For the cell apoptosis, the NP cells were washed three times. Seventy percent ethanol was applied to fix these cells and the cell density was adjusted to 1 × 106 cells. Next, 5 μl of Annexiv-FITC (Nanjing Genechem Co, Ltd., China) was added and incubated in darkness at 37 °C for approximately 30 min. Then, propidium iodide (PI) (Nanjing Genechem Co, Ltd., China) was added and continue to incubate in darkness for nearly 30 min. FC500 flow cytometry (Beckman, California, USA) was applied to test NP cell apoptosis.
Fc500 flow cytometry
The FC500 is a flow cytometry instrument manufactured by Beckman Coulter. It is designed to analyze and sort cells or other particles in a fluid sample, providing information about their size, granularity, and fluorescent properties. The FC500 utilizes laser technology to detect and measure the characteristics of individual cells or particles as they pass through a focused beam of light.
Lab products found in correlation
31 protocols using fc500 flow cytometry
Cell Cycle and Apoptosis Analysis of NP Cells
For the cell apoptosis, the NP cells were washed three times. Seventy percent ethanol was applied to fix these cells and the cell density was adjusted to 1 × 106 cells. Next, 5 μl of Annexiv-FITC (Nanjing Genechem Co, Ltd., China) was added and incubated in darkness at 37 °C for approximately 30 min. Then, propidium iodide (PI) (Nanjing Genechem Co, Ltd., China) was added and continue to incubate in darkness for nearly 30 min. FC500 flow cytometry (Beckman, California, USA) was applied to test NP cell apoptosis.
Lentiviral Transduction of MSCs
Cell Cycle and Apoptosis Analysis
Ang II and Candesartan Modulate AT1R in HCC
Cell Cycle and Apoptosis Analysis
A cell apoptosis assay was carried out by using Annexin V-FITC/propidium iodide (PI) Apoptosis Detection Kit (BD Biosciences, San Jose, CA, USA) according to the manufacturer’s recommendations. Briefly, U87 and U251 cells transfected with siRNA1-RACK1, siRNA2-RACK1, or si-NC were harvested after 72 h for apoptosis analysis. A total of 195 μL of binding buffer, 5 μL of Annexin V-FITC, and 3 μL of propidium iodide (PI) were added to the suspension, and cells were incubated for 10 min at room temperature in the dark. The cells were immediately examined by flow cytometry (Beckman Coulter, Atlanta, GA, USA). All experiments were conducted independently at least three times.
Cell Cycle and Apoptosis Analysis
Apoptosis Analysis of Metformin and Everolimus
Apoptosis Assay of ZC-14 Treatment
Characterization of Reprogrammed iPSCs
Quantifying M2 Macrophage Phenotypes
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