Anti cox 2 and anti inos antibodies

The LPS (E. coli 026:B6), Griess reagent, 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT), epicatechin and berberine were obtained from Sigma (St. Louis, MO, USA). Enzyme linked immunesorbent assay (ELISA) kits for tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6 and IL-1β were acquired from Pierce Endogen (Thermo Scientific, Waltham, MA, USA). The PGE₂ ELISA kit was obtained from R&D Systems (Minneapolis, MN, USA). Anti-p-I-κBα, antibody and peroxidase-conjugated secondary antibody were purchased from Cell Signaling (Danvers, MA, USA). Anti-COX-2 and anti-iNOS antibodies were obtained from BD Biosciences (San Jose, CA, USA), while the anti-NF-κB, anti-I-κBα, anti-β-actin and anti-lamin A/C antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). All other chemicals were purchased from the Sigma Chemical Co. (St. Louis, MO, USA).

LPS (Escherichia coli 055:B5), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Tris base, glycine, NaCl, sodium dodecylsulphate (SDS), Griess reagent and bovine serum albumin (BSA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). RPMI 1640, foetal bovine serum (FBS) and antibiotic-antimycotic mixture were obtained from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Trypan blue vital stain (0.4%) was obtained from Life Technologies (Grand Island, NY, USA). PGE₂, TNF-α, IL-6 ELISA kits were obtained from R&D Systems (Minneapolis, MN, USA). Anti-COX-2 and anti-iNOS antibodies were obtained from BD Biosciences (San Diego, CA, USA). Anti-filaggrin, anti-serine palmitoyltransferase (SPT), anti-β-actin, and horseradish peroxidase (HRP)-conjugated secondary antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).