Ammonium hydrogen carbonate

Manufactured by Merck Group

Sourced in United States, Germany

Ammonium hydrogen carbonate is a chemical compound with the formula (NH4)HCO3. It is a white, crystalline solid that is commonly used as a laboratory reagent.

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Lab products found in correlation

Formic acid, by Merck Group (8 mentions) Acetonitrile, by Merck Group (4 mentions) Acetic acid, by Merck Group (4 mentions) Trypsin, by Promega (4 mentions) Ammonium acetate, by Merck Group (3 mentions) Sodium hydroxide (naoh), by Merck Group (3 mentions) Iodoacetamide, by Merck Group (3 mentions) Dl dithiothreitol, by Merck Group (3 mentions) Milli q system, by Merck Group (2 mentions) Esi low concentration esi l tuning mix, by Agilent Technologies (2 mentions) Urea, by Merck Group (1 mentions) 2 5 dihydroxybenzoic acid, by Merck Group (1 mentions) 4 chloro α cyanocinnamicacid, by Merck Group (1 mentions) 1 4 dithiothreitol, by Carl Roth (1 mentions) Lc ms grade water, by Avantor (1 mentions) Lc ms grade methanol, by Thermo Fisher Scientific (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Methanol, by Thermo Fisher Scientific (1 mentions) Chlorogenic acid, by Merck Group (1 mentions) Apigenin 7 o glucoside, by Merck Group (1 mentions)

Close spelling variants of this product

Ammonium carbonate (86 citations) Ammonium hydrogen carbonate (NH4HCO3) (3 citations)

13 protocols using ammonium hydrogen carbonate

Hydroxyapatite-based Kefir Proteomics

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Hydroxyapatite
(CHT Ceramic
Type I, 40 μm) was purchased from Bio-Rad (Munich, Germany).
Formic acid (LC–MS grade), potassium chloride, 85% o-phosphoric
acid, ammonium hydrogen carbonate, 4-chloro-α-cyanocinnamic
acid, and 2,5-dihydroxybenzoic acid were obtained from Sigma-Aldrich
(Taufkirchen, Germany). Acetonitrile (LC–MS grade) was purchased
from Honeywell Fluka (Seelze, Germany) and 1,4-dithiothreitol was
purchased from Carl Roth (Karlsruhe, Germany). Tris(hydroxymethyl)aminomethane
hydrochloride and ammonia (25%) were purchased from Acros Organics
(Geel, Belgium) and urea was purchased from Merck Millipore (Darmstadt,
Germany). Alkaline phosphatase (1500 U, grade I) from calf intestine
was purchased from Roche Diagnostics (Mannheim, Germany). Purified
water was taken from a Milli-Q system (Merck Chemicals, Darmstadt,
Germany).
Three different kefir batches of the same brand were
purchased from a local supermarket. The kefir was industrially produced
from pasteurized and homogenized low-fat organic cow milk (1.5%) using
a complex culture of yeast and lactic acid bacteria.

Savastano M.L., Liu Y., Mels J., Dittrich D., Haus S., Gensberger-Reigl S, & Pischetsrieder M. (2019). Profiling of Multiphosphorylated Peptides in Kefir and Their Release During Simulated Gastrointestinal Digestion. ACS Omega, 4(5), 7963-7970.

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Optimized Sample Preparation for Mass Spectrometry

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All chemicals and reagents were obtained in the highest commercially available quality. 2,5-dihydroxybenzoic acid (DHB), trifluoroacetic acid (TFA) and ammonium hydrogen carbonate were obtained from Sigma-Aldrich (Steinheim, Germany), trypsin and methanol were purchased from Thermo scientific (Dreieich, Germany) and methanol (LC–MS grade) was purchased from Thermo Fisher Scientific (Geel, Belgium), Water (LC–MS grade) was purchased from VWR (Leuven, Belgium).

González de San Román E., Bidmon H.J., Malisic M., Susnea I., Küppers A., Hübbers R., Wree A., Nischwitz V., Amunts K, & Huesgen P.F. (2018). Molecular composition of the human primary visual cortex profiled by multimodal mass spectrometry imaging. Brain Structure & Function, 223(6), 2767-2783.

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Chrysanthemum Cultivars Phytochemical Analysis

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Six kinds of Chrysanthemum morifolium cultivars were purchased from different companies (Table 1). Apigenin-7-O-glucoside, chlorogenic acid, formic acid, acetonitrile, ammonium hydrogen carbonate, and ammonium acetate were obtained from Sigma-Aldrich (St Louis, MO, USA). Ultrapure water (18.2 MΩ cm) was further purified using a Milli-Q system (Millipore, Billerica, MA, USA). Unless otherwise mentioned, the chemicals and reagents used in this experiment were of analytical grade.

Wang Y., Xu Z., Wu Y., Li M., Pang S., Liang Z, & Ni Y. (2019). Effect of Weakly Basic Conditions on the Separation and Purification of Flavonoids and Glycosides from Chrysanthemum morifolium Tea. Molecules, 24(2), 297.

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Optimized Protein Extraction and Digestion

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All buffers and solutions
were prepared with analytical reagent grade chemicals. Acetic acid
(HAc, glacial), formic acid (HFor 98–100%), sodium hydroxide,
sodium citrate tribasic, and ammonia (25%) were provided by Merck
(Darmstadt, Germany). DL-Dithiothreitol (DTT, ≥99%), iodoacetamide
(IAA, ≥98%), ammonium acetate (≥99.9%), and ammonium
hydrogen carbonate (≥99.9%) were supplied by Sigma-Aldrich
(St. Louis, MO, USA). Propan-2-ol was purchased from Scharlab (Barcelona,
Spain), while acetonitrile and water were supplied by Sigma-Aldrich
(all of them were of LC-MS quality grade). Trypsin and Glu-C (both
sequencing grade modified) were provided by Promega (Madison, WI,
USA). The ESI low concentration (ESI-L) tuning mix for tuning and
calibration of the mass spectrometer was obtained from Agilent Technologies
(Waldbronn, Germany).

Mancera-Arteu M., Benavente F., Sanz-Nebot V, & Giménez E. (2023). Sensitive Analysis of Recombinant Human Erythropoietin Glycopeptides by On-Line Phenylboronic Acid Solid-Phase Extraction Capillary Electrophoresis Mass Spectrometry. Journal of Proteome Research, 22(3), 826-836.

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Mass Spectrometry Sample Preparation

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Formic acid (LC–MS grade), ammonium
hydrogen carbonate, bovine trypsin (sequencing grade), dithiothreitol
(DTT) (biochemistry grade), citrate dihydrate salt (biochemical grade),
and calcium dichloride were from Sigma Aldrich (St. Louis, United
States). Dichloromethane (DCM), acetonitrile (AcN) (HPLC and LC–MS
grade), methanol (MeOH) (HPLC synthesis or gradient grade), water
(LC–MS grade), and 2-propanol (2-PrOH) (LC–MS grade)
were from ChemLab (Bensheim, Germany). Trifluoroacetic acid (TFA),
Tris-(hydroxymethyl)-aminomethane (Tris), glycine, sodium chloride
(NaCl), acetic acid, and reduced or oxidized glutathione (GSH or GSSG,
respectively) were from Carl Roth (Karlsruhe, Germany). Endoprotease
ArgC was from Abnova (München, Germany).

Hellinger R., Muratspahić E., Devi S., Koehbach J., Vasileva M., Harvey P.J., Craik D.J., Gründemann C, & Gruber C.W. (2021). Importance of the Cyclic Cystine Knot Structural Motif for Immunosuppressive Effects of Cyclotides. ACS Chemical Biology, 16(11), 2373-2386.

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Glycoprotein Purification and Identification

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The IHNV glycoproteins were first isolated by DSA-agarose affinity followed by a second round of purification by immunoprecipitation with antibody against IHNV as described above. The viral glycoprotein interacting with zebrafish galectins was cut out for glycopeptide MS identification carried out as described elsewhere (Harrison et al. 2012 (link)). Briefly, gel bands were excised and destained using 50 mM ammonium hydrogen carbonate (Sigma-Aldrich) mixed with an equal amount of acetonitrile (Romil). The samples in the gel pieces were reduced and carboxymethylated by dithiothreitol (Roche Applied Science) and iodoacetic acid (Sigma-Aldrich). They were then digested by sequencing grade modified trypsin (Promega) and the supernatant, which contained (glyco) peptides, was transferred to a new tube. The (glyco) peptides remaining in the gel were extracted using 0.1% (v/v) trifluoroacetic acid (Romil), followed by acetonitrile. The supernatants were combined and concentrated for mass spectrometric analysis. Online nanoLC-MS was run on an API QSTAR Pulsar Hybrid LC/MS/MS system (Applied Biosystems/MDS Sciex). The data were analyzed using Analyst QS Software (Applied Biosystems).

Nita-Lazar M., Mancini J., Feng C., González-Montalbán N., Ravindran C., Jackson S., de las Heras-Sánchez A., Giomarelli B., Ahmed H., Haslam S.M., Wu G., Dell A., Ammayappan A., Vakharia V.N, & Vasta G.R. (2015). The zebrafish galectins Drgal1-L2 and Drgal3-L1 bind in vitro to the infectious hematopoietic necrosis virus (IHNV) glycoprotein and reduce viral adhesion to fish epithelial cells. Developmental and comparative immunology, 55, 241-252.

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Identification of Meat Species

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Water, acetonitrile, formic acid and methanol LC–MS grade were purchased from Sigma-Aldrich (Schnelldorf, Germany). Ammonium hydrogen carbonate, dithiothreitol (DTT), iodoacetamide (IAA) and all other chemicals were of molecular biology grade and obtained from Sigma-Aldrich. Sequence-grade modified trypsin was bought from Promega GmbH (Mannheim, Germany). Meat samples of cattle, horse and pig (longissimus muscle) and four different types of sausages (S1–S4), labelled as ‘horse sausages’ (coarsely minced, cooked, smoked and semi-dried) were purchased locally. Samples of about 5 g or 5 cm length were cut from raw or processed products and kept at − 80 °C until further protein analysis. Proteins derived from pig (Sus scrofa), horse (Equus caballus) and cattle (Bos taurus) were examined in the present study.

Montowska M, & Spychaj A. (2018). Quantification of species-specific meat proteins in cooked and smoked sausages using infusion mass spectrometry. Journal of Food Science and Technology, 55(12), 4984-4993.

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Lipid Synthesis and Characterization

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Methanol, acetonitrile, methyl‐tert‐butyl ether (MTBE), dichloromethane, palmitoyl chloride, heptadecanoyl chloride, stearoyl chloride, oleoyl chloride, 4‐dimethylamino pyridine, ammonium hydrogen carbonate, ammonium formate, 2‐propanol, formic acid, and acetic acid were purchased from Sigma–Aldrich (St Louis, MO, USA), and pyridine was purchased from VWR (Radnor, PA, USA). Deionized water was obtained from a MilliQ Gradient A10 system (Millipore, Billerica, MA, USA).
1,2‐Di‐(9Z‐octadecenoyl)‐sn‐glycero‐3‐phosphoethanolamine‐N‐nonadecanoyl (PE 18:1/18:1‐N‐19:0) and 1,2‐dioleoyl‐sn‐glycero‐3‐phosphoethanolamine‐N‐arachidonoyl (ammonium salt) (PE 18:1/18:1‐N‐20:4) were purchased from Avanti Polar Lipids (Alabaster, AL, USA) and l‐α‐phosphatidylethanolamine from Larodan Fine Chemicals AB (Malmö, Sweden). Lipid standard stock solutions at concentrations of 1 mmol/L were prepared in chloroform/Methanol (1:1, v/v).

Triebl A., Weissengruber S., Trötzmüller M., Lankmayr E, & Köfeler H. (2016). Quantitative analysis of N‐acylphosphatidylethanolamine molecular species in rat brain using solid‐phase extraction combined with reversed‐phase chromatography and tandem mass spectrometry. Journal of Separation Science, 39(13), 2474-2480.

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Protein Sample Preparation for Mass Spectrometry

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Each secretome sample was processed, by preparing a solution of 1 μg/μL of proteins and 40 mM of ammonium hydrogen carbonate (Sigma-Aldrich, USA). Reduction was obtained by adding 5 mM dithiothreitol to each sample, with an incubation of 20 min at 80°C. Finally alkylation was obtained by adding 10 mM iodoacetamide and incubation for 30 min at 37°C. Digestion was performed incubating the samples overnight with 0.25 mg/mL of trypsin solution at 37°C (substrate/enzyme = 100/1).

Rocchiccioli S., Cecchettini A., Ucciferri N., Terreni M., Viglione F., Trivella M.G., Citti L., Parodi O, & Pelosi G. (2015). Site-Specific Secretome Map Evidences VSMC-Related Markers of Coronary Atherosclerosis Grade and Extent in the Hypercholesterolemic Swine. Disease Markers, 2015, 465242.

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Synthesis and Characterization of Functionalized Gold Nanoparticles

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Elix Water Purification system (Merck Millipore, Molsheim, France) was used to obtain the high purity water (R ≥ 15 MΩ cm⁻¹) to prepare all the aqueous solutions. Spherical GNPs (colloidal stock solutions, 10 nm core size, 2.99 × 10¹⁴ NPs mL⁻¹) with various surface coatings (methoxyl-PEG2000-SH-GNPs, carboxyl-PEG3000-SH-GNPs, biotin-PEG5000-GNPs) were purchased from Cytodiagnostics (Burlington, ON, Canada). Cisplatin (cis-diammineplatinum (II) dichloride, ≥99.9%), silver nitrate, tricine, sodium phosphate monobasic, ammonium hydrogen carbonate, Trisma^® base (2-amino-2-(hydroxymethyl)-1,3-propanediol), with purity ≥99%, and sodium phosphate dibasic (≥98,5%) were purchased from Sigma–Aldrich (St. Louis, MO, USA). Sodium hydroxide (HPLC purity) and germanium ICP standard solution (1 g L⁻¹) were purchased from Supelco (Bellefonte, PA, USA), and acetic acid (HPLC purity) from Sigma–Aldrich (Darmstadt, Germany). Methanol (LC-MS grade) was purchased from POCH (Gliwice, Poland). Gases (argon and oxygen) of purity ≥99.999% (Messer, Bad Soden, Germany) were used for CRC in ICP-MS/MS.

Wróblewska A.M., Milewska A., Drozd M, & Matczuk M. (2022). Targeted Delivery of Cisplatin by Gold Nanoparticles: The Influence of Nanocarrier Surface Modification Type on the Efficiency of Drug Binding Examined by CE-ICP-MS/MS. International Journal of Molecular Sciences, 23(4), 2324.

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