Secondary horseradish peroxidase hrp conjugated anti mouse or anti rabbit igg antibodies

S. aureus (strain RN6390) was maintained in tryptic soy broth and agar plates (TSB/TSA; Sigma-Aldrich, St. Louis, MO). 2-Deoxy-glucose was purchased from Cayman Chemical (Ann Arbor, MI). For in vitro experiments cells were pretreated with 10mM of 2DG for 1 h followed by indicated infection times. For in vivo applications, the left eyes of each mouse was injected with 25 μg/eye (1μl volume) of 2DG, 14–16 h prior to or 6 h post bacterial infection. The dose of 2DG for intravitreal injection was chosen based on preliminary dose (10–100 μg/eye) titration. Contralateral eyes with sterile PBS injection were used as a controls. An ERK inhibitor, U1260, was purchased from InvivoGen (San Diego, CA) and cells were pretreated for 1 h with 10 μM of U1260. Antiphospho-ERK and ERK antibodies were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX). Anti-HSP90 antibody was purchased from Cell Signaling Technology (Beverly, MA). Secondary horseradish peroxidase (HRP)-conjugated anti-mouse or antirabbit IgG antibodies were purchased from Bio-Rad (Hercules, CA).