Navitoclax was synthesized at AbbVie, Inc. (North Chicago, IL). FDA-approved drug library was purchased from Enzo (Farmingdale, NY). Albendazole, mebendazole, oxibendazole, and oxfendazole were purchased from Sigma (St. Louis, MO). All siRNA pools were purchased from Dharmacon (Lafayette, CO). Noxa antibody was purchased from Abcam. Bim and Mcl-1 antibodies were purchased from Epitomics (Burlingame, CA). Caspase 3 and caspase 9 inhibitors were purchased from SantaCruz Biotechnology (Santa Cruz, CA). All the branched DNA reagents were purchased from Affymetrix (Santa Clara, CA).
Fda approved drug library
Manufactured by Enzo Life Sciences
The FDA-approved drug library is a comprehensive collection of FDA-approved small molecule drugs. This library serves as a valuable resource for researchers and scientists conducting drug discovery and development studies.
Automatically generated - may contain errors
Lab products found in correlation
Mebendazole, by Merck Group (1 mentions)
Oxibendazole, by Merck Group (1 mentions)
Oxfendazole, by Merck Group (1 mentions)
Navitoclax, by Abbvie (1 mentions)
Sirna pool, by Horizon Discovery (1 mentions)
Albendazole, by Merck Group (1 mentions)
384 well black low volume polypropylene plates, by Thermo Fisher Scientific (1 mentions)
Mantis liquid dispenser, by Formulatrix (1 mentions)
4 protocols using fda approved drug library
1
Navitoclax Drug Screening Protocol
2
Ciclopirox Protects RPE Cells from Oxidative Stress
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Example 1
Initial screening of a library with 640 FDA-approved drugs (Enzo Life Sciences FDA-approved drug library) with known bioactivity, safety & bioavailability, and natural products was performed using TBHP, a chemical oxidizing agent, as a model of oxidative stress-induced cell death. Ciclopirox olamine was identified as a compound that significantly protects ARPE-19 cells from TBHP-induced cell death and this model was used to explore the protective mechanisms of ciclopirox olamine on human RPE cells.
After exposure to TBHP for 24 hours, treatment with ciclopirox olamine protects ARPE-19 cells from oxidative stress-induced cell death (
3
Screening of 2366 Compounds
4
Screening FDA-Approved Drugs for SARS-CoV-2 Inhibitors
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For the screening, 40 nl of 10 mM compound stocks dissolved in DMSO from the FDA-approved drug library (Enzo Life Sciences) were transferred to 384-well black low-volume polypropylene plates (Fisherbrand). The sample mixture containing 1 μM CFP-SARS-CoV-2 nsp3 macrodomain and 5 μM MARylated YFP-GAP was prepared in assay buffer (10 mM Bis–Tris-Propane pH 7.0, 3%(w/v) PEG20,000, 0.01%(v/v) Triton X-100 and 0.5 mM TCEP) and 20 μl per well were dispensed using Mantis liquid dispenser (Formulatrix). The rFRET signal was determined after a 5-minute incubation time. The sample mixtures in presence or absence of 200 μM ADP-ribose were used as positive and negative controls, respectively.
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