Lcms 2010a mass spectrometer

¹H and ¹³C NMR spectroscopic data were recorded on a Bruker DPX 400 spectrometer; chemical shifts (δ) are expressed in parts per million relative to solvent proton as internal standard (CDCl₃-d₁ = 7.26 ppm). LC mass spectrometry was performed on Shimadzu LCMS-2010A mass spectrometer. IR spectra were recorded on JASCO FT/IR-5300 or Nicolet 5700 FT-IR. Elemental analysis was recorded on a Thermo Finnigan Flash EA 1112 analyzer. Spin coating was done on a glass substrate using Laurell TECHNOLOGIES CORPORATION Model WS-400B-6NPP/LITE/8K Spin-coater. UV-Visible absorption and fluorescence spectra were recorded on a spectrophotometer. The solid and liquid state absorbance spectra were collected from a Shimadzu UV-3600 spectrometer and a spectrofluorimeter (Horiba, JobinYvon) respectively. For fluorescence quantum yield measurements, solution of ligands in DCM was matched optically at an absorbing wavelength and then the quantum yield of quinine-sulfate in 0.1 M H₂SO₄ (Φ_f = 0.57, at 22°C) was used as reference. The molecular weights were determined by PSS-Win GPC (PSS) (pump: alliance GPC 2000) GPC equipped with an RI detector using a PL gel MIXED-B column (particle size: 10 mm, dimensions: 0.8 × 30 cm) calibrated against polystyrene standards. For thin-layer chromatography (TLC), silica gel plates Merck 60 F254 were used and compounds were visualized by irradiation with UV light.

Reagents were commercially available and used without further purification except otherwise stated. The monomer 4-pyC₂H₃ was distilled to remove polymerization inhibitor hydroquinone before use. ESI-MS was obtained using a Shimadzu LCMS-2010A mass spectrometer, for which samples were digested by sonication in a mixture of hydrochloric acid and MeOH after removal of the residual monomers by sublimation under vacuum. Elemental analyses (EA) were conducted using an Elementar Vario EL analyzer using guest-free samples (with a little water adsorbed from air). IR spectra were recorded on a Bruker TENSOR 27 FT-IR spectrometer in the 400–4,000 cm⁻¹ region with KBr pellets. ¹H NMR and ¹³C NMR spectra were recorded on a Bruker AVANCE III 400 MHz NMR spectrometer. Chemical shifts were quoted in p.p.m. referenced to the appropriate solvent peak or 0.0 p.p.m. for tetramethylsilicane. PXRD patterns were collected on a Bruker D8-Advance diffractometer with Cu Kα radiation and a LynxEye detector. Thermogravimetry analyses were performed on a TA Q50 system under N₂ at a heating rate of 5 °C min. N₂ sorption isotherms were measured by a Micromeritics ASAP 2020M physisorption analyzer.