Ethylene glycol

NEB buffer 4 and Nt.BbvCI endonuclease were obtained from New England BioLabs (Ipswich, MA, USA), and the polymerase Klenow fragment exo- was purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Streptavidin-coated 96-well plates were purchased from BEAVER Nano-Technologies (Suzhou, China). 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonic acid (CHAPS), tris(2-carboxyethyl)phosphine hydrochloride (TCEP), Tween 20, and invertase from baker’s yeast were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ethylene glycol, bis(aminoethyl ether)-N, N, N’, N’ tetraacetic acid (EGTA), glycerol, sulfosuccinimidyl-4-(N-maleimidomethyl)-cyclohexane-1-carboxylate (sulfo-SMCC), bovine serum albumin (BSA), deoxynucleotide triphosphate (dNTP) solution mixture, 40% acrylamide mix solution, ammonium persulfate (APS), and 1,2-bis(dimethylamino)-ethane (TEMED) were obtained from Sangon Biotechnology Co. Ltd. (Shanghai, China). All other chemicals were of analytical grade and were used as received without purification. All water used in this work was RNase-free.
The oligonucleotides used in this assay (Table 1) were synthesized and purified by Sangon Biotechnology Co. Ltd. (Shanghai, China). Buffer I (0.1 M NaCl, 0.1 M sodium phosphate buffer, pH 7.3, 0.05% Tween-20) was used throughout the experiment.

Yeast extract and tryptone were purchased from Oxoid (Shanghai, China). Pyridoxal-5’-phosphate (PLP), pyruvate and R-1-phenylethylamine (1-(R)-PEA) were purchased from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Choline chloride (ChCl), urea, ethylene glycol (EG), propylene glycol (PG), glycerol, formate, kanamycin sulphate, isopropyl–β-d-thiogalactopyranoside (IPTG), sodium dihydrogen phosphate, disodium phosphate, imidazole, sodium chloride, Ni-NTA Sefinose™ Resin (Settled Resin), Modified Bradford Protein Assay kit and SDS PAGE gel kit were obtained from Sangon (Shanghai, China). Amicon^®® Ultra Filter units were obtained from Sigma Aldrich (Shanghai, China). All chemicals were of analytical grade. The transaminase genes from Aspergillus terreus, which were previously transformed in E. coli BL21 (DE3) pRSF-D-AAO competent cells, were stored in our laboratory at −80 °C. The plasmids and the E. coli BL21 (DE3) pRSF-D-AAO competent cells were constructed by Anhui General Biology Company (Anhui, China).