Rabbit monoclonal anti aquaporin 1

The ultrasound-guided core needle biopsy sample obtained from # 19’s targeted parotid gland was fixed in 10% formalin and embedded in paraffin. Sections (5 µm) were treated exactly as previously described ^{29 (link)}. The primary antibodies used herein were rabbit monoclonal anti-aquaporin-1, rabbit monoclonal anti-aquaporin-5 and normal rabbit IgG as an antibody control (all from Abcam, Inc., Cambridge, MA, USA). The secondary antibody was Alexa Fluor 488 donkey anti rabbit IgG (H+L) (Invitrogen) and used as described ^{29 (link)}. Additionally, sections of this sample were stained conventionally with hematoxylin and eosin (H&E).

Submandibular glands from mice and rats were fixed in 10% formalin and embedded in paraffin. Sections (5 um) were deparaffinized and rehydrated in a graded series of ethanol, then washed in phosphate buffered saline. Antigen retrieval was carried out with 1 mmol l ⁻¹ EDTA (pH 8) and 0.05% Tween 20 in a microwave oven for 10 min. Sections were then blocked with 20% goat serum in 5% bovine serum albumin for 1 h, incubated with primary antibodies, either rabbit monoclonal anti-aquaporin-1 or normal rabbit IgG as an antibody control (Abcam, Inc., Cambridge, MA, USA), in 5% bovine serum albumin in phosphate buffered saline for 1 h at room temperature and washed with phosphate buffered saline. Next, the slides were incubated with secondary antibody—Alexa Fluor 488 donkey anti rabbit IgG (H+L) (Invitrogen) for 1 h, washed with phosphate buffered saline and mounted with Prolong Gold antifade reagent with DAPI (4′, 6-diamidino-2-phenylindole; Invitrogen).