Milliplex rat mouse igf 1 single plex assay

After 5 weeks on diet, blood was collected from mice fasted 4-6 h by retro-orbital bleed, allowed to clot for 30 m at room temperature, and serum was collected and flash frozen for subsequent analyses. Insulin and leptin were measured using mouse adipokine LINCOplex®Multiplex Assays (Millipore, Inc., Billerica, MA). Insulin-like growth factor 1 (IGF-1) concentrations were measured using a Millipore Milliplex Rat/Mouse IGF-1 Single Plex Assay. Adiponectin concentrations were measured using a Milliplex Mouse Adiponectin Single Plex (Millipore, Inc.). Serum concentrations of mouse cytokines, including IL-1β, IL-6, IL-10, IL-17A, interferon-γ (IFN-γ), TNF-α, and MCP-1, were measured using a Bio-Plex Pro™ Mouse Cytokine 7-Plex Assay (Bio-Rad, Inc., Hercules, CA). All assays were completed and analyzed on a Bio-Rad Bio-Plex 200 analysis system (Bio-Rad, Inc., Hercules, CA).

Serum was collected from mice fasted 6–8 hours, prior to tumor cell injection (week 24) by retro-orbital bleed. Serum hormones, adipokines, and cytokines, including leptin, adiponectin, insulin, and IL-6, were measured using mouse adipokine LINCOplex®Multiplex Assays (Millipore, Inc., Billerica, MA) and analyzed on a BioRad Bioplex 200 analysis system (Biorad, Inc., Hercules, CA). Insulin-like growth factor 1 (IGF-1) concentrations were measured using a Millipore Milliplex Rat/Mouse IGF-1 Single Plex assay (Millipore, Inc.).