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Acquity uplc hss t3 c18

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The ACQUITY UPLC HSS T3 C18 is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of a wide range of compounds. The column features a silica-based stationary phase with a particle size of 1.8 μm, which allows for high-resolution separations and fast analysis times. The C18 bonded phase provides a versatile and well-characterized stationary phase for the separation of a variety of analytes, including polar and non-polar compounds.

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Lab products found in correlation

6500 q trap, by Thermo Fisher Scientific (25 mentions) Shim pack uflc cbm30a system, by Shimadzu (23 mentions) 4500 q trap, by Thermo Fisher Scientific (15 mentions) Cbm30a system, by Thermo Fisher Scientific (12 mentions) Analyst 1, by AB Sciex (12 mentions) 6500 triple quadrupole, by Thermo Fisher Scientific (8 mentions) Scaa 104, by ANPEL (8 mentions) Qtrap system, by AB Sciex (6 mentions) Cnwbond carbon gcb spe cartridge, by ANPEL (6 mentions) Cbm30a, by Shimadzu (3 mentions) Exionlc ad, by AB Sciex (3 mentions) Multiquant 3, by AB Sciex (3 mentions) Qtrap lc ms ms system, by AB Sciex (2 mentions) Qtrap 6500 system, by Waters Corporation (2 mentions) Cbm30a system, by Shimadzu (2 mentions) Exionlc ad, by Thermo Fisher Scientific (2 mentions) Shim pack uflc cbm30a, by Shimadzu (2 mentions) Qtrap 6500 system, by Shimadzu (2 mentions) Qtrap 6500, by AB Sciex (2 mentions) Api6500 q trap, by AB Sciex (1 mentions)

Spelling variants of this product

Acquity UPLC (716 citations) ACQUITY UPLC HSS T3 (206 citations) ACQUITY UPLC HSS T3 C18 column (67 citations) HSS T3 (52 citations) Acquity HSS T3 (29 citations) UPLC HSS T3 (12 citations) Acquity UPLC HSS C18 (8 citations) HSS T3 C18 (8 citations) ACQUITY UPLC HSS T3 C18 (1.8 μm (2 citations) Waters Acquity UPLC HSS T3 C18 column (2 citations)

132 protocols using acquity uplc hss t3 c18

1

LC-ESI-MS/MS Analysis of Samples

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The sample extracts were analyzed using an LC-ESI-MS/MS system (HPLC, Shim-pack UFLC SHIMADZU CBM30A system, www.shimadzu.com.cn/, accessed on 8 January 2022; MS, Applied Biosystems 6500 Q TRAP, www.appliedbiosystems.com.cn/, accessed on 8 January 2022). The analytical conditions were as follows, HPLC: column, Waters ACQUITY UPLC HSS T3 C18 (1.8 µm, 2.1 mm × 100 mm); solvent system, water (0.04% acetic acid): acetonitrile (0.04% acetic acid); gradient program, 95:5 v/v at 0 min, 5:95 v/v at 11.0 min, 5:95 v/v at 12.0 min, 95:5 v/v at 12.1 min, 95:5 v/v at 15.0 min; flow rate, 0.40 mL/min; temperature, 40 °C; injection volume: 2 μL. The effluent was alternatively connected to an ESI-triple quadrupole-linear ion trap (Q TRAP)-MS.
Yue E., Huang Y., Qian L., Lu Q., Wang X., Qian H., Yan J, & Ruan S. (2022). Comparative Analysis of Proanthocyanidin Metabolism and Genes Regulatory Network in Fresh Leaves of Two Different Ecotypes of Tetrastigma hemsleyanum. Plants, 11(2), 211.
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2

LC-ESI-MS/MS Metabolite Profiling

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The sample extracts were analyzed using an LC-ESI-MS/MS system (UPLC, Shim-pack UFLC SHIMADZU CBM A system, https://www.shimadzu.com/; MS, QTRAP® System, https://sciex.com/). The analytical conditions were as follows, UPLC: column, Waters ACQUITY UPLC HSS T3 C18 (1.8 μm, 2.1 mm * 100 mm); column temperature, 40°C; flow rate, 0.4 ml/min; injection volume, 2 μl; solvent system, water (0.04% acetic acid): acetonitrile (0.04% acetic acid); gradient program, 95:5 V/V at 0 min, 5:95 V/V at 11.0 min, 5:95 V/V at 12.0 min, 95:5 V/V at 12.1 min, 95:5 V/V at 14.0 min. LIT and triple quadrupole (QQQ) scans were acquired on a triple quadrupole-linear ion trap mass spectrometer (QTRAP), QTRAP® LC-MS/MS System, equipped with an ESI Turbo Ion-Spray interface, operating in positive and negative ion mode and controlled by Analyst 1.6.3 software (Sciex). The ESI source operation parameters were as follows: source temperature 500°C; ion spray voltage (IS) 5,500 V (positive), −4,500 V (negative); ion source gas I (GSI), gas II (GSII), curtain gas (CUR) was set at 55, 60, and 25.0 psi, respectively; the collision gas (CAD) was high. Instrument tuning and mass calibration were performed with 10 and 100 μmol/L polypropylene glycol solutions in QQQ and LIT modes, respectively. A specific set of MRM transitions were monitored for each period according to the metabolites eluted within this period.
Lin J., Ge L., Mei X., Niu Y., Chen C., Hou S, & Liu X. (2022). Integrated ONT Full-Length Transcriptome and Metabolism Reveal the Mechanism Affecting Ovulation in Muscovy Duck (Cairina moschata). Frontiers in Veterinary Science, 9, 890979.
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3

UPLC-MS/MS Analysis of Compound Extracts

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A LC-ESI-MS/MS system (UPLC, ExionLC AD, https://sciex.com.cn/; MS, QTRAP® System, https://sciex.com/) was used to analyze the sample extracts. Here are the conditions for the analysis, UPLC: column, Waters ACQUITY UPLC HSS T3 C18 (1.8 μm, 2.1 mm*100 mm); column temperature, 40 °C; flow rate, 0.4 mL/min; injection volume, 2 µL; solvent system, water (0.1% formic acid): acetonitrile (0.1% formic acid); gradient program, 95:5 V/V at 0 min, 10:90 V/V at 10.0 min, 10:90 V/V at 11.0 min, 95:5 V/V at 11.1 min, 95:5 V/V at 14.0 min.
Zhou X., Huang G., Wang L., Zhao Y., Li J., Chen D., Wei L., Chen Z, & Yang B. (2023). L-carnitine promotes liver regeneration after hepatectomy by enhancing lipid metabolism. Journal of Translational Medicine, 21, 487.
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4

LC-ESI-MS/MS Analytical Conditions

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The sample extracts were analyzed using the LC-ESI-MS/MS system (HPLC, Shim-pack UFLC SHIMADZU CBM30A system, www.shimadzu.com.cn/; MS, Applied Biosystems 6500 Q TRAP). The analytical conditions were as follows: Column, Waters ACQUITY UPLC HSS T3 C18 (1.8 µm, 2.1 × 100 mm); solvent system, water (0.04% acetic acid): Acetonitrile (0.04% acetic acid); gradient program, 95:5 (v/v) at 0 min, 5:95 (v/v) at 11.0 min, 5:95 (v/v) at 12.0 min, 95:5 (v/v) at 12.1 min, 95:5 (v/v) at 15.0 min; flow rate, 0.40 mL·min−1; temperature, 40 °C; injection volume: 2 μL. The effluent was alternatively connected to the ESI-triple quadrupole-linear ion trap (Q TRAP)-MS.
Luo Y., Ge C., Yang M., Long Y., Li M., Zhang Y., Chen Q., Sun B., Wang Y., Wang X, & Tang H. (2020). Cytosolic/Plastid Glyceraldehyde-3-Phosphate Dehydrogenase Is a Negative Regulator of Strawberry Fruit Ripening. Genes, 11(5), 580.
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5

LC-ESI-MS/MS Analysis of Samples

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The sample extracts were analyzed using an LC-ESI-MS/MS system (HPLC, Shim-pack UFLC SHIMADZU CBM30A system, www.shimadzu.com.cn/ accessed on 11 February 2019; MS, Applied Biosystems 6500 Q TRAP, www.appliedbiosystems.com.cn/ accessed on 17 February 2019). The analytical conditions were as follows, HPLC: column, Waters ACQUITY UPLC HSS T3 C18 (1.8 µm, 2.1 mm × 100 mm); solvent system, water (0.04% acetic acid): acetonitrile (0.04% Acetic acid); gradient program,95:5 v/v at 0 min, 5:95 v/v at 11.0 min, 5:95 v/v at 12.0 min, 95:5 v/v at 12.1 min, 95:5 v/v at 15.0 min; flow rate, 0.40 mL/min; temperature, 40 °C; injection volume: 2 μL. The effluent was alternatively connected to an ESI-triple quadrupole-linear ion trap (Q TRAP)-MS.
Cheng H., Zha S., Luo Y., Li L., Wang S., Wu S., Cheng S, & Li L. (2022). JAZ1-3 and MYC2-1 Synergistically Regulate the Transformation from Completely Mixed Flower Buds to Female Flower Buds in Castanea mollisima. International Journal of Molecular Sciences, 23(12), 6452.
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6

Quantitative LC-MS/MS Analysis Protocol

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The sample extracts were analyzed using an LC-ESI-MS/MS system (UPLC, ExionLC AD, https://sciex.com.cn/; MS, QTRAP® System, https://sciex.com/). The analytical conditions were as follows: UPLC: column, Waters ACQUITY UPLC HSS T3 C18 (1.8 μm, 2.1 mm × 100 mm); column temperature, 40 °C; flow rate, 0.4 mL/min; injection volume, 2 μL; solvent system, water (0.1% formic acid): acetonitrile (0.1% formic acid); gradient program, 95:5 V/V at 0 min, 10:90 V/V at 11.0 min, 10:90 V/V at 12.0 min, 95:5 V/V at 12.1 min, and 95:5 V/V at 14.0 min.
Wang S., Chen J., Li H., Qi X., Liu X, & Guo X. (2021). Metabolomic Detection Between Pancreatic Cancer and Liver Metastasis Nude Mouse Models Constructed by Using the PANC1-KAI1/CD82 Cell Line. Technology in Cancer Research & Treatment, 20, 15330338211045204.
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7

LC-ESI-MS/MS Analysis of Compounds

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The sample extracts were analyzed using an LC-ESI-MS/MS system (HPLC: Shim-pack UFLC SHIMADZU CBM30A system, www.shimadzu.com.cn/; MS: Applied Biosystems 6500 Q TRAP, www.appliedbiosystems.com.cn/). The analytical conditions were as follows: HPLC column, Waters ACQUITY UPLC HSS T3 C18 (1.8 μm, 2.1 × 100 mm); solvent system, water (0.04% acetic acid) and acetonitrile (0.04% acetic acid); gradient program, 95:5 v/v at 0 min, 5:95 v/v at 11.0 min, 5:95 v/v at 12.0 min, 95:5 v/v at 12.1 min, 95:5 v/v at 15.0 min; flow rate, 0.40 mL/min; temperature, 40 °C; injection volume, 2 μL. The effluent was alternatively connected to an ESI-triple quadrupole-linear ion trap (Q TRAP)-MS.
Guo H., Guo H., Zhang L., Tang Z., Yu X., Wu J, & Zeng F. (2019). Metabolome and Transcriptome Association Analysis Reveals Dynamic Regulation of Purine Metabolism and Flavonoid Synthesis in Transdifferentiation during Somatic Embryogenesis in Cotton. International Journal of Molecular Sciences, 20(9), 2070.
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8

Metabolite Profiling via UPLC-MS/MS

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Extraction and sample preparation were performed according to a protocol described previously [33 (link)]. The data acquisition instrument system mainly includes Ultra Performance Liquid Chromatography (Shim-pack UFLC, Shimadzu CBM30A) and Tandem Mass Spectrometry (MS/MS) (Applied Biosystems 6500 QTRAP). The UPLC analytical conditions were as follows: column, Waters ACQUITY UPLC HSS T3 C18 (1.8 μm, 2.1 mm × 100 mm); solvent system, water (0.04% acetic acid): acetonitrile (0.04% acetic acid); gradient program, 100:0 V/V at 0 min, 5:95 V/V at 11.0 min, 5:95 V/V at 12.0 min, 95:5 V/V at 12.1 min, 95:5 V/V at 15.0 min; flow rate, 0.40 mL/min; temperature, 40 °C; injection volume: 2 μL. The MS conditions mainly include ESI, 500 °C; CUR, 25 psi. Metabolite quantification was performed using a scheduled multiple reaction monitoring (MRM) method [34 (link)].
Xiong Y., He J., Li M., Du K., Lang H., Gao P, & Xie Y. (2023). Integrative Analysis of Metabolome and Transcriptome Reveals the Mechanism of Color Formation in Yellow-Fleshed Kiwifruit. International Journal of Molecular Sciences, 24(2), 1573.
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9

Quantitative Analysis of Compounds via LC-ESI-MS/MS

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An LC-ESI-MS/MS system was used to analyze the samples. The whole analytical system conditions are described as follows. The HPLC column was purchased from Waters ACQUITY UPLC HSS T3 C18 (1.8 μm, 2.1 mm × 100 mm). The composition and proportion of the solvent system was water (0.04% acetic acid):acetonitrile (0.04% acetic acid). The gradient programming was set as follows: 0 min 100:0v/v, 11.0 min 5:95v/v, 12.0 min 5:95v/v, 12.1 min 95:5v/v, 15.0 min 95:5v/v. The temperature was 40 °C and the flow rate was set as 0.40 mL/min. The injection volume was 5 microliters. The effluent of the sample was alternatively connected to the ESI-triple quadrupole-linear ion trap (Q TRAP)-MS.
Liang S., Zhang J., Liu Y., Wen Z., Liu X., Dang F., Xie T., Wang J., Wang Z, & Wu H. (2022). Study on Flavonoids and Bioactivity Features of Pericarp of Citrus reticulata “Chachi” at Different Harvest Periods. Plants, 11(23), 3390.
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10

UPLC-ESI-MS/MS Analysis of Metabolites

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The sample extracts were analyzed using an UPLC−ESI−MS/MS system (UPLC, Shim−pack UFLC SHIMADZU CBM30A system; MS, Applied Biosystems 4500 Q TRAP) [64 (link)]. The analytical conditions were as follows: UPLC column, Waters ACQUITY UPLC HSS T3 C18 (1.8 µm, 2.1 mm × 100 mm); the mobile phase consisted of 0.04% acetic acid aqueous solution solvent A and 0.04% acetic acid acetonitrile solvent B; the gradient procedure was used for sample measurement, and the initial conditions were 95% A and 5% B. Within 10 min, the combination of linear gradient to 5% A, 95% B, 5% A and 95% B were maintained for 1 min.
Feng K., Kan X.Y., Liu Q., Yan Y.J., Sun N., Yang Z.Y., Zhao S.P., Wu P, & Li L.J. (2023). Metabolomics Analysis Reveals Metabolites and Metabolic Pathways Involved in the Growth and Quality of Water Dropwort [Oenanthe javanica (Blume) DC.] under Nutrient Solution Culture. Plants, 12(7), 1459.
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