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KYSE410 is a laboratory equipment designed for cell culture applications. It is a cell line derived from a human esophageal squamous cell carcinoma. The core function of KYSE410 is to serve as a research tool for studies related to esophageal cancer.

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Lab products found in correlation

Kyse30, by Japanese Collection of Research Bioresources Cell Bank (2 mentions) Kyse510, by Japanese Collection of Research Bioresources Cell Bank (2 mentions) Kyse270, by Japanese Collection of Research Bioresources Cell Bank (2 mentions) Kyse140, by Japanese Collection of Research Bioresources Cell Bank (2 mentions) Kyse450, by Japanese Collection of Research Bioresources Cell Bank (2 mentions) Kyse180, by Japanese Collection of Research Bioresources Cell Bank (2 mentions) Kyse70, by Japanese Collection of Research Bioresources Cell Bank (1 mentions) Kyse150, by Japanese Collection of Research Bioresources Cell Bank (1 mentions) Kyse1440, by Japanese Collection of Research Bioresources Cell Bank (1 mentions) Rpmi 1640, by Merck Group (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Harvard Bioscience (1 mentions) Kyse170, by Japanese Collection of Research Bioresources Cell Bank (1 mentions) Kyse220, by Japanese Collection of Research Bioresources Cell Bank (1 mentions) Imr 32, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)

3 protocols using kyse410

1

Culturing and Characterizing Esophageal Squamous Cell Carcinoma Lines

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A total of 21 ESCC cell lines, specifically KYSE30, KYSE70, KYSE140, KYSE150, KYSE180, KYSE270, KYSE410, KYSE450, KYSE510, KYSE590, KYSE890, KYSE1170, KYSE1260, KYSE1440, NUEC2, TE1, TE2, TE3, WSSC, TT, and TTn, along with Het‐1A, a non‐cancerous epithelial cell line, were purchased from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan), the American Type Culture Collection (Manassas, VA, USA), or were established at Nagoya University.
5 (link) The cells were cultured in a humidified incubator at 37°C with an atmosphere composition of 5% CO2 using RPMI‐1640 (Sigma‐Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum and antibiotics.
24 (link)
Shinozuka T., Kanda M., Sato Y., Shimizu D., Tanaka C., Umeda S., Inokawa Y., Hattori N., Hayashi M., Nakayama G, & Kodera Y. (2023). Increased STX3 transcript and protein levels were associated with poor prognosis in two independent cohorts of esophageal squamous cell carcinoma patients. Cancer Medicine, 12(24), 22185-22195.
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2

Ultrasound Treatment of Esophageal Cancer Cells

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Human esophageal squamous cell carcinoma cell lines (KYSE-410, catalog number: JCRB1419; KYSE-1140, catalog number: JCRB1449) were purchased from Japanese Collection of Research Bioresources (JCRB) Cell Bank. Human Umbilical Vein Endothelial Cells (HUVECs) were provided by Shanghai Institutes for Biological Science (Shanghai, China). All these cell lines were incubated as a monolayer in 100-mm dishes in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen, Carlsbad, CA) with 10% fetal bovine serum (FBS; ScienCell, Carlsbad, CA, USA) and 1% penicillin/streptomycin (Biochrom) at 37°C in 5% CO2. About 6 × 105 cells were plated in a 25-cm2 flask about one day in advance of ultrasound exposure. Then, the microbubbles were added, and cells were instantly treated with ultrasound and then cultured for 24 h. Following these treatments, cells were collected for assays as described below. A volume of 0.6 mL per sample at a density of 2 × 106 cells/mL (culture passages 3 to 6) was prepared for the following US treatment.
Shi J., Fu C., Su X., Feng S, & Wang S. (2021). Ultrasound-Stimulated Microbubbles Inhibit Aggressive Phenotypes and Promotes Radiosensitivity of esophageal squamous cell carcinoma. Bioengineered, 12(1), 3000-3013.
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3

Esophageal and Neuroblastoma Cell Lines Cultivation

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Nine human ESCC cell lines, KYSE30, KYSE140, KYSE170, KYSE180, KYSE220, KYSE270, KYSE410, KYSE450, and KYSE510, were obtained from the Japanese Collection of Research Bioresources (JCRB) Cell Bank [22 (link)]. Two neuroblastoma cell lines, IMR-32 and KELLY, were obtained from the JCRB Cell Bank and Public Health England, respectively. KYSE140 was cultured in Ham's F12 medium containing 2% (v/v) FBS; KYSE30, KYSE170, KYSE180, KYSE220, KYSE270, KYSE410, KYSE450, and KYSE510 were cultured in Ham's F12/RPMI1640 medium containing 2% (v/v) FBS; IMR-32 was cultured in MEM medium containing 10% (v/v) FBS and non-essential amino acid (NEAA); and KELLY was cultured in RPMI1640 medium containing 10% (v/v) FBS.
Nakazato H., Takeshima H., Kishino T., Kubo E., Hattori N., Nakajima T., Yamashita S., Igaki H., Tachimori Y., Kuniyoshi Y, & Ushijima T. (2016). Early-Stage Induction of SWI/SNF Mutations during Esophageal Squamous Cell Carcinogenesis. PLoS ONE, 11(1), e0147372.
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