Log In Sign up
The largest database of trusted experimental protocols

Veritas 96 well microplate luminometer

Manufactured by Promega
Visit Supplier
Sourced in United States

The Veritas™ 96-well Microplate Luminometer is a compact and automated instrument designed for luminescence-based assays. It measures light output from microplate wells, providing quantitative data for a variety of cell-based and biochemical applications.

Automatically generated - may contain errors

Lab products found in correlation

Substrate dispenser, by Promega (2 mentions) Nfat luc, by Promega (2 mentions) Creb1 luc, by Promega (2 mentions) Gata4 luc, by Promega (2 mentions) Tk luc, by Promega (2 mentions) Mef2 luc, by Addgene (2 mentions) Xfect reagent, by Takara Bio (2 mentions) Dual luciferase assay kit, by Promega (2 mentions) Passive lysis buffer (plb), by Promega (2 mentions) Dual luciferase reporter assay protocol, by Promega (1 mentions) Lipofectamine 3000 reagent, by Thermo Fisher Scientific (1 mentions) Dual luciferase reporter assay kit, by Promega (1 mentions)

Close spelling variants of this product

Veritas 96-well luminometer 96-well microplate Luminometer Veritas Microplate Luminometer 96 Microplate Luminometer Veritas luminometer Microplate luminometer Veritas

4 protocols using veritas 96 well microplate luminometer

1

Dual-Luciferase Assay for miRNA Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Luciferase activity was assessed according to the Dual-Luciferase Reporter Assay protocol (Promega, Madison, WI) using a Veritas™ 96-well Microplate Luminometer (Promega, Madison, WI) with substrate dispenser (Promega, Madison, WI). HEK293T cells transduced with leti-miR-424 or control virus were seeded in 96-well plates with 70% confluence. 12 hours later, the cells were cotransfected with 50 ng pGL3-Promoter -UTR and 10 ng pRLTK using the Lipofectamine LTX. After 24 hours of transfection, the cells were harvested for firefly and Renilla luciferase activity assay. The renilla luciferase activities were used to normalize the transfection efficiency.
Yang H., Zheng W., Shuai X., Chang R.M., Yu L., Fang F, & Yang L.Y. (2015). MicroRNA-424 inhibits Akt3/E2F3 axis and tumor growth in hepatocellular carcinoma. Oncotarget, 6(29), 27736-27750.
+ Open protocol
+ Expand
2

Luciferase Assay for miR-379-3p Targeting

Check if the same lab product or an alternative is used in the 5 most similar protocols
To generate the luciferase construct, the circPOLQ and IL-10 genes containing the miR-379–3 p binding site were synthesized by GeneChem (China) and inserted into the GV272 vector. Next, PMA-treated THP-1 cells were treated with IL-10-3′-UTR-Luc firefly luciferase constructs (wild-type or mutant) using Lipofectamine 3000 reagent (Invitrogen, USA). The Luc firefly luciferase construct (wild-type or mutant) was co-transfected with an miR-379–3 p mimic. Finally, 48 hours after transfection, the cell lysates were collected, and the firefly/Renilla luminescence was quantified using a Veritas 96-well microplate luminometer (Promega, USA) following the procedure of the Dual-Luciferase Reporter Assay Kit procedure (Biyuntian, China). Neutase activity was assessed using a substrate dispenser from Promega (USA). Renilla luciferase activity was normalized to firefly luciferase activity.
Sun Z., Xu Y., Shao B., Dang P., Hu S., Sun H., Chen C., Wang C., Liu J., Liu Y, & Hu J. (2024). Exosomal circPOLQ promotes macrophage M2 polarization via activating IL-10/STAT3 axis in a colorectal cancer model. Journal for Immunotherapy of Cancer, 12(5), e008491.
+ Open protocol
+ Expand
3

Transcription Factor Activation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNCMs were transfected with NFAT-luc, CREB1-luc, GATA4-luc, TK-luc (Promega) or MEF2-luc (Addgene), and using X-fect reagent (Clontech) according to manufacturer’s protocol. After 24 hours transfection, cells were stimulated with 25 μM of PE for 6 hours, lysates harvested using passive lysis buffer (Promega) and luciferase activity determined using the Dual Luciferase assay kit (Promega) and Veritas 96 well microplate luminometer (Turner Biosystems) following manufacturer’s protocol.
Lee D.I., Zhu G., Sasaki T., Cho G.S., Hamdani N., Holewinski R., Jo S.H., Danner T., Zhang M., Rainer P.P., Bedja D., Kirk J.A., Ranek M.J., Dostmann W.R., Kwon C., Margulies K.B., Van Eyk J.E., Paulus W.J., Takimoto E, & Kass D.A. (2015). Phosphodiesterase 9A Controls Nitric-oxide Independent cGMP and Hypertrophic Heart Disease. Nature, 519(7544), 472-476.
+ Open protocol
+ Expand
4

Transcription Factor Activation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNCMs were transfected with NFAT-luc, CREB1-luc, GATA4-luc, TK-luc (Promega) or MEF2-luc (Addgene), and using X-fect reagent (Clontech) according to manufacturer’s protocol. After 24 hours transfection, cells were stimulated with 25 μM of PE for 6 hours, lysates harvested using passive lysis buffer (Promega) and luciferase activity determined using the Dual Luciferase assay kit (Promega) and Veritas 96 well microplate luminometer (Turner Biosystems) following manufacturer’s protocol.
Lee D.I., Zhu G., Sasaki T., Cho G.S., Hamdani N., Holewinski R., Jo S.H., Danner T., Zhang M., Rainer P.P., Bedja D., Kirk J.A., Ranek M.J., Dostmann W.R., Kwon C., Margulies K.B., Van Eyk J.E., Paulus W.J., Takimoto E, & Kass D.A. (2015). Phosphodiesterase 9A Controls Nitric-oxide Independent cGMP and Hypertrophic Heart Disease. Nature, 519(7544), 472-476.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!