Go6983

Manufactured by Selleck Chemicals

Sourced in United States

Go6983 is a lab equipment product. It is a chemical compound used for research and development purposes. The core function of Go6983 is to serve as a research tool for scientific investigations.

Automatically generated - may contain errors

Lab products found in correlation

U73122, by Selleck Chemicals (2 mentions) Penicillin, by Thermo Fisher Scientific (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) Berberine hydrochloride, by Meilun (1 mentions) Rabbit polyclonal antibodies against erk1 2, by Santa Cruz Biotechnology (1 mentions) P creb, by Santa Cruz Biotechnology (1 mentions) Quinolinic acid, by Merck Group (1 mentions) Oligomycin, by Merck Group (1 mentions) Forskolin, by Merck Group (1 mentions) Calyculin a, by Abcam (1 mentions) Aicar, by Merck Group (1 mentions) Human egf, by Cell Signaling Technology (1 mentions) Non targeting control pool d 001810 10 20, by Horizon Discovery (1 mentions) Antimycin a, by Merck Group (1 mentions) γ 32p atp, by PerkinElmer (1 mentions) Phorbol 12 myristate 13 acetate pma, by Abcam (1 mentions) Jnk in 8, by Selleck Chemicals (1 mentions) Ipatasertib, by Selleck Chemicals (1 mentions) Cyclosporin a, by Selleck Chemicals (1 mentions) R0 31 8220, by Selleck Chemicals (1 mentions)

14 protocols using go6983

Berberine Hydrochloride Signaling Pathway

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Berberine hydrochloride (purity ≥98%) was purchased from Dalian Meilun Biotechnology Co., Ltd. and dissolved in double‐distilled water. Quinolinic acid was purchased from Sigma‐Aldrich. Go6983 (a pan‐PKC inhibitor) was purchased from Selleck Chemicals. The rabbit polyclonal antibodies against GSK3β, p‐GSK3β(S9) and p‐PKCζ(T410) were purchased from Bioworld. The rabbit polyclonal antibodies against PKCζ were purchased from Proteintech. The rabbit polyclonal antibodies against ERK1/2, p‐ERK1/2, CREB, p‐CREB and BDNF, along with the mouse polyclonal antibodies against β‐actin and secondary antibodies, were purchased from Santa Cruz.

Liu P., Li Y., Qi X., Xu J., Liu D., Ji X., Chi T., Liu H, & Zou L. (2019). Protein kinase C is involved in the neuroprotective effect of berberine against intrastriatal injection of quinolinic acid‐induced biochemical alteration in mice. Journal of Cellular and Molecular Medicine, 23(9), 6343-6354.

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Signaling Pathway Modulation Assay

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[γ-³²P]ATP was purchased from PerkinElmer. HG-10-102-01, MLi-2, GSK2578215A, LRRK2-IN1, MRT67307, GDC0941, AG1478, BID1870, H-89, PD0325901, UO1216, Rapamycin and Phos-tag acrylamide were all synthesized by Natalia Shpiro (University of Dundee). Phos-tag acrylamide stocks are stored in aliquots at −80°C and working stocks are stored for a few weeks at 5 mM aqueous solution at 4°C in opaque tubes given the photo-sensitive nature of this reagent. iN04 was purchased from ChemBridge Corporation (#7989904). Full-length recombinant human LRRK2[G2019S] was purchased from Invitrogen (#A15202). GZD-824 was purchased from Cayman Chemical (#21508) and calyculin A (#ab141784) was purchased from Abcam, Inc. Phorbol 12-myristate 13-acetate (PMA) (#P8139), interleukin-1A (#I2778), oligomycin (#75351), antimycin A (#A8674), AICAR (#A9978) and forskolin (#F6886) were all purchased from Sigma–Aldrich. Human EGF (#8916), and human IGF-1 (#3093) were purchased from cell signaling technology (CST). PKC-specific inhibitor GO6983 was purchased from Selleckchem (#S2911). ON-TARGETplus SMARTpool human LRRK1 (#L-005320-00-0005), human LRRK2 (#L-049666-00-0005) and non-targeting control pool (#D-001810-10-20) siRNA were purchased from Horizon Discovery.

Malik A.U., Karapetsas A., Nirujogi R.S., Mathea S., Chatterjee D., Pal P., Lis P., Taylor M., Purlyte E., Gourlay R., Dorward M., Weidlich S., Toth R., Polinski N.K., Knapp S., Tonelli F, & Alessi D.R. (2021). Deciphering the LRRK code: LRRK1 and LRRK2 phosphorylate distinct Rab proteins and are regulated by diverse mechanisms. Biochemical Journal, 478(3), 553-578.

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Elucidating Splicing Regulation by TCR Signaling

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To elucidate the roles of signal transduction proteins to regulate splicing changes, each protein known to be regulated downstream of TCR signaling was targeted by at least two different inhibitors. Human primary CD4+ CD45RO- T cells were incubated with individual inhibitors for 1 hr and stimulated with bound anti-CD3 and soluble anti-CD28 antibodies for 48 hr. Cells were then harvested for RNA purification and low-cycle RT-PCR analysis for splicing quantifications. The inhibitors utilized are as follows: PCKi: R0-31-8220 (Selleckchem: S7207) and Go6983 (Selleckchem: S2911), p38i: SB20350 (Selleckchem: S1076) and Skepinone-L (Selleckchem: S7214), NFATi: Cyclosporin A (Selleckchem: S2286) and FK506 (Selleckchem: S5003), AKTi: MK-2206 (Selleckchem:S1078) and Ipatasertib (Selleckchem:S2808), JNKi: SP600215 (Selleckchem: S1460) and JNK-IN-8 (Selleckchem: S4901) and Tanzisertib (Selleckchem: S8490).

Blake D., Radens C.M., Ferretti M.B., Gazzara M.R, & Lynch K.W. (2022). Alternative splicing of apoptosis genes promotes human T cell survival. eLife, 11, e80953.

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Optimizing Inhibitor Concentrations for Immune Cell Studies

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MCC950 (Adipogen, San Diego, CA), glycine (Fisher Scientific, Waltham, MA) and potassium chloride (Fisher Scientific) were resuspended in deionized water. Go6983 (Selleck Chemicals, Houston, TX), Ac-Tyr-Val-Ala-Asp-chloromethylketone (Ac-YVAD-CMK or YVAD) (Cayman Chemical, Ann Arbor, MI), MI2 (Tocris Bioscience, Bristol, UK), PS1145 (Cayman Chemical), R406, and entospletinib (Selleck Chemicals), were all resuspended in DMSO. Monocytes were treated with the inhibitors or with an equivalent volume of the appropriate vehicle, for 40 min at 37°C and then infected or stimulated as described above. MCC950, Go6983, MI2, PS1145, R406 and entospletinib were all added to infected cells in half log titrations to determine the concentrations of the inhibitors that did not induce cell death or reduce infection efficiency.

Pandori W.J., Lima T.S., Mallya S., Kao T.H., Gov L, & Lodoen M.B. (2019). Toxoplasma gondii activates a Syk-CARD9-NF-κB signaling axis and gasdermin D-independent release of IL-1β during infection of primary human monocytes. PLoS Pathogens, 15(8), e1007923.

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Pharmacological Characterization of Dopamine Receptor Modulators

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SGC‐CBP30, SKF83566, SCH23390, and SKF38393 were purchased from Tocris Bioscience; Y‐27632 dihydrochloride, GF109203, and GO6983 were from Selleck Chemicals; heparin and DAPI were purchased from Sigma; GloSensor cAMP Reagent and CellTiter‐Glo were from Promega.

Wang Q., Dong X., Lu J., Hu T, & Pei G. (2020). Constitutive activity of a G protein‐coupled receptor, DRD1, contributes to human cerebral organoid formation. Stem Cells (Dayton, Ohio), 38(5), 653-665.

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Cell Line DAN (CRL-2130) Culture and Treatment

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The DAN (CRL-2130) cell line was purchased from the ATCC (Manassas, VA, USA) and cultured in RPMI1640 medium supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific, Shanghai, China) and 100 IU/ml penicillin and 100 μg/ml streptomycin (Thermo Fisher Scientific). The cells were treated with 10 nM Go6983 (Selleckchem, Texas, USA), 10 nM bisindolylmaleimide IX (Bis IX) (Sigma-Aldrich), or the Akti-1/2 inhibitor (Abcam, Waltham, MA, USA) for indicated times to cell harvest and analysis.

Hu H.J., Deng X.W., Li R.X., Chen D.W, & Xue C. (2018). Inhibition of protein kinase C activity inhibits osteosarcoma metastasis. Archives of Medical Science : AMS, 15(4), 1028-1034.

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Inhibition of PKC Signaling Pathway

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Go 6983, a PKC inhibitor, was purchased from Selleck and dissolved in DMSO. The final DMSO concentration of the solution used throughout the study did not exceed 0.1%. Cells were grown to 70–80% confluence on plates and treated with 1 μM of Go 6983 for 12 h. Then the cells were digested with trypsin and used in the following experiments.

Hou J., Wang Z., Xu H., Yang L., Yu X., Yang Z., Deng Y., Meng J., Feng Y., Guo X, & Yang G. (2015). Stanniocalicin 2 Suppresses Breast Cancer Cell Migration and Invasion via the PKC/Claudin-1-Mediated Signaling. PLoS ONE, 10(4), e0122179.

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Selective CREB and Pan-PKC Inhibition in MDA-MB-231 Cells

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A selective CREB inhibitor, 666-15 (HY-101120, MedChemExpress), was purchased and used in our experimental design to treat MDA-MB-231 cells at a concentration of 73 nM. A pan-PKC inhibitor (Go 6983, Selleck) was purchased and used to treat MDA-MB-231 cells at a concentration of 5 μM.

Qin Y., Chen W., Jiang G., Zhou L., Yang X., Li H., He X., Wang H.L., Zhou Y.B., Huang S, & Liu S. (2020). Interfering MSN-NONO complex–activated CREB signaling serves as a therapeutic strategy for triple-negative breast cancer. Science Advances, 6(8), eaaw9960.

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Immortalized Mouse Podocyte Culture

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Immortalized mouse podocytes were a generous gift of Professor Ai Hua Zhang from Children's Hospital of Nanjing Medical University; the cells were cultured in RPMI 1640 medium with 10% foetal bovine serum (FBS) at 33 °C as described previously 24 (link). Podocytes were thermo-shifted to 37 °C for 10 days before they were used in the experiments. Chemical agents, GLPG0974 (10 μmol/L) (R&D Systems, USA), AICAR (10 μmol/L), U73122 (10 μmol/L), and Go 6983 (10 μmol/L) (Selleck, USA), were acquired for the experiments.

Lu J., Chen P.P., Zhang J.X., Li X.Q., Wang G.H., Yuan B.Y., Huang S.J., Liu X.Q., Jiang T.T., Wang M.Y., Liu W.T., Ruan X.Z., Liu B.C, & Ma K.L. (2021). GPR43 deficiency protects against podocyte insulin resistance in diabetic nephropathy through the restoration of AMPKα activity. Theranostics, 11(10), 4728-4742.

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Synthesis and Characterization of Ricefield Eel Peptide

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Ricefield eel Ist was synthesized by ChinaPeptides Co., Ltd., (Shanghai, China). The purity of synthesized peptide is more than 95% (analyzed by HPLC) and its structure was verified by mass spectrometry. Rp-cAMPS was purchased from Santa Cruz (TX, USA), and Go6983 and U73122 from Selleckchem (TX, USA). All the other chemicals were obtained from Sigma Chemical Co. (St. Louis, MO, USA). The above reagents were carefully diluted in accordance with the respective manufacturer’s instructions to prepare the stock solution and stored at −80°C.

Yang W., Zhang N., Shi B., Zhang S., Zhang L, & Zhang W. (2018). Isotocin Regulates Growth Hormone but Not Prolactin Release From the Pituitary of Ricefield Eels. Frontiers in Endocrinology, 9, 166.

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