Euthanasia was performed with a mixture of ketamine/xylazine using a lethal intraperitoneal dose of 200 mg/kg of ketamine-16 mg/kg of xylazine. Whole blood sampling was performed by cardiac puncture and centrifuged at 2000 rpm for 15 min. Once the serum was separated from the blood clot, the samples were stored at −80 °C for later analysis. The thoracic aorta was removed and stored at −80 °C in a sterile tube with 1 mL of RNAlaterTM stabilizer solution (Ambion Inc., Austin, TX, USA).
Rnalater stabilizer solution
Manufactured by Thermo Fisher Scientific
Sourced in United States
RNAlaterTM stabilizer solution is a chemical reagent designed to rapidly permeate and stabilize RNA in biological samples. It prevents degradation of RNA, allowing for its efficient extraction and preservation for downstream analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rnalater stabilizer solution
1
Cardiac Blood Sampling and Aorta Collection
2
Serum and Tissue Sampling for Analysis
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Euthanasia was performed with a mixture of ketamine/xylazine with a lethal intraperitoneal dose of 200 mg/kg of ketamine-16 mg/kg of xylazine. A whole blood sample was collected by cardiac puncture and centrifuged at 2000 rpm for 15 min. Once serum was separated from the blood clot, the sample was stored at −80 °C for subsequent analysis. The thoracic artery was removed and stored at −80 °C in a sterile tube with a 1 mL RNAlaterTM stabilizer solution (Ambion Inc., Austin, TX, USA).
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