Au2700 automatic biochemical analyzer

Manufactured by Olympus

Sourced in Japan

The AU2700 is an automatic biochemical analyzer designed for clinical laboratory use. It is capable of performing a variety of biochemical tests on patient samples to aid in medical diagnosis and treatment. The core function of the AU2700 is to automatically analyze and measure the levels of different biochemical compounds in a sample, providing healthcare professionals with valuable data to support their clinical decision-making.

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Close spelling variants of this product

AU2700 biochemical analyzer Automatic biochemical analyzer AU2700 analyzer Biochemical analyzer Automatic analyzer AU2700

22 protocols using au2700 automatic biochemical analyzer

Assessment of Blood Pressure, Uric Acid, and Kidney Function

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Blood pressure was measured in all participants by trained professionals using electronic sphygmomanometry. Blood pressure was measured in the right arm in the seated position with elbow and forearm resting on the armrest after the participant rested for 10 minutes^{31 (link),32 (link)}. Blood pressure was measured 2 times at 2-min intervals in all participants and mean systolic and diastolic blood pressure were recorded. Hypertension was defined as systolic blood pressure ≥ 140 mm Hg or diastolic blood pressure ≥ 90 mm Hg^{33 (link)}.
Venous blood samples were collected after an overnight fast (≥ 12 h). Serum uric acid concentration was measured by the uricaseperoxidase method^{34 (link)}. Hyperuricemia was defined as serum uric acid concentration ≥ 7 mg/dL in men or ≥ 6 mg/dL in women^{35 (link)}. The estimated glomerular filtration rate (eGFR) was measured using an Olympus AU2700 automatic biochemical analyzer. eGFR ≥ 60 mL/min/1.73 m² was regarded as conserved kidney function and eGFR < 60 mL/min/1.73 m² was regarded as reduced renal function^{36 (link)}. Total cholesterol, triglycerides, and fasting plasma glucose were measured using an Olympus AU2700 automatic biochemical analyzer.

Wang Y., Zhang W., Qian T., Sun H., Xu Q., Hou X., Hu W., Zhang G., Drummond G.R., Sobey C.G., Charchar F.J., Golledge J, & Yang G. (2021). Reduced renal function may explain the higher prevalence of hyperuricemia in older people. Scientific Reports, 11, 1302.

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Quantitative Analysis of Uric Acid in Biological Samples

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To demonstrate the application of the proposed method, the ratiometric fluorescent system comprising the N-CDs was utilized to quantitatively analyze UA in human serum and urine samples. Human blood samples provided by the Fourth Affiliated Hospital of Nanchang University (Nanchang, China) were centrifuged at 4000 rpm for 15 min, after which the supernatants (serum) were extracted and diluted 5-fold using PBS solution. To verify the results of the N-CDs-based fluorescence system, the human serum samples were also analyzed by an Olympus AU2700 automatic biochemical analyzer. Before biochemical analysis, the serum samples were centrifuged at 4000 rpm for 3 min. Each of the resulting supernatants (5.6 μL) was supplemented with aliquots of uricase, 4-aminoantipyrine (4-AAP), and 3-diphenylamine disodium salt (MADB). After that, the mixtures were analyzed by the biochemical analyzer at 660/800 nm.
The urine samples were collected from two volunteers in the morning and filtered through a 0.22-μm Millipore filter. The resulting filtrates were centrifuged at 8000 rpm for 5 min and diluted 50-fold with PBS solution for further use.

Li F., Rui J., Yan Z., Qiu P, & Tang X. (2021). A highly sensitive dual-read assay using nitrogen-doped carbon dots for the quantitation of uric acid in human serum and urine samples. Mikrochimica Acta, 188(9), 311.

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Plasma Lipid and Cytokine Profiling

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Blood sample was collected from the abdominal vena cava and the plasma supernatants were collected for further study. The levels of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), triglycerides (TG), and high-density lipoprotein cholesterol (HDL-C) were detected using an AU-2700 automatic biochemical analyzer (Olympus, Tokyo, Japan). The serum cytokine levels, including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), were detected using a Mcytomag-70 K-3 Mouse Cytokine/Chemokine Magnetic Bead Panel (Merck Millipore Co. LTD).

Huang Y., Ran X., Liu H., Luo M., Qin Y., Yan J., Li X, & Jia Y. (2023). A novel dammarane triterpenoid alleviates atherosclerosis by activating the LXRα pathway. Chinese Medicine, 18, 72.

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Measuring Serum Albumin and Prealbumin

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The levels of serum albumin and serum prealbumin were measured from fasting venous blood within 24 h after admission. Analyses were performed by Olympus AU2700 automatic biochemical Analyzer.

Wang B., Xin Y., Tang X., Wang F., Hua S., Yang Y., Xu S., Gong H., Dong R., Lin Y., Li C., Lin X, & Bi Y. (2024). Potential value of serum prealbumin and serum albumin in the identification of postoperative delirium in patients undergoing knee/hip replacement: an observational study and internal validation study. Frontiers in Neurology, 15, 1375383.

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Liver Function and Histology Assessment

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Activities of serum aspartate transaminase (AST) and alanine transaminase (ALT) were measured using an AU2700 automatic biochemical analyzer (Olympus, Tokyo, Japan). For histopathological analysis, liver tissue was fixed in 10% phosphate buffered formalin and paraffin embedded, and cut into 4 μm sections. Sections were stained with hematoxylin and eosin (H&E) and analyzed by light microscopy.

Yang X., Zhan Y., Sun Q., Xu X., Kong Y, & Zhang J. (2016). Adenosine 5′-monophosphate blocks acetaminophen toxicity by increasing ubiquitination-mediated ASK1 degradation. Oncotarget, 8(4), 6273-6282.

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Serum Biomarker Assessment of Renal Function

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Serum was centrifuged at 3600 g for 10 minutes and the supernatant was then used to assess the renal function. The levels of BUN and Scr were determined on an AU2700 automatic biochemical analyzer (Olympus, Japan). The levels of TNF-α, IL-1β and AGEs were measured according to the manufacturer’s instructions of the immunoassay kit. The assay employs the ELISA techniques.

Tang L., Li K., Zhang Y., Li H., Li A., Xu Y, & Wei B. (2020). Quercetin liposomes ameliorate streptozotocin-induced diabetic nephropathy in diabetic rats. Scientific Reports, 10, 2440.

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Streptozotocin-Induced Diabetic Kidney Hypertrophy

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Body weights and fasting blood glucose levels were recorded each week before and after STZ induction, including drug intervention period. FBG was measured by a blood glucose meter (LifeScan, CA, USA) in samples obtained from the tail vein. It was about 2 mins from blood collection to obtaining results. The renal hypertrophy index (mg/g) was calculated as follows: renal index = kidney weight (mg)/body weight (g). 24-h urine protein was determined on an AU2700 automatic biochemical analyzer (Olympus, Japan).

Tang L., Li K., Zhang Y., Li H., Li A., Xu Y, & Wei B. (2020). Quercetin liposomes ameliorate streptozotocin-induced diabetic nephropathy in diabetic rats. Scientific Reports, 10, 2440.

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Serum Biomarker Measurement Protocol

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Serum AST and ALT activities were measured using an AU2700 automatic biochemical analyzer (Olympus, Tokyo, Japan). Serum TNF-α, IL-1β and IL-6 levels were determined using enzyme-linked immunosorbent assay (ELISA) kits (Boster Biological Technology Ltd, Wuhan, China) according to the manufacturer's protocol.

Wang T., Wang Z., Yang P., Xia L., Zhou M., Wang S., Du J, & Zhang J. (2016). PER1 prevents excessive innate immune response during endotoxin-induced liver injury through regulation of macrophage recruitment in mice. Cell Death & Disease, 7(4), e2176-.

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Evaluating Diabetic Nephropathy Biomarkers

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The levels of FBG were measured before and after each STZ injection. Samples obtained from the tail vein were tested for FBG using a blood glucose meter (LifeScan, CA, United States). Metabolic cages were used to collect 24-h urine from rats. The levels of 24 h U-Alb, blood urea nitrogen (BUN), and serum creatinine (Scr) were measured using an AU2700 automatic biochemical analyzer (Olympus). AGE level was measured according to the ELISA kit’s instructions (Cusabio, China).

Tang L.X., Wei B., Jiang L.Y., Ying Y.Y., Li K., Chen T.X., Huang R.F., Shi M.J, & Xu H. (2022). Intercellular mitochondrial transfer as a means of revitalizing injured glomerular endothelial cells. World Journal of Stem Cells, 14(9), 729-743.

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Lipid Biomarker Measurement Protocol

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Fasting venous blood samples were collected to measure glucose, TC. HDL-C, triglyceride and creatinine levels using the Olympus AU2700 Automatic Biochemical Analyzer (Japan). LDL-C was calculated using the Friedewald equation. Hs-CRP was determined by the high-sensitivity nephelometric method. Plasma ox-LDL was measured by sandwich enzyme-linked immunosorbent assay procedure (Mercodia, Uppsala, Sweden)^{16 (link)}. The interassay coefficient of variation was 6.8%.
After centrifugation at 1500 × g for 10 min at 4 °C, plasma samples were stored at −80 °C for future measurements of Lp-PLA2 mass, as long-term stability of Lp-PLA2 has been demonstrated^{35 (link)}. Briefly, Lp-PLA2 mass was measured using a commercial enzyme-linked immunosorbent assay kit (Tianjin Kangerke Bioscience, Tianjin, China). The interassay coefficient of variation was 4.8%. All measurements were performed according to the manufacturers’ instructions.

Huang Y., Wu Y., Yang Y., Li W., Lu J, & Hu Y. (2017). Lipoprotein-associated phospholipase A2 and oxidized low-density lipoprotein in young patients with acute coronary syndrome in China. Scientific Reports, 7, 16092.

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