Female New Zealand white rabbits (1.5–2.0 kg) were purchased from Charles River Laboratories (Wilmington, MA). RPTCs were isolated via the iron oxide perfusion method, and RPTCs were cultured under improved conditions as described previously48 (link), 49 (link). Three days after initial playing, dedifferentiated RPTCs were trypsinized and replated on XF-96 polystyrene culture microplates (Seahorse Bioscience, North Billerica, MA) at a density of 18,000 cells/well and were maintained at 37 °C for 3 days before pharmacological manipulation. For other RPTC experiments, isolated renal proximal tubules were plated in 35 mm dishes and used at confluence 6 days after initial plating. All experiments were carried out in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All procedures were approved by the Institutional Animal Care and Use Committees of the Medical University of South Carolina and the University of Arizona, and appropriate efforts were made to reduce animal suffering.
Female new zealand white rabbits
Manufactured by Charles River Laboratories
Sourced in United States, Germany
Female New Zealand white rabbits are a common laboratory animal model used in a variety of research applications. They are a robust and well-characterized breed that provides a consistent and reliable test subject.
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Lab products found in correlation
Addavax, by InvivoGen (1 mentions)
Incomplete freund s adjuvant, by Merck Group (1 mentions)
Complete freund s adjuvant, by Merck Group (1 mentions)
Growth factor supplement additives, by Cell Biologics (1 mentions)
M1168, by Cell Biologics (1 mentions)
Fetal bovine serum (fbs), by Cell Biologics (1 mentions)
Easy strainer 100 μm, by Greiner (1 mentions)
Hartley guinea pigs, by Charles River Laboratories (1 mentions)
Golden syrian hamsters, by Charles River Laboratories (1 mentions)
Ifnar, by Jackson ImmunoResearch (1 mentions)
C57bl 6j, by Jackson ImmunoResearch (1 mentions)
Confoscan 4, by Nidek (1 mentions)
Tono pen xl, by Medtronic (1 mentions)
25 protocols using female new zealand white rabbits
1
Isolation and Culture of Renal Proximal Tubular Cells
2
Rabbit Immunization for Serum Collection
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Female New Zealand White rabbits were purchased from Charles River (Sulzfeld, Germany) and maintained in the animal facilities of the Innsbruck Medical University. Groups of 4 animals were immunized intramuscularly at weeks 0, 3, and 6 with 2 × 108 TCID50. Prior to the first immunization and 3 weeks after each immunization blood was collected. Blood was allowed to coagulate for 1 h at room temperature and serum was prepared afterwards. Serum was stored at −80 °C.
3
Rabbit Immunization with Recombinant Proteins
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Female New Zealand white rabbits (Charles River labs) were immunized twice intra-muscularly at 14-day intervals with 50 μg of purified recombinant proteins mixed with Emulsigen adjuvant (MVP Adjuvants). Two animals were used per immunogen. All animal experiments were approved by the U.S. FDA Institutional Animal Care and Use Committee (IACUC) under Protocol #2008-10. The animal care and use protocol meets National Institutes of Health guidelines. Sera were collected before (pre-vaccination) and 8 days after the first and second vaccination and analyzed for binding antibodies in ELISA, SPR, neutralization assay and GFPDL analysis.
4
Intradermal mRNA-LNP Vaccination in Rabbits
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Female New Zealand white rabbits aged 6 weeks were obtained from Charles River Laboratories. Animals were induced with butorphanol (0.2 mg/kg) and dexmedetomidine (0.02 mg/kg) subcutaneously, masked with isoflurane, and then shaved on their backs and injected intradermally with mRNA-LNPs diluted in PBS. Six sites of injection (45 μL each) were used. Animals were randomly designated to experimental groups. After injections, the rabbits were reversed with atipamezole (0.2 mg/kg) subcutaneously and monitored until fully recovered.
5
Smoke Exposure and Antidepressant Effects
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All animal studies were performed with the approval of the Institutional Animal Care and Use Committee of Columbia University. Rabbits were smoke exposed as previously described.8 Briefly, female New Zealand White Rabbits (Charles River Laboratories, Wilmington, MA) were smoke exposed 4 hours per day, 5 days per week for a total of 20 weeks with total particulate matter (TPM) maintained at 100–150 g/m3. At week 10, a cohort of animals received oral duloxetine (10 mg/kg) for the remainder of the smoke exposure period. C57BL/6 mice were chronically exposed to smoke in a TE‐10 Teague Smoking Apparatus (Teague Enterprise). Eight‐week‐old mice were smoke exposed for 5 hours a day, 5 days a week for 10 days. The TPM within the smoking chamber was regulated so that the mice receive a TPM of 100–150 mg/m3. TPM was determined by a gravimetric analysis of filter samples taken daily during the exposure period. After 6 days of smoke, the mice were treated twice per day with oral gavage of fluoxetine at a concentration of 10 mg/kg body weight.
6
New Zealand White Rabbit Housing
7
Rabbit and Human Heart Tissue Protocol
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The use of rabbits for this study was approved by the Animal Care and Use Committee Mittelfranken, Bavaria, Germany. Female New Zealand White rabbits (2.5–3.0 kg) were purchased at Charles River Germany. Animals were sedated with ketamine/xylazine i.m. (25 mg/kg and 5 mg/kg) and killed by i.v. injection of pentobarbital (200 mg/kg). Immediately afterwards, the heart was removed.
Human tissue samples were obtained from the Cardiac Transplant Program (University of Utah Health Science Center). The institutional review board of the institution approved the study, and all patients provided informed consent.
Human tissue samples were obtained from the Cardiac Transplant Program (University of Utah Health Science Center). The institutional review board of the institution approved the study, and all patients provided informed consent.
8
NTC5 Recombinant Protein Vaccine Immunization
9
Rabbit Welfare in NIH Research
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All rabbit studies were reviewed and approved under protocol LMIV 1E by the Institutional Animal Care and Use Committee (IACUC) at the National Institutes of Health (approval code: LMIV 1E and approval date: 1 November 2021) and performed in an American Association for Accreditation of Laboratory Animal Care (AAALAC)-accredited facility (AAALAC file #000777, last accredited in 2021). The PHS Animal Welfare Assurance (File Number # D16-00602) was last approved 30 May 2023.
Female New Zealand White rabbits approximately 11 weeks of age and weighing between 2.2 and 2.6 kg were obtained from Charles River Laboratories, pair-housed in 6-cage racks from Allentown, Inc. (Allentown, NJ, USA). Health monitoring was performed twice daily and rabbits were fed Rabbit Chow LabDiet® 5321 (Richmond, IN, USA), given water ad libitum, with enrichment given 2–3 times per week. Studies were designed to reduce animal numbers where possible, and no more than momentary pain or distress was anticipated. All housing, husbandry practices, and pain management were in accordance with AAALAC guidelines, standards, and regulations.
Female New Zealand White rabbits approximately 11 weeks of age and weighing between 2.2 and 2.6 kg were obtained from Charles River Laboratories, pair-housed in 6-cage racks from Allentown, Inc. (Allentown, NJ, USA). Health monitoring was performed twice daily and rabbits were fed Rabbit Chow LabDiet® 5321 (Richmond, IN, USA), given water ad libitum, with enrichment given 2–3 times per week. Studies were designed to reduce animal numbers where possible, and no more than momentary pain or distress was anticipated. All housing, husbandry practices, and pain management were in accordance with AAALAC guidelines, standards, and regulations.
10
Rabbit Immunization with Viral Antigen
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Female New Zealand white rabbits from Charles River Laboratories (Wilmington, MA, USA) received injections of viral antigen on days 0, 30, and 60 followed by exsanguination on day 90. The day 0 injection consisted of 250 µL of antigen emulsified with 250 µL of complete Freund’s adjuvant (Sigma-Aldrich). The remaining injections consisted of 250 µL of antigen emulsified with 250 µL of incomplete Freund’s adjuvant (Sigma-Aldrich). The injected virus antigen was clarified supernatant centrifuged at 2000 RPM at 4°C for 15 min. The minimum pre-inactivation or live titer for injected viral antigen was 1.0 × 106 TCID50/mL.
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