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4 protocols using anti ng2 antibody sc 166251

1

Antibody Characterization for Cell Analysis

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The anti-NG2 antibody (sc-166251) and CK2β antibody (E9) were from Santa Cruz Biotechnology. The anti-α-tubulin (B-5-1-2/T5168) antibody was from Sigma-Aldrich. The anti-Akt1/2/3 antibody (11E7) was from Cell Signaling (Frankfurt am Main, Germany). The anti-CK2α #26 and anti-CK2α’ #30 antibodies were generated as described previously [26 (link)]. The human anti-CD31 antibody and anti-AKT1 (phospho S129) antibody [EPR6150] was from Abcam (Cambridge, UK). The mouse anti-CD31 antibody (DIA310) was from Dianova (Hamburg, Germany). The peroxidase-labeled anti-rabbit antibody (NIF 824) and peroxidase-labeled anti-mouse antibody (NIF 825) were from GE healthcare (Freiburg, Germany). The flow cytometry antibodies anti-CD29 (555443), anti-activated CD29 (556049), anti-CD31 (555446), anti-chondroitin sulfate proteoglycan 4 (NG2) (562415) and IgG1-κ isotype control (555749) were from BD Biosciences (Heidelberg, Germany). The BrdU antibody (BU20A) was from eBioscience™ Fisher Scientific (Schwerte, Germany).
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2

Antibody-based Analysis of Signaling Pathways

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Anti-NG2 antibody (sc-166251) and anti-CK2β antibody (E9) were from Santa Cruz Biotechnology. Anti-β-actin antibody (66009) and anti-α-tubulin antibody (66031) were from Proteintech Germany GMBH (St. Leon-Rot, Germany). The anti-Akt1/2/3 antibody (11E7), anti-FAK antibody (3285) and anti-pFAK antibody (3283) were from Cell Signaling (Frankfurt am Main, Germany). Anti-CK2α antibody and anti-CK2α’ antibody were generated as described previously [50 (link)]. Anti-pAKT antibody (EPR6150) was from Abcam (Cambridge, UK). Peroxidase-labeled anti-mouse antibody (NIF 825) and peroxidase-labeled anti-rabbit antibody (NIF 824) were from GE healthcare (Freiburg, Germany). Anti-chondroitin sulfate proteoglycan 4 (NG2) (562415) was from BD Biosciences (Heidelberg, Germany) and the BrdU antibody (BU20A) was from eBioscience Fisher Scientific (Schwerte, Germany).
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3

Immunoblotting Antibody Validation

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The anti-p65 antibody (sc-372), the anti-PDGFR-β antibody (sc-432), the anti-β-actin antibody (sc-517582) and the anti-NG2 antibody (sc-166251) were obtained from Santa Cruz Inc. The antibodies anti-CD54 (ICAM-1) (555511), anti-CD62E (E-selectin) (551145), anti-CD106 (VCAM-1) (555647) and IgG1-κ Isotype Control (555749) were purchased from BD Biosciences (Heidelberg, Germany). Peroxidase-labeled anti-rabbit antibody (NIF 824) and peroxidase-labeled anti-mouse antibody (NIF 825) were obtained from GE Healthcare (Freiburg, Germany). The anti-HO-1 antibody (ADI-SPA-895) was from Enzo Life Sciences (Lörrach, Germany). The anti-α-tubulin antibody (ab56676), the anti-myeloperoxidase (MPO) antibody (ab9535), the anti-ICAM-1 antibody (ab124760), the anti-8-OHDG antibody (ab10802), the anti-eNOS antibody (ab5589), the anti-VCAM-1 antibody (ab134047), the anti-CD68 antibody (ab1252212) and the secondary biotinylated goat anti-rabbit antibody (ab64256) were purchased from Abcam.
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4

Antibody Characterization for Cell Analysis

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The anti-NG2 antibody (sc-166251) and the anti-CK2β antibody (E9) were from Santa Cruz Biotechnology (Heidelberg, Germany). The anti-α-tubulin antibody (66031) was from Proteintech Germany GmbH (St. Leon-Rot, Germany). The anti-AKT1/2/3 antibodies (11E7) and poly (ADP-ribose) polymerase (PARP) (9542) were from Cell Signaling (Frankfurt am Main, Germany). The anti-pAKTS129 antibody (ab133458) and the anti-NG2 antibody (ab129051) were from Abcam (Cambridge, UK). The anti-CD31 and anti-vimentin antibodies were from Dako Agilent (Hamburg, Germany). The anti-CK2α and anti-CK2β antibodies were generated, as described previously [29 (link)]. The peroxidase-labeled anti-rabbit antibody (NIF 824) and the peroxidase-labeled anti-mouse antibody (NIF 825) were from GE healthcare (Freiburg, Germany). The anti-chondroitin sulfate proteoglycan 4 (NG2; 562415) was from BD Biosciences (Heidelberg, Germany). The anti-5-bromo-2′-deoxyuridine (BrdU) antibody was from eBioscience (Thermo Fisher Scientific, Karlsruhe, Germany).
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