The mean particle size and polydispersity index were assessed using dynamic light scattering (DLS, Zetasizer Nano ZS, Malvern Instruments Ltd., Worcestershire, UK). The electrical characteristics of the samples were determined using electrophoretic light scattering. Transmission electron microscopy (TEM, Hitachi High-Tech Corporation, Tokyo, Japan) was employed to observe the morphological characteristics of the NLC-Pip–BSA samples. Spectral characterization was achieved by Fourier-transform infrared spectroscopy (FTIR, Bruker Vertex 70, Etilingen, Germany) and fluorescence spectroscopy (FP-650 Spectrofluorometer Jasco, Tokyo, Japan). The entrapment efficiency was determined by UV-Vis spectroscopy (UV-Vis Spectrophotometer V670 Jasco, Tokyo, Japan) [31 (link),32 (link)]. In vitro activity of the prepared NLCs was studied using real-time cells assay (RTCA, ACEA Biosciences, San Diego, CA, USA), MTS assay (CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega, Madison, WI, USA), and flow cytometry(FACS Canto II cytometers, Becton Dickinson, Immunocytometry System, Mountain View, CA, USA) [33 (link),34 (link),35 (link),36 (link)]. For detailed characterization procedures see Supplementary Materials Section, S1.2.1. to S1.2.10.
Real time cell analyzer (rtca)
Manufactured by Agilent Technologies
Sourced in United States, China
The RTCA (Real-Time Cell Analysis) is a laboratory equipment product from Agilent Technologies. It is designed to monitor cell proliferation, migration, and adhesion in real-time. The RTCA utilizes electronic sensing technology to measure changes in electrical impedance, which provides quantitative information about the dynamic status of cultured cells.
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Lab products found in correlation
E plate, by Agilent Technologies (6 mentions)
Prism 7, by GraphPad (2 mentions)
Rtca software 2, by Agilent Technologies (2 mentions)
E plate 96, by Agilent Technologies (2 mentions)
Electronic cell counter, by Olympus (2 mentions)
Zetasizer nano z, by Malvern Panalytical (1 mentions)
Celltiter 96 aqueous one solution cell proliferation assay, by Promega (1 mentions)
Vertex 70, by Bruker (1 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)
Opti mem 1 reduced serum medium, by Thermo Fisher Scientific (1 mentions)
Matrigel, by BD (1 mentions)
Crystal violet, by Merck Group (1 mentions)
E plate 16, by Agilent Technologies (1 mentions)
Xcelligence system, by Roche (1 mentions)
Ez magna chip tma kit, by Merck Group (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Rtca 2, by Agilent Technologies (1 mentions)
E plate 96 pet, by Agilent Technologies (1 mentions)
Cck 8, by Merck Group (1 mentions)
Xcelligence system, by Agilent Technologies (1 mentions)
44 protocols using real time cell analyzer (rtca)
1
Comprehensive Nanoparticle Characterization for Pharmaceutical Applications
2
Cell Proliferation Assay for CRC Cells
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To evaluate cell proliferation ability, stable cell clones were plated at a density of 4 × 103 cells per well (ACT groups) or 9 × 103 cells per well (KO groups) of E‐plate 16 in a humidified atmosphere of 5% CO2 at 37°C. The proliferative indices of cells were recorded every 15 min until 60 h by using the real‐time cellular analysis (RTCA, ACEA Biosciences, USA) system, and cellular proliferative curves of each group were generated based on the mean values of quadruplicate wells. To determine the effect of LRP5 knockdown or activation on the sensitivity of CRC cells to cisplatin treatment, stable LRP5‐KO or LRP5‐ACT HCT‐116 cells and corresponding controls were seeded into the E‐plate 16 and incubated for 24 h, followed by treated with cisplatin at a concentration of 8 μg/ml for 36 h or 12 μg/ml for 24 h. The proliferation index was also recorded by using RTCA system. The inhibition rate of cisplatin on cells was calculated by the equation: [proliferation index (PBS)‐proliferation index (cisplatin)]/proliferation index (PBS) ×100%.
3
Resveratrol Impacts on Human Hair Cells
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Human hair dermal papilla cells (hDPCs) were obtained according to the previously described methods.27 (link) Cell proliferation was determined by Real Time Cellular Analysis (RTCA) (ACEA Biosciences, USA). A density of 8000 cells per well was seeded in a 16-well microplate. After incubation for 8 h, the medium was removed, followed by the addition of either the medium alone (Control) or the medium with varying concentrations of RSV (30, 35, 40, 50, 60, 125 and 250 μM, respectively).
4
Real-Time Cell Proliferation Assay
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The cell proliferation rate was assessed using a real-time cell analyzer (RTCA, ACEA Biosciences). Cells were suspended in the culture medium and 5000 cells were added into every well of the E-plate with 4 replicates. After incubation at room temperature for 15 mins, the E-plate was placed onto the RTCA Station in the incubator for continuous recording. Cell index values were recorded every 15 mins for a period of up to 120 hrs.
5
Real-Time Cell Proliferation Monitoring
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The xCELLigence experiments were performed using the RTCA (real-time cell analyzer) instrument, according to the manufacturers’ instructions (ACEA Biosciences, San Diego, CA, USA). The optimal seeding number was previously determined by cell titration and growth experiments (data not shown). The 2500 cells/well were then seeded and their proliferation was automatically monitored every 30 min and 24 h after seeding, cells were treated with DCA. The cell index was monitored up to 90 hours from seeding. Data were analyzed using xCELLigence software (Version 2.0, Acea biosciences, San Diego, CA, USA) and expressed as a mean ± SD of the cell index normalized to the last cell index recorded before the time of DCA addition.
6
Real-Time Cell Proliferation Assay
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Cell proliferation ability was determined using Real-Time Cellular Analysis (RTCA, ACEA Biosciences, USA). In brief, cells were seeded into E-plate 16 and incubated in 5% CO2 at 37°C. The proliferation index was recorded every 15 min to 54 h using RTCA software, and the cellular growth index was generated from the average values of six wells for each group.
7
Real-time Cellular Analysis of Proliferation
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Real-time cellular analysis (RTCA) (ACEA Biosciences, USA) was used to evaluate cell proliferation [16 (link)]. First, the baseline value was measured in 100 μl of culture medium preincubated at 37 °C in a cell incubator for 1 h. Then, the cells were seeded in wells at a density of 2000 cells per well. The attachment and proliferation of cells were measured by the RTCA system for 6 and 168 h, respectively.
8
Transfection and Proliferation Assay
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Normal human gastric mucosa epithelial cells and gastric cancer cells were transfected with pcDNA3.1-RMRP, empty vector, si-RMRP or si-NC using Lipofectamine 2000 reagent and Opti-MEM I Reduced Serum Medium (Life Technologies) in accordance with the manufacturer's instructions, respectively. Cell proliferation was analyzed with a real-time cell analyzer (RTCA; ACEA Biosciences, San Diego, CA) as previously reported [16 (link)].
9
Real-Time Cell Proliferation Monitoring
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Cell proliferation was detected by real time xCELLigence analysis system (RTCA) according to the manufacturer (ACEA Biosciences, CA, USA). After transfected with siRNA, expression plasmid or scramble control, respectively, 5 × 103 cells were seeded to each well of E-Plate and incubated at 37 °C with 5% CO2 with proliferation monitoring every 15 min for at least 90 h.
10
Cellular Migration and Invasion Monitoring
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RTCA (ACEA Biosciences, USA) was used to monitor cellular migration and invasion as previously described.23
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