Oasis wcx

Manufactured by Waters Corporation

Sourced in Germany, United States

The Oasis WCX is a solid-phase extraction (SPE) product from Waters Corporation. It is designed for the extraction and purification of cationic and polar analytes from complex sample matrices. The Oasis WCX utilizes a weak cation-exchange sorbent material to facilitate the extraction process.

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Lab products found in correlation

Oasis hlb, by Waters Corporation (3 mentions) Oasis mcx, by Waters Corporation (2 mentions) Symbia t2 series spect ct scanner, by Siemens (1 mentions) Oasis max, by Waters Corporation (1 mentions) Sep pak vac c18, by Waters Corporation (1 mentions) Strata x c, by Phenomenex (1 mentions) Sep pak vac c8, by Waters Corporation (1 mentions) Vacuum manifold, by Merck Group (1 mentions) Milli q system, by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) Methanol, by Merck Group (1 mentions) Sorbent, by Waters Corporation (1 mentions) Strata x a, by Phenomenex (1 mentions) Chromabond c18, by Macherey-Nagel (1 mentions)

5 protocols using oasis wcx

SPECT Imaging of Striatal D2/3 Receptors

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SPECT data were obtained with a Siemens Symbia T2 series SPECT•CT scanner with low energy high-resolution collimators (full width at half-maximum 7.4mm) and two-slice CT. The ligand, (S)-N-[(1-ethyl-2-pyrrolidinyl)methyl]-2-hydroxy-3-iodo-6-methoxybenzamide ([¹²³I]-IBZM) was chosen due to its selectivity for striatal D_2/3 receptors.^{58 (link),59 (link)} All participants received 185mBq [¹²³I]-IBZM per scanning (GE Healthcare). The SPECT scanning was performed using the constant infusion technique.^{60 (link),61 (link)} A CT scout and 2×30min tomography were performed. The individually adjusted dose of amisulpride was administered to all patients at same time, 3h prior to the SPECT scanning at follow-up.
Plasma free fraction of [¹²³I]-IBZM was determined using ultrafiltration (Centrifree, 30000 MW).^{62 (link)} The plasma metabolite analysis of [¹²³I]-IBZM was performed using Oasis WCX (Waters) solid phase extraction units and stepwise elution with water, 40% acetonitrile and acidified 95% methanol. The native compound was eluted in the water phase and the metabolites in the subsequent elution.
All participants had a structural MRI scan performed for co-registration. The HC were only scanned at baseline to reduce the radiation dose.
Note that the supplementary material contains the details of the SPECT and MRI acquisitions.

Wulff S., Pinborg L.H., Svarer C., Jensen L.T., Nielsen M.Ø., Allerup P., Bak N., Rasmussen H., Frandsen E., Rostrup E, & Glenthøj B.Y. (2015). Striatal D2/3 Binding Potential Values in Drug-Naïve First-Episode Schizophrenia Patients Correlate With Treatment Outcome. Schizophrenia Bulletin, 41(5), 1143-1152.

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Analytical Standards Extraction and Quantification

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Analytical standards TC, OTC, DOX, ENF, LVF, CIP, TYL, TRI, MET, CLR, CLD, SMX, SFD, and VAN were purchased from Sigma-Aldrich (St. Louis, MO, USA). Hypergrade acetonitrile (ACN), methanol (MeOH) and water were purchased from Merck (Darmstadt, Germany). Analytical-grade formic acid (FA), hydrochloric acid (HCl), acetic acid (AcA), sodium hydroxide (NaOH), ethylenediaminetetraacetic acid (EDTA), 25% ammonium hydroxide solution, MeOH, ethanol, ACN and chloroform were purchased from CHEMMPUR (Piekary Śląskie, Poland). Analytical-grade phosphate dibasic dehydrate (>98%) and citrinic acid monohydrate (>98%) were purchased from Sigma-Aldrich.
OASIS HLB (500 mg, 6 mL), OASIS WCX (60 mg, 3 mL), and OASIS MAX (150 mg, 6 mL) cartridges were purchased from Waters (Eschborn, Germany). BAKERBOND^TM Octadecyl (C18) (500 mg, 6 mL) cartridges were purchased from BAKERBOND^® (J.T. Baker, Philipsburg, PA, USA).

Stando K., Korzeniewska E., Felis E., Harnisz M, & Bajkacz S. (2022). Uptake of Pharmaceutical Pollutants and Their Metabolites from Soil Fertilized with Manure to Parsley Tissues. Molecules, 27(14), 4378.

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Solid Phase Extraction Optimization

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The tested extraction phases, Oasis MCX 30 mg/1 mL, Oasis HLB 60 mg/3 mL, Oasis WCX 60 mg/3 mL, Sep-Pak Vac C18 200 mg/3 mL, Sep-Pak Vac C8 200 mg/3 mL, and Sep-Pak Vac C8 500 mg/3 mL, were obtained from Waters (Milford, MA, USA), while the Strata-X-C 60 mg/3 mL was from Phenomenex (Torrance, CA, USA).
Before commencing with the SPE procedure, blank plasma samples were spiked with a working analyte standard solution at the ratio of plasma:standard 9:1 v/v and the samples were diluted with an appropriate solvent. The extractions were carried out using a vacuum manifold (Supelco, Bellefonte, PA, USA) equipped with a vacuum pump. A known volume of the sample eluate (80% of the nominal eluate volume) was evaporated to dryness on a vacuum concentrator (Eppendorf, Hamburg, Germany) and reconstituted in 80 µL of 65% MeOH. Once prepared, the samples were kept on the autosampler at 10 °C for no longer than 10 h.

Turković L., Mutavdžić Pavlović D., Mlinarić Z., Skenderović A., Silovski T, & Sertić M. (2023). Optimisation of Solid-Phase Extraction and LC-MS/MS Analysis of Six Breast Cancer Drugs in Patient Plasma Samples. Pharmaceuticals, 16(10), 1445.

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Quantitative Analysis of Synthetic Cannabinoids

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JWH-122, 5F-AMB, and AMB-PUBINACA were purchased from Shanghai Research Institute of Criminal Science and Technology (Shanghai, China). Acetonitrile and methanol were obtained from Merck (Kenilworth, NJ, USA), and formic acid was purchased from ROE scientific INC (Kenilworth, NJ, USA). All solvents were HPLC grade. Water was purified with a Milli-Q system (Millipore, Bedford, MA, USA) and was used throughout the whole experiment, including sample treatment and instrument-based analysis. Blank rat plasma and urine samples were provided from Zhejiang Academy of Medical Science (Hangzhou, China). The solid phase extraction (SPE) column Oasis HLB (3cc/60 mg), Oasis WCX (3cc/60 mg), and Oasis MCX (3cc/60 mg) were purchased from Waters (Milford, MA, USA).

Ke X., Tian Y., He D., Mu P., Wan X., Zhang L., Jia W., Wang Q., Fan Y, & Zhang Y. (2022). Rapid Simultaneous Determination of Three Synthetic Cannabinoids in Urine and Plasma of Rats Using Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry. Toxics, 10(10), 619.

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Optimization of Plasma Extraction for Bioactive Lipids

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For this study, human plasma was used as biological matrix. Due to its complexity, the efficiency of a suitable extraction procedure was studied by spiking several analytes (LXA4, ATL, LXB4, 12(S)-HETE, 6-keto-PGF1a, ATR, MaR1 and 17(S)-HDoHe) in human plasma. To determine the optimal extraction conditions, several solid phase extraction (SPE) cartridges and procedures were investigated: Chromabond C18 500 mg (Macherey-Nagel GmbH & Co. KG, Weilmünster, Germany), Oasis ® WCX 1 cc, 30 mg sorbent and MCX 1 cc, 30 mg sorbent (Waters, Milford, USA) and Strata-X-A & Strata-XL-A polymer-based sorbent 1 mL, 30 mg (Phenomenex, Aschaffenburg, Germany). The washing solutions examined were sodium acetate (NaAc) and ammonium acetate in different concentrations between 50 and 500 mM as well as a solution consisting of 3% phosphoric acid. For the elution from the cartridges, different concentrations of FA in MeOH were tested.

Toewe A., Balas L., Durand T., Geisslinger G, & Ferreirós N. (2018). Simultaneous determination of PUFA-derived pro-resolving metabolites and pathway markers using chiral chromatography and tandem mass spectrometry. Analytica chimica acta, 1031.

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