Serum adiponectin

Manufactured by R&D Systems

Sourced in United States

Serum adiponectin is a protein biomarker that can be measured using laboratory equipment. It reflects the concentration of adiponectin, an adipokine secreted by adipose tissue, in the serum or blood sample.

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Lab products found in correlation

Elisa kit, by R&D Systems (2 mentions) P selectin, by R&D Systems (1 mentions) Icam 1, by R&D Systems (1 mentions) Tba 200fr, by Toshiba (1 mentions) Elecsys autoanalyzer, by Roche (1 mentions)

4 protocols using serum adiponectin

Fasting Blood Biomarker Analysis

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After a minimum of ten hours overnight fasting, blood samples were obtained from every participant. The blood samples were centrifuged at 3,400 rounds per minute for ten minutes in 4℃. The serum was stored at -70℃.
The serum was melted at room temperature and prepared for enzyme-linked immunosorbent assay tests of serum adiponectin (R&D systems, Minneapolis, MN, USA), ICAM-1 (R&D systems), CD40 (R&D systems), and P-Selectin (R&D systems). Total cholesterol, HDL, LDL, triglyceride, aspartate aminotransferase (AST), alanine aminotransferase (ALT), CRP, fasting glucose and serum insulin (Toshiba TBA 200FR, Toshiba Medical Systems, Tokyo, Japan) were also measured. Homeostasis model assessment (HOMA-IR) was calculated by the following equation (fasting serum insulin (mU/L)×fasting glucose (mmol/L)/22.5).

Byun S.H., Kwon E.B, & Kim S.Y. (2014). The relationship between serum adiponectin and inflammatory cytokines in obese Korean juveniles. Korean Journal of Pediatrics, 57(12), 533-537.

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Obesity Biomarkers in Rat Model

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The body mass and body length (from the tip of the nose to anus) of rats were measured at the end of 6 months of feeding period. BMI (kg/m²) = body mass/body length².
For the determination of serum adiponectin, interleukin 6 (IL-6), tumor necrosis factor-α (TNF-α) and 4-hydroxy 2-nonenal (HNE), 5 ml of blood sample was drawn from abdominal aorta. Blood samples for serum collection were immediately centrifuged at 3000 r/min for 15 min and aliquots were stored at −80°C. The concentrations of serum adiponectin (R and D Systems; Minneapolis, USA), IL-6 (Blue Gene; Shanghai, China), TNF-α (Blue Gene; Shanghai, China), and 4-HNE (Blue Gene; Shanghai, China) were determined by enzyme-linked immunosorbent assay (ELISA) as recommended by the manufacturers.

Wang R.Y., Liu H., Ma L.J, & Xu J.Y. (2016). Comparison of Serum Adiponectin in Smoke-induced Pulmonary Emphysema Rats Fed Different Diets. Chinese Medical Journal, 129(2), 187-193.

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Metabolic Biomarkers in Humans

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Serum triglyceride (TG), HDL-cholesterol (HDL-c), and plasma glucose (FPG) were manually measured by their respective kits (Human Company, Germany). Insulin was measured by Electrochemiluminescence immunoassay (ECL) on Elecsys autoanalyzer (Roche Diagnostics, Germany). Insulin resistance and Insulin sensitivity (IS) were calculated using the Homeostasis Model Assessment (HOMA 2) Calculator v2.2 which is available from Oxford Centre for Diabetes, Endocrinology and Metabolism. ELISA kits were used to measure serum adiponectin (R&D Systems, USA), serum leptin and hs-CRP (DRG Diagnostics, Germany).

Al-Hamodi Z., AL-Habori M., Al-Meeri A, & Saif-Ali R. (2014). Association of adipokines, leptin/adiponectin ratio and C-reactive protein with obesity and type 2 diabetes mellitus. Diabetology & Metabolic Syndrome, 6, 99.

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Fasting Lipid and Adipokine Profiles

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After a 12-h fasting, 10 ml/l of vein blood sample was obtained from each patient and after centrifuging by 1000 g for 10 min, and the samples were stored at − 70 °C. Lipid profiles, including triglycerides (TGs), cholesterol (Chol), low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C), and plasma glucose were measured using routine laboratory techniques (Pars Azmoon kit, Catalog No 1500017, Tehran, Iran for TG and Chol and Zist Shimi kit, Serial No D00749, Tehran, Iran for HDL-C respectively). LDL-C was estimated based on direct measurement with autoanalyzer. ELISA kits were used to assay the ratios of serum adiponectin (R&D Systems, USA) and serum leptin (D&G Diagnostics, Germany), and the assays were conducted according to the manufacturer’s protocol.

Rahmani A., Toloueitabar Y., Mohsenzadeh Y., Hemmati R., Sayehmiri K, & Asadollahi K. (2020). Association between plasma leptin/adiponectin ratios with the extent and severity of coronary artery disease. BMC Cardiovascular Disorders, 20, 474.

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