Turbo mass gold

GC-MS analysis of the ethyl acetate extracts found to be better than other solvents of Epaltes divaricata L. was performed using a perkin Elmer GC clarus 500 system comprising AOC-20i auto-sampler and a Gas chromatograph interfaced to a Mass spectrometer (GC-MS) equipped with a Elite-5MS (5% Diphenyl/95% Dimethyl Poly Siloxane) fused capillary column (30 × 0.25 μm 1D × 0.25 μm dF). For GC-MS detection, an electron ionization system was operated in electron impact mode with ionization system operated in electron impact mode with ionization energy of 70ev. Helium gas (99.999%) was used as carrier gas at a constant flow rate of 1 ml/min, and an injection volume of 2 μl was employed (split ratio of 10:1). The relative percentage amount of each component was calculated by comparing its average peak area to the total areas. The mass detector used in this analysis was Turbo-Mass Gold-Perkin Elmer and the software adopted to handle mass spectra and chromatograms was a Turbo-Mass ver 5.2. GC-MS was conducted using the database of central electrochemical research institute characterization and measurement laboratory having more than 62,000 patterns. The spectrum of the unknown components was compared with the spectrum of known components stored in the data bank, Central Electrochemical Research Institute Characterization and Sargam metals, Chennai, India.

Gas chromatography–mass spectrometry (GC-MS) analysis of the extracted sample was performed using an Agilent 5977B GC/MSD (Agilent, Santa Clara, CA, USA). Gas Chromatograph was equipped and coupled to a mass detector Turbo Mass Gold, PerkinElmer Turbo Mass 5.1 spectrometer with an Elite-1 (100% dimethylpolysiloxane), DB-5MS, 30 m × 0.25 mm i.d., 0.25 μm film thickness of capillary column. The instrument was set to an initial temperature of 80 °C and maintained at this temperature for 1 min. At the end of this duration, the oven temperature was raised to 300 °C, at the rate of an increase of 15 °C/min, and maintained for 9 min. The injection port temperature was ensured at 290 °C, and the helium flow rate was one mL/min. The ionization voltage was 70 eV. Samples were injected in split mode as 10:1. Mass spectral scan range was set at 30–600 (m/z) [30 (link)].

The AREO was obtained from the dried leaves of A. roseodora plant through conventional hydro-distillation in a Clevenger type apparatus following official method of European Pharmacopoeia (WorldCat, 2011 ). Chemical profiling of AREO was done through GC/MS (GC: Thermo scientific 1,300 GC and MS: Perkin Elmer Turbo mass Gold MA, United States) equipped with TG-5 capillary column (30 m × 0.25 mm ID × 0.25 μm thickness) and temperature of instrument was set from 60°C to 240°C with temperature rise at the rate of 5°C min⁻¹. The split ratio was kept 1: 50, helium was used as carrier gas, transfer line and oven temperatures were set according to standard protocols. The identification of components was done by comparing their kovats retention indices (KRI) and mass fragmentation pattern with those available in the literature (Adams, 2007 ). The KRI values of different components were calculated by using the retention times (RT) of a homologous series of n-alkanes (C₉–C₂₈ hydrocarbons, Polyscience Corp. Niles IL) running in parallel with EO.