Lgr6 egfp ires creert2

All mice work was approved by the CHB Animal Care and Use Committee, accredited by AAALAC, and was performed in accordance with relevant institutional and national guidelines and regulations. Lgr6-EGFP-IRES-CreERT2, Sftpc-CreERT2, Rosa26-lox-stop-lox-tdTomato, Rosa26-lox-stop-lox-YFP, and Rosa26-lox-stop-lox-DTR mice were purchased from Jackson Laboratories. The Scgb1a1-CreER^TM (Rawlins et al., 2009 (link)) and Lgr5-IRES-CreERT2 (Huch et al., 2013 (link)) mouse lines were kindly provided by Dr. Brigid Hogan and by Dr. Hans Clevers, respectively. Mice for the lineage tracing and injury experiments were on a C57BL/6 background and controls matched for sex and were littermates. 7-10 week old mice were used for most of experiments described in this study. Animal studies were reviewed and approved by the Massachusetts Institute of Technology (MIT) Committee for Animal Care (institutional animal welfare assurance no. A-3125-01) or the Boston Children’s Hospital’s Institutional Animal Care and Use Committee.

Lgr5-EGFP-IRES-CreER^T2 (Barker et al. 2007 (link)), Lgr6-EGFP-IRES-CreER^T2 (Snippert et al. 2010a (link)), Rosa-YFP (Srinivas et al. 2001 (link)), and Rosa-tdTomato (Madisen et al. 2010 (link)) mice were obtained from the Jackson Laboratory. K14CreER^T2 (Li et al. 2000 (link)) mice were provided by P. Chambon; Rosa-Confetti (Snippert et al. 2010b (link)) mice were provided by H. Clevers, K14rtTA transgenic mice (Nguyen et al. 2006 (link)) were provided by Elaine Fuchs, TetO-Cre mice (Perl et al. 2002 (link)) were provided by Andreas Nagy, and Sox9CreER^T2 mice (Kopp et al. 2011 (link)) were provided by Frédéric Lemaigre. The generation of K5CreER^T2 (Van Keymeulen et al. 2011 (link)), K19CreER^T (Means et al. 2008 (link)), and K8rtTA (Watson et al. 2015 (link)) mice was described previously. All experimental mice used for experiments on breasts were female, and all experimental mice used for prostate experiments were male. All animals were mixed strains. No statistical methods were used to predetermine sample size. The experiments were not randomized. The investigators were not blinded to allocation during experiments and outcome assessment. Mice colonies were maintained in a certified animal facility in accordance with European guidelines. The experiments were approved by the local ethical committee (Comission d'Ethique et du Bien-Etre Animal [CEBEA]).