Anti mdm2 antibody

Manufactured by R&D Systems

The Anti-MDM2 antibody is a laboratory tool used for the detection and analysis of the MDM2 protein. MDM2 is a protein that plays a critical role in the regulation of the tumor suppressor protein p53. This antibody can be used in various applications, such as Western blotting, immunohistochemistry, and immunoprecipitation, to study the expression and localization of MDM2 in different cell types and tissues.

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2 protocols using anti mdm2 antibody

Modulating MDM4 and p53 Acetylation

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Anti-MDM4 antibodies were purchased from Bethyl Laboratories. Anti-MDM2 antibody was purchased from R&D Systems. Anti–acetylated p53, anti-p53, anti–phospho ELK1, anti-ELK1, and anti–lamin B antibodies were from Cell Signaling. Anti-αSMA antibody was from American Research Products. Anti-Fas (CH11), anti-DD1α, anti-CD68, and anti–collagen I antibodies were from Thermo Fisher Scientific. Anti-AGE antibody was from Abcam. Anti-fibronectin and anti-GAPDH antibodies were from Santa Cruz Biotechnology. CXCL10 neutralizing antibody was from Thermo Fisher Scientific. CX3CL1 neutralizing antibody was from R&D Systems. The activities of commercial CXCL10 and CX3CL1 neutralizing antibodies were verified by functional blocking of cell chemotaxis. C646 and tamoxifen were from Sigma. CA was from MedChem Express. Validated MDM4 siRNAs were from OriGene.

Qu J., Yang S.Z., Zhu Y., Guo T., Thannickal V.J, & Zhou Y. (2021). Targeting mechanosensitive MDM4 promotes lung fibrosis resolution in aged mice. The Journal of Experimental Medicine, 218(5), e20202033.

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Fluorescent Mutant p53 Oligomerization

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The mutant p53 oligomerization domain peptide (mtp53ODP) called Cy5p53Tet was purchased from JPT peptide (Germany) at a purity >95%. The mtp53ODP is 35 amino acids long with an N-Terminal Cy5 fluorophore conjugation: H-CysCy5-RKKRRQRRGEYFTLQIRGRER-FEMFRELNEALELK-OH.
Solvents and reagents including dimethyl sulfoxide (DMSO), glutaraldehyde (GA), and anti-β-actin antibody (Cat# A2066) were obtained from Sigma-Aldrich. Anti-p53 antibody (DO-1, Cat# sc-126), anti-PARP1 antibody (Cat# sc-7150), and normal mouse IgG (Cat# sc-2025) were purchased from Santa Cruz. Magnetic beads were purchased from Cell Signaling. Anti-MDM2 antibody (Cat# AF1244) was obtained from the R&D System.

Xiao G., Annor G.K., Fung K., Keinänen O., Zeglis B.M, & Bargonetti J. (2020). Targeting Triple Negative Breast Cancer with a Nucleus-Directed p53 Tetramerization Domain Peptide. Molecular pharmaceutics, 18(1), 338-346.

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