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Annexin 5 fitc staining

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Annexin V-FITC is a fluorescent dye used to detect and quantify apoptotic cells. It binds to phosphatidylserine, a phospholipid that is translocated to the outer leaflet of the cell membrane during apoptosis. The FITC (fluorescein isothiocyanate) label allows for the detection of bound Annexin V using flow cytometry or fluorescence microscopy.

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Lab products found in correlation

Cellquest software version 3, by BD (1 mentions) Facscan flow cytometer, by BD (1 mentions)

Close spelling variants of this product

Annexin V FITC‐propidium iodide (PI) staining assay Annexin V-FITC/PI staining kit Annexin V/FITC staining kit Annexin-V FITC and PI double staining kit Annexin V-FITC/propidium iodide (PI) staining Annexin V-FITC/PI apoptosis staining kit Annexin V-FITC/PI double staining kit Annexin V-FITC and propodium iodide (PI) staining Kit FITC-labeled Annexin V and Propidium Iodide Staining Solution Annexin V-FITC/PI staining

2 protocols using annexin 5 fitc staining

1

Annexin V-FITC Apoptosis Assay

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Apoptosis was detected using Annexin V-FITC staining (Thermo Fisher Scientific, Inc.). Briefly, a total of 106 ES-2 and SKOV3 cells were washed with PBS and stained with 5 µl of Annexin V and 2 µl of PI for 15 min at 4˚C. Apoptosis cells were detected using a FACScan flow cytometer (BD Biosciences) and the data were analyzed by Cellquest Software version 3.3 (BD Biosciences).
Ablimit T., Tursun G., Zhang Y., Abduxkur G., Abdurexit G, & Abliz G. (2022). Inositol monophosphatase 2 promotes epithelial ovarian cancer cell proliferation and migration by regulating the AKT/mTOR signaling pathway. Experimental and Therapeutic Medicine, 24(5), 668.
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2

Apoptosis Detection by Annexin V-FITC

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Detection of apoptotic cells by Annexin V-FITC staining was performed as recommended by the manufacturer (Thermo Fisher). Briefly, cells were cultured in 24-well plates were washed twice with PBS and stained with Annexin V-FITC for 10 min in the dark. Detection of Annexin V-FITC were performed with a fluorescence microscope after two washing steps with binding buffer.
Hanselmann S., Wolter P., Malkmus J, & Gaubatz S. (2017). The microtubule-associated protein PRC1 is a potential therapeutic target for lung cancer. Oncotarget, 9(4), 4985-4997.
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