Etv4 clone 16

Manufactured by Santa Cruz Biotechnology

ETV4 (clone 16) is a recombinant monoclonal antibody that recognizes the ETV4 protein. ETV4 is a transcription factor that belongs to the ETS family of proteins and plays a role in cellular processes such as cell proliferation, differentiation, and migration.

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Lab products found in correlation

Envision system, by Agilent Technologies (1 mentions) Desmin, by Roche (1 mentions) Ki 67 clone mib 1, by Agilent Technologies (1 mentions) Erg clone epr3864, by Roche (1 mentions) Myogenin clone f5d, by Agilent Technologies (1 mentions) Benchmark ultra, by Roche (1 mentions) Diaminobenzidine, by Agilent Technologies (1 mentions) S100 protein clone poly z311, by Agilent Technologies (1 mentions) Myod1 clone ep212, by Cell Marque (1 mentions) Cd4 4b12, by Leica Biosystems (1 mentions) Desmin clone de r 11, by Roche (1 mentions) Cd30 ber h2, by Agilent Technologies (1 mentions) Benchmark ultra automated stainer, by Roche (1 mentions) Ema clone e29, by Roche (1 mentions)

3 protocols using etv4 clone 16

Immunohistochemical Analysis of WT1 and ETV4

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Tumour tissue samples were fixed in 10% buffered formalin and embedded in paraffin. The 4-μm-thick sections were stained with haematoxylin and eosin. The cells were suspended, embedded in iPGell, fixed with 4% paraformaldehyde in prior to paraffin embedded, and then sections were subjected to heat-induced epitope retrieval. Antibodies against WT1 (6F-H2, 1:50, Dako) and ETV4 (clone 16, dilution 1:50, Santa Cruz Biotechnology) were used. Antigen-antibody reactions were visualized using the EnVision system (Dako).

Oyama R., Takahashi M., Yoshida A., Sakumoto M., Takai Y., Kito F., Shiozawa K., Qiao Z., Arai Y., Shibata T., Araki Y., Endo M., Kawai A, & Kondo T. (2017). Generation of novel patient-derived CIC-DUX4 sarcoma xenografts and cell lines. Scientific Reports, 7, 4712.

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Comprehensive Immunohistochemical Profiling

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Immunohistochemistry was performed on 4 µm sections cut from the paraffin block using an autostainer (Benchmark ULTRA; Ventana Medical System Inc., Tucson, AZ) using the following antibodies: CD99 (clone 12E7, prediluted, Dako ), epithelial membrane antigen (EMA) (clone E29, 1:50; Dako), Ki67 (clone MIB-1, 1: 100; Dako), desmin (clone DE-R-11, prediluted; Roche), myogenin (clone F5D, 1: 2; Dako), smooth muscle actin alpha (clone 1A4, prediluted; Microm), PS100 (polyclonal, 1: 400; DBS), PHOX2B (clone, NC, 1:200, Finetest), BCOR (clone C-10, 1:50, Cliniscience), BRG1 (clone EPNCIR111A, 1:100, Abcam), ETV4 (clone 16, 1:25, Santa-Cruz), NUT (clone C52B1, 1:100, Ozyme), ERG (clone EPR3864, prediluted, Roche).

Picard C., Macagno N., Corradini N., Marec-Bérard P., Cabet S., Guibaud L., Viremouneix L., Raux S., Chotel F., Weinbreck N., Meurgey A., Karanian M., Pissaloux D., Tirode F, & Dijoud F. (2022). Identification of a novel translocation producing an in-frame fusion of TAF15 and ETV4 in a case of extraosseous Ewing sarcoma revealed in the prenatal period. Virchows Archiv : an international journal of pathology, 481(4).

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Immunohistochemical Profiling of Tissue Samples

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The tissue slides were deparaffinized in xylene, hydrated in alcohol, and baked in a microwave (30 min in Tris buffer pH 9). Endogenous peroxidase was blocked. Staining was performed on the Benchmark ultra-automated stainer (Ventana) using diamino-benzidine as chromogen (Dako, Glostrup, Denmark). The following antibodies were used: AE1/E3 (clone PCK26, Ventana), KL1 (clone KL1, HIS-TOLS reagent), EMA (clone E29, Ventana), P63 (4A4, Ventana), S100 protein (clone poly Z311, Dako), SOX10 (EP268, BioSB), Desmin (clone DE-R11, Ventana), Myogenin (clone LO26, Leica), MYOD1 (clone EP212, Cell marque), ALK (clones D5F3, Cell Signaling Technology and clone 1A4, Novocastra), CD99/MIC2 (12E7, Dako), ETV4 (clone 16, Santa Cruz), CD30 (BerH2, Dako), CD4 (4B12, Novocastra), and CD7 (SP94, Ventana).

Le Loarer F., Cleven A.H.G., Bouvier C., Castex M.P., Romagosa C., Moreau A., Salas S., Bonhomme B., Gomez-Brouchet A., Laurent C., Le Guellec S., Audard V., Giraud A., Ramos-Oliver I., Cleton-Jansen A.M., Savci-Heijink D.C., Kroon H.M., Baud J., Pissaloux D., Pierron G., Sherwood A., Coindre J.M., Bovée J.V.M.G., Larousserie F, & Tirode F. (2020). A subset of epithelioid and spindle cell rhabdomyosarcomas is associated with TFCP2 fusions and common ALK upregulation. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 33(3).

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