Log In Sign up
The largest database of trusted experimental protocols

Bicinchoninic acid bca protein assay kit

Manufactured by Meilun
Visit Supplier
Sourced in China

The Bicinchoninic acid (BCA) protein assay kit is a colorimetric detection and quantitation assay used for the determination of total protein concentration in a sample. The kit utilizes the bicinchoninic acid reaction to produce a purple-colored complex in proportion to the amount of protein present in the sample. This allows for the spectrophotometric quantitation of total protein concentration.

Automatically generated - may contain errors

Lab products found in correlation

Cell counting kit 8 cck 8, by Meilun (2 mentions) Trypsin, by Meilun (2 mentions) Dulbecco s modified eagle s medium dmem, by Meilun (1 mentions) Alizarin red s staining kit for osteogenesis, by Beyotime (1 mentions) Rpmi 1640 culture medium, by Solarbio (1 mentions) Easysep mouse t cell isolation kit, by STEMCELL (1 mentions) Fetal bovine serum (fbs), by Meilun (1 mentions) Dimethyl sulfoxide (dmso), by Solarbio (1 mentions) Annexin 5 fitc pi apoptosis detection kit, by Meilun (1 mentions) Penicillin, by Meilun (1 mentions) Streptomycin, by Meilun (1 mentions) Flow cytometry staining buffer, by MultiSciences Biotech (1 mentions) Alginate, by Meilun (1 mentions) Gelatin, by Meilun (1 mentions) Phosphate buffered saline (pbs), by Meilun (1 mentions) Disulfiram, by Merck Group (1 mentions) Anti β actin antibody, by Beyotime (1 mentions) Hif 1α ab1, by Abcam (1 mentions) Gsdmd antibody, by Affinity Biosciences (1 mentions) Total glutathione peroxidase gpx assay kit with nadph, by Beyotime (1 mentions)

4 protocols using bicinchoninic acid bca protein assay kit

1

Nanohydroxyapatite Cytotoxicity and Immunomodulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
The nHA (purity >97%, CAS 1306-06-5) was purchased from Aladdin Industrial Inc. (Shanghai, China). Cell Counting Kit-8 (CCK-8), Annexin V-FITC/PI apoptosis detection kit, bicinchoninic acid (BCA) protein assay kit, Dulbecco’s modified Eagle’s medium (DMEM), trypsin, fetal bovine serum (FBS), penicillin and streptomycin were supplied by Meilunbio (Dalian, China). EasySep™ mouse T cell isolation kit was obtained from StemCell Technologies (Vancouver, CA, United States). Anti-mouse CD3 (Clone 17A2), anti-mouse CD28 (Clone 37.51) were purchased from Peprotech (Westlake Village, CA, United States). Anti-mouse CD4, phycoerythrin (PE, Clone GK1.5), Anti-mouse CD8α, phycoerythrin-cyanine 7 (PE-Cy7, Clone 53-6.7) and flow cytometry staining buffer was purchased from Multi Sciences Biotech, Co., Ltd. (Hangzhou, China). 5-(and-6)-Carboxyfluorescein diacetate, succinimidyl ester (CFSE) was obtained from Abbkine Bioadvisers. Alizarin red S (ARS) staining kit for osteogenesis was purchased from Beyotime (Shanghai, China). Dimethyl sulfoxide (DMSO) and RPMI 1640 culture medium were obtained from Solarbio (Beijing, China). Deionized (DI) water was used in all experiments. All chemicals were used without additional purification.
Guo F., Yuan C., Huang H., Deng X., Bian Z., Wang D., Dou K., Mei L, & Zhou Q. (2022). Regulation of T Cell Responses by Nano-Hydroxyapatite to Mediate the Osteogenesis. Frontiers in Bioengineering and Biotechnology, 10, 884291.
+ Open protocol
+ Expand
2

Bioactive Scaffold for Bone Regeneration

Check if the same lab product or an alternative is used in the 5 most similar protocols
All reagents including alginate (Mw = 600 Kg/mol, M/G = 1.2, the viscosity is 200 mPa-s), gelatin and CaCl2, NaOH, phosphate-buffered saline (PBS), Bicinchoninic Acid (BCA) Protein Assay Kit (Meilunbio), and all cell culture reagents were available from Solarbio. Aladddin’s Ce(NO3)3·6H2O was employed as the starting compound. Cell culture investigations were conducted using MC3T3-E1. Jiancheng Bioengineering Institute provided the Alizarin red stain (ARS) and alkaline phosphatase (ALP) assay kit.
Li F., Li J., Song X., Sun T., Mi L., Liu J., Xia X., Bai N, & Li X. (2022). Alginate/Gelatin Hydrogel Scaffold Containing nCeO2 as a Potential Osteogenic Nanomaterial for Bone Tissue Engineering. International Journal of Nanomedicine, 17, 6561-6578.
+ Open protocol
+ Expand
3

Comprehensive Protein Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bicinchoninic acid (BCA) protein assay kit (#MA0082-1) and RIPA lysis buffer (#MA0153) were purchased from Meilunbio (Dalian, China). L-arginine (L-Arg) monohydrochloride (#A6969), Collagenase IV (#C9407), Disulfiram (#HY-B0240) and DAPI (#D9542) were from Sigma-Aldrich (St. Louis, MO, USA) of Merck. GSDMD antibody (#AF4012) was from Affinity (Shanghai, China), REDD1 (Regulated in Development and DNA Damage Responses 1). Antibody (#sc-376671) and p-NF-Kb (p-P65) (#sc-398442) were from Santa Cruz Biotechnology (CA, USA). Resveratrol (#A2122398) was from Aladdin (Shanghai, China). TXNIP(#ab188865) and HIF-1α (#ab1) were from Abcam (Boston, MA, USA). Malondialdehyde (MDA) assay kit (#S0131S), catalase (CAT) (#S0051) assay kit, total glutathione peroxidase (GPX) assay kit with NADPH (#S0058) and anti-β-actin antibody (AF0003) were purchased from Beyotime Biotech (Shanghai, China). Mouse IL-1β ELISA kit (E-EL-M0037c) was purchased from Elabscience Biotechnology Co. Ltd. (Wuhan, China). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) of Merck.
Zhang C., Niu H., Wan C., Yu X., Xin G., Zhu Y., Wei Z., Li F., Wang Y., Zhang K., Li S., Dong Y., Li Y, & Huang W. (2022). Drug D, a Diosgenin Derive, Inhibits L-Arginine-Induced Acute Pancreatitis through Meditating GSDMD in the Endoplasmic Reticulum via the TXNIP/HIF-1α Pathway. Nutrients, 14(13), 2591.
+ Open protocol
+ Expand
4

Murine Macrophage Activation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
RPMI-1640, penicillin streptomycin, trypsin, bicinchoninic acid (BCA) protein assay kit and cell counting kit-8 (CCK-8) were purchased from Meilunbio (Dalian, China). Fetal bovine serum (FBS) was purchased from ExCell Bio (Shanghai, China). Lipopolysaccharide and 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindodicarbocyanine,4-chlorobenzenesulfonate salt (DiD) fluorochrome were purchased from Sigma-aldrich. Granulocyte-macrophage colony-stimulating factor (GM-CSF), Interleukin (IL) -2, IL-4, IL-13, and interferon-γ (IFN-γ) were purchased from Peprotech (USA). Dibenzocycolctyne-cyanine5 (DBCO-Cy5), N-azidoacetylmannosamine-tetraacylated (Ac4ManNAz) and dibenzylcyclooctyne- NHS ester (DBCO-NHS) were purchased from Macklin (Shanghai, China). Goat Anti-Rat IgG H&L (FITC) and anti-SOCS1 antibody were purchased from Abcam. Type II bovine collagen, Freund’s complete adjuvant and Freund’s incomplete adjuvant were purchased from Chondrex (Washington, USA).
Lin M., Lei S., Chai Y., Xu J., Wang Y., Wu C., Jiang H., Yuan S., Wang J., Lyu J., Lu M, & Deng J. (2024). Immunosuppressive microvesicles-mimetic derived from tolerant dendritic cells to target T-lymphocytes for inflammation diseases therapy. Journal of Nanobiotechnology, 22, 201.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!