Du 730 nucleic acid

Optical rotations were obtained using an Anton Paar MCP 200 polarimeter. Ultraviolet spectra were obtained with a Beckman Coulter DU 730 nucleic acid/protein analyzer. CD spectra were measured on a Biologic MOS-450 spectra polarimeter (Biologic Science, Claix, France). IR spectra were performed on a Bruker Tensor 27 FT-IR spectrophotometer (film). NMR spectra were recorded on a Bruker AV-600 spectrometer using tetramethylsilane as an internal standard, and the chemical shifts were recorded in δ values. HRESIMS were measured using a Bruker micro TOF-Q mass instrument (Bruker Daltonics, Billerica, MA, USA). ESIMS were performed on an Agilent 1290-6420 Triple Quadrupole LC-MS spectrometer. Silica gel (300–400 mesh, and 1200–1500 mesh, Qingdao Marine Chemical Ltd., Qingdao, China), Sephadex LH-20 (GE Healthcare Biosciences AB, Uppsala, Sweden), YMC*GEL ODS-A (S-50 μm, 12 nm) (YMC Co., Ltd., Kyoto, Japan), and MCI gel (CHP-20P, Mitsubishi Chemical Corp., Tokyo, Japan) were utilized for column chromatography. Semipreparative HPLC was performed using a ODS column (250 mm × 10 mm, 5 μm, YMC-ODS-A). Unless otherwise specified, all chemicals and solvents were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shenyang, China). Biological assays were analyzed using a microplate reader (BioTek Synergy H1, BioTek Instruments, Inc., Vermont, USA).

Optical rotation were determined using an Anton Paar MCP200 automatic polarimeter (Graz, Austria). Ultraviolet spectra were obtained with a Beckman Coulter DU 730 nucleic acid/protein analyzer. IR spectra were recorded with a Bruker Tensor 27 FT-IR spectrometer. ESI-MS were recorded on an Agilent 1290–6420 Triple Quadrupole LC-MS spectrometer (Santa Clara, CA, USA). HRESI-MS experiments were performed using a Bruker Micro TOF-Q mass spectrometer (Bruker Daltonics, Billerica, MA). NMR spectra were recorded on a Bruker Advance III-600 MHz spectrometer (Bruker, Rheinstetten, Germany). Flow cytometric analysis were conducted on an LSR-Fortessa flow cytometer (BD, Franklin Lakes, NJ, USA). MTT and protein quantification were analyzed by a microplate reader (BioTek Synergy H1, BioTek Instruments, Inc., Vermont, USA). Silica gel (100–200 mesh, 200–300 mesh, Qingdao Marine Chemical, Ltd., Qingdao, China), MCI gel (CHP-20P, Mitsubishi Chemical Corp., Tokyo, Japan), and YMC*GEL ODS-A (S-50 μm, 12 nm) (YMC Co., Ltd., Kyoto, Japan) were used for column chromatography. The primary antibodies for cyclin A2, CDK2, p21, Bax, Bcl-2, caspase-3, GAPDH, and horse-radish peroxidase (HRP)-conjugated secondary antibodies were purchased from Cell Signaling Technology (Dancers, MA, USA).