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4 protocols using hexanal

1

Comprehensive Analytical Standards Protocol

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The standards for gallic acid (GA), (+)–catechin (C), (−)–epicatechin (EC), (−)–epigallocatechin (EGC), (−)–gallocatechin gallate (GCG), (−)–epigallocatechin gallate (EGCG), (−)–epicatechin gallate (ECG), and caffeine were purchased from Sigma–Aldrich (Darmstadt, Germany). The mixture of amino acids standards was purchased from Sykam (Munich, Germany). n–Alkanes were purchased from Sigma–Aldrich (Darmstadt, Germany). Aroma standards including ethyl decanoate (99 %), benzaldehyde (99.5 %), octanal (99 %), benzyl alcohol (99.5 %), phenylacetaldehyde (95 %), linalool (98 %), nonanal (96 %), terpineol (95 %), geraniol (99 %), (Z)–3–hexenyl hexanoate (98 %), β–ionone (97 %), cedrol (98 %), and methyl jasmonate (98 %), were purchased from Aladdin (Shanghai, China). Aroma standards including hexanal (98 %), (Z)–3–hexenyl acetate (97 %), linalool oxide mixture (97 %), 2–phenylethyl alcohol (98 %), methyl salicylate (99 %), indole (99 %), (Z)–jasmone (92 %), α–ionone (90 %), nerolidol (97 %), and (Z)–3–hexenyl benzoate (98 %) were purchased from Tokyo Chemical Industry Co., ltd (Tokyo, Japan). Aroma standard ocimene (90 %) was purchased from Yuanye Company (Shanghai, China).
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2

Cultivation of Ganoderma Mushroom under Bamboo Forest

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S. rugosoannulata was obtained from the Hangzhou Academy of Agricultural Sciences, its name was Jinqiu 18, and the main cultivation substrate was 70.0% rice husk and 30.0% wood chips. It was cultivated under the moso bamboo (Phyllostachys edulis) in the Baiyan Temple forest Farm in Hangzhou. Altitude: 50 m, slope: 15–22°. The district has a warm, temperate, and continental monsoon climate characterized by hot and rainy summers and cold and dry winters. It has an annual mean temperature of 16.3 °C, 1467 h of sunshine, and 134 days of rain, with precipitation being 1460 mm. The average relative humidity is 78.3%. Another strain was cultivated in a greenhouse, with the temperature being 22–28 °C and the average relative humidity 75.0–90.0%.
Hexanal and 1-Hexanol (Analytical standard, ≥98.0%) were purchased from Tokyo Chemical Industry (Shanghai) Co., Ltd. (Shanghai, China). (E)-Nerolidol (Analytical standard, ≥98.0%) was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). 3-Octanone, (Z)-β-Farnesene, and 2-Undecanone (Analytical standard, ≥98.0%) were purchased from Shanghai Acmec Biochemical Co., Ltd. (Shanghai, China). Methanol (HPLC) and Ethanol (HPLC) were from Tedia Company, Inc. (Hangzhou, China).
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3

Synthetic Compounds Procurement for Research

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Synthetic chemical compounds identified by GC–MS were procured from different companies. Hexanal (98%) (CAS RN: 66-25-1), heptanal (95%) (CAS RN: 111-71-7), and trans-2-nonenal (95%) (CAS RN: 18829-56-6) were obtained from Tokyo chemical industry (Tokyo, Japan). Tetradecanal (96%) (CAS RN: 124-25-4) was purchased from Aladdin. n-Tetradecane (99.4%) (CAS RN: 629-59-4), n-pentadecane (99.5%) (CAS RN: 629-62-9), and n-heptadecane (99.8%) (CAS RN: 629-78-7) were acquired from Dr. Ehrenstorfer GmbH (Augsburg, Germany). 1-Dodecanol (99%) (CAS RN: 112-53-8) and 1-tetradecanol (98%) (CAS RN: 112-72-1) were purchased from ANPEL Laboratory Technologies Inc. (Shanghai, China). Furthermore, the cis-3-hexen-1-ol (CAS RN: 928-96-1), used as a reference control in the EAG tests, was purchased from Tokyo chemical industry (Tokyo, Japan).
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4

Isolation and Characterization of Heme Compounds

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Chlorohemin (heme) from porcine was purchased from Panreac Quimica SLU (Barcelona, Spain). ZnPP, protoporphyrin IX, adenosine 5'-triphosphate dipotassium salt dihydrate, tritridecanoylglycerol, pentadecanoic acid, and tridecanoic acid were purchased from Sigma-Aldrich (St. Louis, Missouri, United States). Hexanal was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). The methanol used was of HPLC grade (Merck KGaA, Darmstadt, Germany) and the other reagents used in this study were of ACS-grade.
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