (1 mentions) Rabbit anti gapdh, by Cell Signaling Technology (1 mentions) Ecl plus, by Solarbio (1 mentions)

4 protocols using ab182576

Protein Interactions in Cellular Processes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blot and coimmunoprecipitation analyses were conducted as described in our previous research.^{9 (link)} Antibodies against the following proteins were used: gankyrin (ab182576, Abcam), HMGB1 (ab18256, Abcam), STAT3 (ab32500, Abcam), p-STAT3 (ab76315, Abcam), NONO (11058, Proteintech), AR (ab74272, Abcam), and GAPDH (#2118S, CST). To analyze gankyrin, HMGB1 and NONO protein interactions, co-immunoprecipitation assays were used with antibodies against the following: gankyrin (ab182576, Abcam), HMGB1 (ab18256, Abcam), and NONO (11058, Proteintech). The GAPDH was utilized as an internal reference.

Peng G., Wang C., Wang H., Qu M., Dong K., Yu Y., Jiang Y., Gan S, & Gao X. (2023). Gankyrin-mediated interaction between cancer cells and tumor-associated macrophages facilitates prostate cancer progression and androgen deprivation therapy resistance. Oncoimmunology, 12(1), 2173422.

+ Open protocol

+ Expand

Immunohistochemical Profiling of Prostate Cancer

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Paraffin-embedded sections of prostate cancer samples were deparaffinized, rehydrated, and microwaved for 20 min in Tris/EDTA buffer or citric acid buffer to retrieve antigens. Staining was carried out using primary antibodies against the following proteins: Gankyrin (ab182576, Abcam), HMGB1 (ab18256, Abcam), CD68 (M0876, Dako), STAT3 (ab32500, Abcam), p-STAT3 (ab76315, Abcam), and NONO (11058, Proteintech). Samples were scored semiquantitatively according to the percentage of staining intensity and positive cells using the H score (range 0–300), as described previously.^{20 (link)} All the immunohistochemistry assays were repeated three times.

+ Open protocol

+ Expand

Western Blot Analysis of Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

The western blot analysis was performed as described in our previous study^{20 (link)}. The primary antibodies used in the this study were listed as follows: rabbit anti-gankyrin (ab182576), rabbit anti-STAT3 (ab32500), and rabbit anti-STAT3 (Phospho 727) (ab30647), rabbit anti-eotaxin-2 (CCL24) (ab203586), rabbit anti-CCR3 (ab32512) from Abcam (Cambridge, MA, USA) and rabbit anti-GAPDH (#2118S), rabbit anti-p44/42 MAPK (Erk1/2) (#4695), rabbit anti-p44/42 MAPK (Erk1/2) (Phospho Thr202/Tyr204) (#4370), rabbit anti-Akt (#9272), and rabbit anti-Akt (Phospho Ser473) (#9271) from Cell Signaling Technology (Danvers, MA, USA). The secondary antibody used in the assay was anti-rabbit IgG-HRP-linked antibody (#7074S) from Cell Signaling Technology. Coimmunoprecipitation analysis was performed according to previously published protocols^{20 (link)} using the above-mentioned antibodies.

Wang C., Wang Y., Hong T., Cheng B., Gan S., Chen L., Zhang J., Zuo L., Li J, & Cui X. (2020). Blocking the autocrine regulatory loop of Gankyrin/STAT3/CCL24/CCR3 impairs the progression and pazopanib resistance of clear cell renal cell carcinoma. Cell Death & Disease, 11(2), 117.

+ Open protocol

+ Expand

Protein Expression Analysis by Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were lysed by RIPA buffer (Beyond) containing protease inhibitor. Then, protein concentration was evaluated using the BCA method. Protein (25 μg) was separated by 10% SDS-PAGE gel and was transferred to PVDF membrane. Afterward, the membrane was blocked with 5% skim milk and incubated overnight with PSMD10 (ab182576, Abcam), α-tubulin (CST, 3873 s, 1:2000) at 37 °C. Secondary antibody (SA0004–1, Porteintech) was added to the membrane and incubated for 2 h at 25 °C. ECL plus (PE0010, Solarbio) and Tanon-5200 CE (Biotanon, Shanghai, China) were used to observe the protein bands. α-tubulin served as the loading control.

Wang C., Wang B., Liang W., Zhou C., Lin W., Meng Z., Wu W., Wu M., Liao Y., Li X., Zhao J, & He Y. (2022). Hsa-miR-1248 suppressed the proliferation, invasion and migration of colorectal cancer cells via inhibiting PSMD10. BMC Cancer, 22, 922.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!