Ab30443

Manufactured by Abcam

Sourced in United States

Ab30443 is a lab equipment product manufactured by Abcam. It is a general-purpose tool used in various research applications.

Automatically generated - may contain errors

Lab products found in correlation

Ab133448, by Abcam (1 mentions) Ab32047, by Abcam (1 mentions) Ex527, by Selleck Chemicals (1 mentions) P53 2524, by Cell Signaling Technology (1 mentions) Fk866, by Santa Cruz Biotechnology (1 mentions) Ab109210, by Abcam (1 mentions) Fitc goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) X tremegene hp, by Roche (1 mentions) Ab37259, by Abcam (1 mentions) Ab18822, by Abcam (1 mentions) Ab6313, by Abcam (1 mentions) Catch and release v2.0 reversible immunoprecipitation system, by Merck Group (1 mentions) Ab110025, by Abcam (1 mentions) Ab75852, by Abcam (1 mentions) Sc 32293, by Santa Cruz Biotechnology (1 mentions) P0161, by Agilent Technologies (1 mentions) Sc 13985, by Santa Cruz Biotechnology (1 mentions) Anti activated caspase 3, by Cell Signaling Technology (1 mentions) A5441, by Merck Group (1 mentions)

3 protocols using ab30443

Metformin, Berberine, and AMPK Pathway Modulation

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Metformin and berberine were purchased from MUSTBIO technology (Chengdu, China); Compound C was from CALBIOCHEM (Darmstadt, Germany). Hydroxychloroquine (HCQ), rapamycin, and phthalic acid (PHTH) were from Sigma‐Aldrich (CA, USA). Nicotinamide mononucleotide (NMN) and FK866 were from Santa Cruz Biotechnologies (TX, USA). EX527 was from selleck (CA, USA). Antibodies against LC3 (12741), phospho‐ACC (Ser79) (3661), phospho‐mTOR (Ser2448) (5536), phospho‐p70S6K (Thr389) (9206), and p53 (2524) were purchased from Cell Signaling technology (MA, USA). Those against phospho‐AMPKα (Thr172) (ab133448), Cathepsin B (ab30443), NAMPT (ab109210), AMPKα1 (ab32047), and SQSTM1/p62 (3340‐1) were from Abcam (MA, USA). Antibodies against β‐actin and Lamin B were from Proteintech (Beijing, China), anti‐TFEB (A303‐673A‐M) was from Bethyl (TX, USA), FITC‐goat anti‐rabbit IgG was from Invitrogen (CA). The reagent used for cDNA plasmids transfection was Lipofectamine 2000 (Invitrogen, CA), and that for lentivirus‐shRNA plasmids was X‐treme GENE HP (Roche, CA, USA).

Han X., Tai H., Wang X., Wang Z., Zhou J., Wei X., Ding Y., Gong H., Mo C., Zhang J., Qin J., Ma Y., Huang N., Xiang R, & Xiao H. (2016). AMPK activation protects cells from oxidative stress‐induced senescence via autophagic flux restoration and intracellular NAD + elevation. Aging Cell, 15(3), 416-427.

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Quantifying Cathepsins and Cystatin C in AAA

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To determine the levels of cathepsins B, D, K, L and S and cystatin C in the serum of AAA patients and the 10 healthy volunteers, immunoprecipitation was performed using the Catch and Release v2.0 Reversible Immunoprecipitation System, according to the manufacturer's instructions (Millipore Corporation, Billerica, MA, USA). The following Abs were used for detection: cathepsin B (ab30443, Abcam), cathepsin D (ab6313, Abcam), cathepsin K (ab37259, Abcam), cathepsin L (ab49984, Abcam), cathepsin S (ab18822, Abcam) and cystatin C (rabbit polyclonal to cystatin C; ab33487, Abcam). Following immunoprecipitation, the samples underwent western blot analysis as described above.

Lohoefer F., Reeps C., Lipp C., Rudelius M., Haertl F., Matevossian E., Zernecke A., Eckstein H.H, & Pelisek J. (2014). Quantitative expression and localization of cysteine and aspartic proteases in human abdominal aortic aneurysms. Experimental & Molecular Medicine, 46(5), e95-.

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Antibody-Based Profiling of Cell Death Pathways

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Primary antibodies used were mouse anti-LAMP1 (Abcam), anti-bovine serum albumin (ab79872, Abcam), anti-PDK1 (ab110025, Abcam), anti-cytochrome C (MAB897, BD Pharmingen), anti-β-actin (A-5441, Sigma), anti-α-tubulin (sc-32293, Santa Cruz), and rabbit anti-cathepsin B (EML-SA361, Enzo), anti-cathepsin B (ab30443, Abcam), anti-cathepsin B (sc-13985, Santa Cruz), anti-cathepsin D (ab75852, Abcam), anti-AIF (551429, BD Pharmingen), anti-endonuclease G (3035, ProSci), anti-activated caspase-3 (9661, Cell Signaling), anti-caspase-3 (9662, Cell Signaling). Peroxidase-conjugated secondary antibodies were as follows: rabbit anti-mouse (P0161, Dako), and goat anti-rabbit (12-348, Millipore Corporation) antibodies. Supplementary Materials and Methods are available.

Chien W.W., Niogret C., Jugé R., Lionnard L., Cornut-Thibaut A., Kucharczak J., Savina A., Salles G, & Aouacheria A. (2017). Unexpected cross-reactivity of anti-cathepsin B antibodies leads to uncertainties regarding the mechanism of action of anti-CD20 monoclonal antibody GA101. Leukemia research, 55.

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