Following treatment of ox-LDL for 24 h, ox-LDL-induced HUVECs were inoculated into six-well plates until 80% confluence. HUVECs were respectively transfected with miRNA inhibitor-negative control (NC; 5 nM; cat. no. miR2N0000001-1-5; Guangzhou RiboBio Co., Ltd.), miR-301a-3p inhibitor (5 nM; cat. no. miR20000688-1-5; Guangzhou RiboBio Co., Ltd.), miRNA mimic-NC (5 nM; cat. no. miR1N0000001-1-5; Guangzhou RiboBio Co., Ltd.), miR-301a-3p mimic (5 nM; cat. no. miR10000688-1-5; Guangzhou RiboBio Co., Ltd.), small interfering RNA (si) R-NC (5 nM; cat. no. siN0000002-1-5; Guangzhou RiboBio Co., Ltd.) and siKLF7 (5 nM; cat. no. siG000008609A-1-5; Guangzhou RiboBio Co., Ltd.) using Lipofectamine® 2000 transfection reagent (Invitrogen; Thermo Fisher Scientific, Inc.) for 48 h at 37˚C. Subsequent experiments were conducted 48 h after transfection.
Mirna mimic nc
Manufactured by RiboBio
Sourced in China
The MiRNA mimic NC is a laboratory product designed for use in miRNA research. It functions as a control for miRNA mimics, which are synthetic RNA molecules that mimic the activity of endogenous miRNAs. The core function of the MiRNA mimic NC is to serve as a reference or baseline in experiments involving miRNA mimics.
Automatically generated - may contain errors
Lab products found in correlation
Mirna inhibitor nc, by RiboBio (2 mentions)
Mir 301a 3p mimic, by RiboBio (1 mentions)
Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 3000 transfection reagent, by Thermo Fisher Scientific (1 mentions)
Mir 338 3p inhibitor, by RiboBio (1 mentions)
Mir 338 3p mimic, by RiboBio (1 mentions)
3 protocols using mirna mimic nc
1
Modulating miR-301a-3p in Ox-LDL Treated HUVECs
2
Osteogenic Differentiation of hBMSCs Using miR-619-5p
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hBMSCs were seeded in the 6-well plate at a density of 6×104, and the cells reached 70% confluence after about 48 h. According to the manufacturer’s instructions, miR-619-5p mimic and miRNA mimic NC (RiboBio, Guangzhou, China) were transfected with Lipofectamine 3000 transfection reagent (Invitrogen, US) at a concentration of 50 nM, and miR-619-5p inhibitor, miRNA inhibitor NC (RiboBio, Guangzhou, China) at a concentration of 100 nM. RNA was extracted 24 h after transfection, the protein was extracted 48 h for detection, and osteogenic induction solution was added for osteogenic induction differentiation. Transfection was performed again after 3 days. MiR-619-5p mimic and miR-619-5p inhibitor sequence are listed in Supplementary Table S5
3
MicroRNA Mimic and Inhibitor Transfection
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The miR-338-3p mimic, miR-338-3p inhibitor, miRNA mimic NC, and miRNA inhibitor NC were purchased from RiboBio (Guangzhou, China). Transfection was carried out using according to the manufacturer’s instructions.
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