Mouse anti β amyloid

For Immunofluorescence staining, floating sections were blocked with 5% bovine serum albumin (BSA) and 0.5% Triton-X 100 for 1h at room temperature and then sequentially incubated with primary antibodies overnight at 4°C and the fluorescent-dye-conjugated secondary antibodies for 1h at room temperature. Primary antibodies include: rabbit anti-NG2 (1:500, Millipore, Cat: AB5320), rat anti-MBP (1:200, Millipore, Cat: MAB386), rabbit anti-Iba1(1:500, Wako, Cat: 019–19741), mouse anti-β-Amyloid(1–16) (1:500,Biolegend,Cat: 803007), rabbit anti-Fos (9F6)(1:100, CST, Cat: 2250), goat anti-PDGFRα(1:200, R&D, Cat:AF-1062),rabbit Anti-muscarinic acetylcholine receptor (M1R)(1:500, Sigma, Cat: M9808), mouse anti-caspr(1:100, NeuroMab, Cat: 75–001), rabbit anti-MOG(1:200, Abcam, Cat: ab32760). Appropriate Alexa Fluor-conjugated secondary antibodies include donkey anti-mouse, donkey anti-rabbit, and goat anti-rat (1:1000, Life Technologies). The nuclei were counterstained with DAPI at room temperature. Fluorescent images were captured using a confocal laser-scanning microscope (Olympus, FV 3000, Shinjuku, Tokyo) or a spinning disk confocal super resolution microscope (Olympus, SpinSR10, Shinjuku, Tokyo) or a fluorescence microscope (Zeiss, M2, Germany) with excitation wavelengths appropriate for Alexa Fluor 350(380nm), 488 (488 nm), 594 (568 nm), 647 (628 nm) or DAPI (380 nm).