The IVIS system (Xenogen Corp., Alameda, CA) was used for bioluminescence imaging. Mice were anesthetized with isoflurane and injected intraperitoneally with 200 μL of 7.5 mg/mL luciferin solution (GoldBio, Olivette, MO). Luciferin was allowed to circulate for 10 min post-injection, and mice were placed ventral side up and imaged promptly thereafter. Luminescence signals were quantified using Living Image 4.2 software (Caliper Life Sciences, Hopkinton, MA) with a region of interest (ROI) circling the area over the liver.
Luciferin solution
Manufactured by GoldBio
Sourced in United States
Luciferin solution is a laboratory reagent used in bioluminescence assays. It contains the organic compound luciferin, which is a substrate for the enzyme luciferase. When combined with luciferase and ATP, luciferin emits light through a chemiluminescent reaction.
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Lab products found in correlation
4 protocols using luciferin solution
1
Bioluminescence Imaging of Liver in Mice
2
In vivo Bioluminescence Imaging
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BALB/c or C57BL/6 mice were IV tail-injected with 250 μl of the in vivo-jet PEI delivery reagent (Polyplus transfection, France) complexed with an appropriate luc reporter plasmid. 24 hours later the mice were injected with 250 μl of 3 mg/ml luciferin solution (Gold Biotechnology, USA) and underwent bioluminescence imaging by Biospace Photon Imager (Biospace Lab, France).
3
Tracking NALM6-GL Tumor Progression
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Seven- to 9-week-old, female NSG mice were intravenously injected with 2 × 106 NALM6-GL cells. Three days later, mice were infused with Hu19-CAR T at the doses indicated in the figure legends. Mice received one CAR T cell infusion for all experiments. Bioluminescent images of mice were taken on the day of CAR T cell infusion and every 3 days thereafter. Imaging was done as follows: mice were intraperitoneally injected with 200 μL of 15 mg/mL luciferin solution (GoldBio, Olivette, MO, USA). Bioluminescent images were taken 10 min after luciferin injection, while the mice were under anesthesia with 3% isoflurane. Images were captured using Xenogen IVIS Imaging System with Living Imaging software. Ventral images were captured at 30 s exposures on a 24-cm field of view and at binning factor of 4. Bioluminescence was quantified as the body of the mouse without the tail in units of radiance (p/sec/cm2/sr) using the Living Image software (Xenogen, Alameda, CA, USA). Mice were sacrificed in accordance with the NCI Animal Care and Use Committee guidelines.
4
Luciferase-Expressing MM.1S Cell Xenograft Model
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MM.1 S cells were transduced in our lab with a retrovirus encoding an enhanced firefly luciferase gene60 (link). MM.1S-luciferase cells were stained with a monoclonal antibody against Thy1.1 (BD Biosciences) and sorted by flow cytometry to purity (FACSAria, BD Biosciences). NSG mice from NCI-Frederick or the Jackson Laboratories were injected with 8 × 106 MM.1S-luciferase cells intravenously. After 10 days, CAR T cells at a dose of 1–2 × 106 CD3+CAR+ cells/mouse were infused intravenously. Mice received 1 injection of CAR T cells. For imaging, mice were injected with 100 µL of luciferin solution (15 mg/mL in PBS, GoldBio) and anesthetized with 3% isoflurane. After 10 min, bioluminescence imaging (BLI) was captured using a Xenogen IVIS Imaging System. Ventral images were taken using a 1-min exposure on a 24 cm field of view with a binning factor of 4. BLI was quantified over the body of the mouse without the tail in units of radiance (p/sec/cm2/sr) using the Living Image software (Xenogen). Images were scaled to 107–108 radiance units. Mice were sacrificed upon the onset of severe hind leg paralysis and wasting in accordance with the NCI Animal Care and Use Committee guidelines.
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