Quantum xls ultra ms

Compounds were analysed with a Thermo Fisher Trace 1310 GC coupled to a Thermo Fisher Quantum XLS Ultra MS. Fractions were injected with an autosampler (Thermo TriPlus RSH) onto a fused-silica capillary column (Phenomenex Zebron ZB-5MS, 30 m, 0.1 μm film thickness, inner diameter 0,25 mm). The carrier gas was He at a flow rate of 1.5 mL/min. The GC oven temperature was ramped from 80°C (1 min) to 310°C at 5°C/min and held for 20 min. Electron ionization mass spectra were recorded in full scan mode at an electron energy of 70 eV within a mass range of m/z 50 to 600.
Compounds were identified by their retention times and comparison with published mass spectra. FAME were characterized by comparison of mass spectra and retention times with an external standard mixture (Supelco 37 FAME standard). Compounds were quantified by comparison with internal standards of known concentration.

Gas chromatography-mass spectrometry (GC-MS) analyses of the samples were performed using a Thermo Scientific Trace 1300 Series GC coupled to a Thermo Scientific Quantum XLS Ultra MS. The GC capillary column used was a Phenomenex Zebron ZB–5MS (30 m, 0.1 µm film thickness, inner diameter 0.25 mm). Compounds were transferred splitless to the GC column at an injector temperature of 300 °C. Helium was used as the carrier gas at a flow rate of 1.5 mL/min. The GC temperature program was as follows: 80 °C (hold 1 min), 80 °C to 310 °C at 5 °C/min (hold 20 min). Electron ionization mass spectra were recorded at 70 eV electron energy in full scan mode (mass range m/z 50–600, scan time 0.42 s). Peak areas were integrated using Thermo Xcalibur software version 2.2 (Thermo Fisher Scientific Inc., Waltham, MA, USA).