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Mouse anti sarcomeric alpha actinin

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The Mouse anti-sarcomeric alpha-actinin is a laboratory reagent used for the detection and localization of sarcomeric alpha-actinin, a key structural protein found in the Z-discs of muscle cells. This monoclonal antibody is a useful tool for researchers studying muscle cell structure and function.

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Lab products found in correlation

Lsm 510 meta, by Zeiss (8 mentions) Mouse anti cardiac troponin t, by Thermo Fisher Scientific (4 mentions) Rabbit anti cardiac troponin t, by Abcam (4 mentions) Zen software, by Zeiss (4 mentions) Mouse anti flag m2, by Merck Group (2 mentions) Rabbit anti tbx20, by Abcam (2 mentions) Eclipse 80i fluorescent microscope, by Zeiss (2 mentions) Nis elements basic research 3, by Nikon (2 mentions) Citrate buffer, by Merck Group (2 mentions) Mouse anti tnnt2, by Thermo Fisher Scientific (2 mentions) Rabbit anti ki67, by Thermo Fisher Scientific (2 mentions) Rabbit anti tnni3, by Santa Cruz Biotechnology (2 mentions) Lsm 510 meta inverted confocal microscope, by Zeiss (2 mentions) Rabbit anti phospho smad2, by Merck Group (2 mentions) Rabbit anti prdm16, by Abcam (2 mentions) Rabbit anti phospho histone h3, by Cell Signaling Technology (2 mentions) Rabbit anti tgf β1, by Abcam (2 mentions) Eclipse 80i fluorescent microscope, by Nikon (2 mentions) Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions) Eclipse 80i, by Zeiss (2 mentions)

8 protocols using mouse anti sarcomeric alpha actinin

1

Immunofluorescence Analysis of Cardiac Development

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Mouse embryos and neonates were collected, fixed in 4% PFA, embedded in OCT, and frozen in dry iced hexane. Frozen sections were blocked with 5% goat serum, stained with mouse anti-TNNT2 (Thermo Scientific), mouse anti-sarcomeric alpha-actinin (Sigma Aldrich), rabbit anti-TNNI3 (Santa Cruz), rabbit anti-TGFβ1 (Abcam), rabbit anti-Ki67 (Thermo Scientific), rabbit anti-phospho-Histone H3 (Cell Signaling Technology), and Hoechst 33342, and visualized using Alexa Fluor-conjugated secondary antibodies (Life Technologies). Image acquisition was performed on a Zeiss LSM510 Meta inverted confocal microscope (Carl Zeiss) or an Eclipse 80i fluorescent microscope (Nikon). Transgenes were detected by PCR from yolk sac DNA of embryos or tail DNA of neonatal pups. For human heart tissues, formalin-fixed paraffin embedded tissue-sections were rehydrated and autoclaved with Citrate Buffer, pH 6.0 (Sigma-Aldrich) and stained with rabbit anti-phospho-Smad2 (EMD Millipore), rabbit anti-PRDM16 (Abcam), mouse anti-sarcomeric alpha-actinin (Sigma Aldrich) and Hoechst 33342, and visualized using Alexa Fluor-conjugated secondary antibodies (Life Technologies). Image acquisition was performed on an Eclipse 80i fluorescent microscope (Nikon). A detailed list of antibodies used is shown in Supplementary Table 10.
Kodo K., Ong S.G., Jahanbani F., Termglinchan V., Hirono K., InanlooRahatloo K., Ebert A.D., Shukla P., Abilez O.J., Churko J.M., Karakikes I., Jung G., Ichida F., Wu S.M., Snyder M.P., Bernstein D, & Wu J.C. (2016). iPSC-derived cardiomyocytes reveal abnormal TGFβ signaling in left ventricular non-compaction cardiomyopathy. Nature cell biology, 18(10), 1031-1042.
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2

Immunofluorescence Analysis of Cardiac Development

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Mouse embryos and neonates were collected, fixed in 4% PFA, embedded in OCT, and frozen in dry iced hexane. Frozen sections were blocked with 5% goat serum, stained with mouse anti-TNNT2 (Thermo Scientific), mouse anti-sarcomeric alpha-actinin (Sigma Aldrich), rabbit anti-TNNI3 (Santa Cruz), rabbit anti-TGFβ1 (Abcam), rabbit anti-Ki67 (Thermo Scientific), rabbit anti-phospho-Histone H3 (Cell Signaling Technology), and Hoechst 33342, and visualized using Alexa Fluor-conjugated secondary antibodies (Life Technologies). Image acquisition was performed on a Zeiss LSM510 Meta inverted confocal microscope (Carl Zeiss) or an Eclipse 80i fluorescent microscope (Nikon). Transgenes were detected by PCR from yolk sac DNA of embryos or tail DNA of neonatal pups. For human heart tissues, formalin-fixed paraffin embedded tissue-sections were rehydrated and autoclaved with Citrate Buffer, pH 6.0 (Sigma-Aldrich) and stained with rabbit anti-phospho-Smad2 (EMD Millipore), rabbit anti-PRDM16 (Abcam), mouse anti-sarcomeric alpha-actinin (Sigma Aldrich) and Hoechst 33342, and visualized using Alexa Fluor-conjugated secondary antibodies (Life Technologies). Image acquisition was performed on an Eclipse 80i fluorescent microscope (Nikon). A detailed list of antibodies used is shown in Supplementary Table 10.
Kodo K., Ong S.G., Jahanbani F., Termglinchan V., Hirono K., InanlooRahatloo K., Ebert A.D., Shukla P., Abilez O.J., Churko J.M., Karakikes I., Jung G., Ichida F., Wu S.M., Snyder M.P., Bernstein D, & Wu J.C. (2016). iPSC-derived cardiomyocytes reveal abnormal TGFβ signaling in left ventricular non-compaction cardiomyopathy. Nature cell biology, 18(10), 1031-1042.
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3

Fluorescent Imaging of Sarcomeric Proteins

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NRCs were transiently transfected with GFP-tagged C3C6 constructs using Escort III (Sigma) as described previously (31 (link)) and then fixed and permeabilized as aforementioned. Immunostaining was carried out using primary antibodies against mouse antisarcomeric alpha-actinin (Sigma) and rabbit anti MyBP-C (a generous gift from Prof Mathias Gautel, Kings College London) and secondary antibody/counterstain solution (Cy3-goat anti-mouse, catalog no.: 115-165-146; Jackson Immunochemicals) and Atto 647N-goat anti-rabbit (Sigma). Cells were mounted using 70% glycerol/PBS according to the antibody manufacturer instructions. Samples were imaged using a STEDYCON (Abberior) attached to a Leica TCS SP5 confocal microscope equipped with 594 nm and 640 nm excitation lasers and a 775 nm depletion laser using a 100×/numerical aperture 1.4 oil immersion lens. Images were recorded at a pixel size of 20 nm.
Pearce A., Ponnam S., Holt M.R., Randall T., Beckingham R., Kho A.L., Kampourakis T, & Ehler E. (2023). Missense mutations in the central domains of cardiac myosin binding protein-C and their potential contribution to hypertrophic cardiomyopathy. The Journal of Biological Chemistry, 300(1), 105511.
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4

Immunofluorescence Staining Protocol for Cardiac Cells

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Cells grown on cover slips were fixed using 4% PFA, permeabilized with 0.5% Triton X-100, incubated with primary antibodies and Hoechst 33342, and detected using Alexa Fluor conjugated secondary antibodies. Primary antibodies used include mouse anti-FLAG M2 (Sigma Aldrich), rabbit anti-cardiac troponin T (Abcam), mouse anti-cardiac troponin T (Thermo Scientific), mouse anti-sarcomeric alpha-actinin (Sigma Aldrich), or rabbit anti-TBX20 (Abcam), as published previously45 (link). Image acquisition was performed on an Eclipse 80i fluorescent microscope and a confocal microscope (Carl Zeiss, LSM 510 Meta) and ZEN software (Carl Zeiss). Measurement of cell surface area and length was performed using NIS-Elements Basic Research 3.0 software (Nikon). A detailed list for antibodies used is shown in Supplementary Table 10.
Kodo K., Ong S.G., Jahanbani F., Termglinchan V., Hirono K., InanlooRahatloo K., Ebert A.D., Shukla P., Abilez O.J., Churko J.M., Karakikes I., Jung G., Ichida F., Wu S.M., Snyder M.P., Bernstein D, & Wu J.C. (2016). iPSC-derived cardiomyocytes reveal abnormal TGFβ signaling in left ventricular non-compaction cardiomyopathy. Nature cell biology, 18(10), 1031-1042.
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5

Immunofluorescence Analysis of Cardiac Cells

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Cells grown on coverslips were fixed using 4% PFA, permeabilized with 0.5% Triton X-100, incubated with primary antibodies and Hoechst 33342, and detected using Alexa Fluor-conjugated secondary antibodies. Primary antibodies include rabbit anti-cardiac troponin T (Abcam), mouse anti-cardiac troponin T (Thermo Scientific), mouse anti-sarcomeric alpha-actinin (Sigma-Aldrich), goat anti-LMNA (Santa Cruz), and rabbit anti-LMNA (Santa Cruz). Image acquisition was performed on an Eclipse 80i fluorescence microscope, a confocal microscope (Carl Zeiss, LSM 510 Meta), and ZEN software (Carl Zeiss).
Lee J., Termglinchan V., Diecke S., Itzhaki I., Lam C.K., Garg P., Lau E., Greenhaw M., Seeger T., Wu H., Zhang J.Z., Chen X., Gil I.P., Ameen M., Sallam K., Rhee J.W., Churko J., Chaudhary R., Chour T., Wang P.J., Snyder M.P., Chang H.Y., Karakikes I, & Wu J.C. (2019). Activation of PDGF Pathway Links LMNA Mutation to Dilated Cardiomyopathy. Nature, 572(7769), 335-340.
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6

Comprehensive Antibody Catalog for Protein Analysis

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Rabbit anti-CSN1, −CSN2 and –CSN8 were purchased from ENZO life Sciences (Farmingdale, NY). Rabbit anti-CSN3 and mouse anti-CSN5 (Jab1, ab495) were obtained from Bethyl Laboratories (Montgomery, TX) and Abcam (Cambridge, MA) respectively. Mouse anti-myogenin was purchased from BD Biosciences (San Jose, CA). Bizbenzimide and mouse anti-sarcomeric alpha actinin were purchased from Sigma (St. Louis, MO). Mouse anti-myosin heavy chain-clone MF-20 was obtained from Hybridoma Bank (Iowa City, Iowa). The mouse anti-tubulin, mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and NF-κB antibodies were from Santa Cruz Biotechnologies (Santa Cruz, CA), and p21, p26 and CD6 from Cell Signaling Technology (Danvers, MA). Secondary antibodies used in immunoblotting were from LI-COR Biosciences (USA), and those used in immunostaining were from Invitrogen Molecular Probes (Eugene, OR).
Ba M.A., Surina J., Singer C.A, & Valencik M.L. (2017). Knockdown of subunit 3 of the COP9 signalosome inhibits C2C12 myoblast differentiation via NF-KappaB signaling pathway. BMC Pharmacology & Toxicology, 18, 47.
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7

Immunofluorescence Analysis of Cardiac Cells

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Cells grown on coverslips were fixed using 4% PFA, permeabilized with 0.5% Triton X-100, incubated with primary antibodies and Hoechst 33342, and detected using Alexa Fluor-conjugated secondary antibodies. Primary antibodies include rabbit anti-cardiac troponin T (Abcam), mouse anti-cardiac troponin T (Thermo Scientific), mouse anti-sarcomeric alpha-actinin (Sigma-Aldrich), goat anti-LMNA (Santa Cruz), and rabbit anti-LMNA (Santa Cruz). Image acquisition was performed on an Eclipse 80i fluorescence microscope, a confocal microscope (Carl Zeiss, LSM 510 Meta), and ZEN software (Carl Zeiss).
Lee J., Termglinchan V., Diecke S., Itzhaki I., Lam C.K., Garg P., Lau E., Greenhaw M., Seeger T., Wu H., Zhang J.Z., Chen X., Gil I.P., Ameen M., Sallam K., Rhee J.W., Churko J., Chaudhary R., Chour T., Wang P.J., Snyder M.P., Chang H.Y., Karakikes I, & Wu J.C. (2019). Activation of PDGF Pathway Links LMNA Mutation to Dilated Cardiomyopathy. Nature, 572(7769), 335-340.
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8

Immunofluorescence Staining Protocol for Cardiac Cells

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Cells grown on cover slips were fixed using 4% PFA, permeabilized with 0.5% Triton X-100, incubated with primary antibodies and Hoechst 33342, and detected using Alexa Fluor conjugated secondary antibodies. Primary antibodies used include mouse anti-FLAG M2 (Sigma Aldrich), rabbit anti-cardiac troponin T (Abcam), mouse anti-cardiac troponin T (Thermo Scientific), mouse anti-sarcomeric alpha-actinin (Sigma Aldrich), or rabbit anti-TBX20 (Abcam), as published previously45 (link). Image acquisition was performed on an Eclipse 80i fluorescent microscope and a confocal microscope (Carl Zeiss, LSM 510 Meta) and ZEN software (Carl Zeiss). Measurement of cell surface area and length was performed using NIS-Elements Basic Research 3.0 software (Nikon). A detailed list for antibodies used is shown in Supplementary Table 10.
Kodo K., Ong S.G., Jahanbani F., Termglinchan V., Hirono K., InanlooRahatloo K., Ebert A.D., Shukla P., Abilez O.J., Churko J.M., Karakikes I., Jung G., Ichida F., Wu S.M., Snyder M.P., Bernstein D, & Wu J.C. (2016). iPSC-derived cardiomyocytes reveal abnormal TGFβ signaling in left ventricular non-compaction cardiomyopathy. Nature cell biology, 18(10), 1031-1042.
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