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St 40r

Manufactured by Thermo Fisher Scientific
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Sourced in Germany, United States

The ST 40R is a laboratory centrifuge designed for general-purpose applications. It features a fixed-angle rotor that can accommodate 40 tubes of up to 50 mL capacity. The centrifuge offers a maximum speed of 4,000 rpm and a maximum RCF of 3,000 x g. It is suitable for a variety of common laboratory procedures that require centrifugation.

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Lab products found in correlation

Optima grade methanol, by Thermo Fisher Scientific (1 mentions) Spd131dda speedvac concentrator, by Thermo Fisher Scientific (1 mentions) Nalgene rapid flow filter unit, by Thermo Fisher Scientific (1 mentions) Rotavapor r 300, by Büchi (1 mentions) Centrifuge 5810r, by Eppendorf (1 mentions) Multitron pro, by Infors (1 mentions)

16 protocols using st 40r

1

DHA Extraction from Microalgae Cells

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Example 2

A washed cell broth (250 g) containing microbial cells (Crypthecodinium cohnii) was pasteurized at 60° C. for 1 hour. The washed cells were lysed by passing the broth through a mechanical homogenizer (Microfluidizer M110) twice at 12,000 PSI. The lysed cell composition was placed in a flask and a demulsified lysed cell composition was formed by: (i) adding a 50% NaOH solution to adjust the pH to 7.5, adding 0.1%, by weight composition, Vinoflow® Max A(available from, Novozymes , Denmark); heating to 65° C.; and holding for 6 hours while agitating at a speed of 250 RPM, and then, while agitation continued, (ii) adding a 50% NaOH solution to adjust the pH to 10.5 and heating to 80° C. while agitating at a speed of 250 RPM until the pH dropped to 8.2. The microbial oil was separated from the demulsified lysed cell composition by centrifuging (Thermo Sorvall ST 40R) the composition at 8000 g for 5 minutes to provide a crude oil, which yielded 78% DHA (by DHA weight), based on FAME analysis and a crude oil with an AV of 6.4 and a PV of 0.42 meq.

US10364207B2. Processes for obtaining microbial oil from microbial cells (2019-07-30). DSM IP ASSETS B.V. [NL]. Inventors: Mark Barker [US], Nasrin Tabayehnejad [US], Ginger Shank [US], Neil Francis Leininger [US], Kirt Lyvell Matthews, Sr. [US].
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2

Optimizing Phragmites Biomass Pretreatment

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Biomass (20 grams of phragmites) was mixed well with 180 ml 0.5%, 1% and 2% (v/v) sulphuric acid in 500 ml screw-capped bottles, and soaked for 15 min before thermal treatment for 60 min at 121 °C (AMSCO Eagle Series 2041 Autocalve, Steris, Mentor, OH). After the pretreatment, the slurries (SAH) were cooled to room temperature, and samples were taken to measure the sugar concentration and inhibitor levels in the hydrolysate.
Before enzymatic hydrolysis, the pH of the SAH was adjusted to 5 by adding 10 M NaOH. Cellic CTec2 (kindly donated by Novozyme) was dosed to give a filter paper activity of 15 FPU/g biomass. Hydrolysis was performed in an orbital incubator (InforsMulitron, Infors Switzerland) at 50 °C, 150 rpm for 72 h. Samples were periodically taken for sugar analysis. The enzymatic hydrolysates of dilute sulfuric acid pretreated phragmites (SAEH) was transferred to 50 ml falcon tubes and centrifuged at 3,500 rpm (ST40R, Thermo Scientific) to remove sediments. The supernatant was stored in pre-sterilised flasks at 4 °C for detoxification and fermentation studies.
Gao K, & Rehmann L. (2016). Combined Detoxification and In-situ Product Removal by a Single Resin During Lignocellulosic Butanol Production. Scientific Reports, 6, 30533.
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3

DPPH Scavenging Activity of Marinated Chicken Wings

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DPPH free radical scavenging activity of the marinated chicken wings was measured
using methods described by Choe et al.
(2020) with slight modifications. Chicken wing samples (1 g) were
mixed with 80% methanol and homogenized Mixtures were then kept in a
shaker for 30 min at room temperature and centrifuged (ST 40R, Thermo Fisher
Scientific) at 1,409×g for 10 min at 4°C. The same analytic
procedure was followed as described in antioxidant activity of fruit juice
section.
Rupasinghe R.A., Alahakoon A.U., Alakolanga A.W., Jayasena D.D, & Jo C. (2022). Oxidative Stability of Vacuum-Packed Chicken Wings Marinated with Fruit Juices during Frozen Storage. Food Science of Animal Resources, 42(1), 61-72.
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4

Extraction and Analysis of Pyochelin Compounds

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Ethyl acetate (EtOAc; J.T. Baker and Fisher Scientific) and Optima-grade methanol (MeOH; Fisher Scientific) were used. P. aeruginosa PAO1 wild type or ΔpqsA cells were grown overnight in LB (RPI) at 37°C with 200 rpm shaking. Bacterial cells were centrifuged at 5,000 rpm (Biofuge pico, Heraeus) and washed with 0.5% chelex treated CAA. New cultures were seeded in 0.5% chelex treated CAA at a starting OD600 of 0.05 and incubated at 37°C with 200 rpm shaking. After 48 h the cultures were centrifuged at 3,800 rpm (Eppendorf Centrifuge 5810 R) for 30 min at room temperature. The supernatant was decanted and filtered using a Nalgene Rapid-flow Filter Unit (0.2 µm aPES membrane; Thermo Scientific). The cell free supernatant was acidified to pH 1.8–2 with 6 M HCl. Pyochelin and pyochelin methyl ester were extracted with 3 volumes EtOAc and evaporated to dryness in a Rotavapor (Büchi; Rotavapor R-300) or a SpeedVac vacuum concentrator (SPD131DDA SpeedVac Concentrator; Thermo Scientific). Dried samples were resuspended in MeOH and stored at – 20° C. Samples were centrifuged for 5 min at 10,000 rpm (Thermo; Sorvall ST 40R) to remove nonsoluble particulates and diluted as needed in MeOH containing 1 µM glycocholic acid.
Yokomaku D., Yamaguchi N, & Nasu M. (2000). Improved Direct Viable Count Procedure for Quantitative Estimation of Bacterial Viability in Freshwater Environments. Applied and Environmental Microbiology, 66(12), 5544-5548.
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5

Microbial Oil Extraction and Purification

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Example 19

A cell broth (1000 mL) containing microbial cells (Mortierella) was pasteurized at 70° C. for 1 hour. A demulsified lysed cell composition was formed by adding 15%, by weight broth, phosphoric acid (85% solution) to adjust the pH to 0.7 to 1.5 and heating the composition to 90° C. while agitating at a speed of 200 rpm for 50 hours. The microbial oil was separated from the demulsified lysed cell composition by adding 50% w/w solution of NaOH to adjust the pH 8.0-8.2 and centrifuging (Thermo Sorvall ST 40R) the composition at 8,000 g for 5 minutes to provide a crude oil, which yielded 42.61% ARA (by ARA weight). The crude oil had an AV of 16.1.

US10342772B2. Processes for obtaining microbial oil from microbial cells (2019-07-09). DSM IP ASSETS B.V. [NL]. Inventors: Mark Barker [US], Nasrin Tabayehnejad [US], Ginger Shank [US], Neil Leininger [US], Kirt Lyvell Matthews, Sr. [US].
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6

Microbial Oil Extraction from Crypthecodinium cohnii

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Example 23

A washed cell broth (750 g) containing microbial cells (Crypthecodinium cohnii) was pasteurized at 60° C. for 1 hour. The cells were mechanically lysed by passing the cell broth through a homogenizer (Microfluidizer M110) twice at 12,000 PSI. The lysed cell composition was placed in a round flask and demulsified by adding a 4%, by weight composition, sulfuric acid (98% pure) solution, heating to 90° C. while agitating at 200 RPM, and holding for 28 hours. The microbial oil was separated from the demulsified lysed cell composition by neutralizing the composition and adjusting the pH to 8.0 by addition of a 50% NaOH solution, and then centrifuging (Thermo Sorvall ST 40R) the composition at 8000 g for 5 minutes to provide a crude oil, which yielded 45% DHA (by DHA weight)(based on FAME analysis) and a crude oil with an AV of 12.35 and a PV of 1.09 meq.

US10342772B2. Processes for obtaining microbial oil from microbial cells (2019-07-09). DSM IP ASSETS B.V. [NL]. Inventors: Mark Barker [US], Nasrin Tabayehnejad [US], Ginger Shank [US], Neil Leininger [US], Kirt Lyvell Matthews, Sr. [US].
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7

Antioxidant Activity of Fruit Juices

Cited 1 time
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Fruit juices were analyzed for antioxidant activity using DPPH free radical
scavenging assay according to the method described by Choe et al. (2020) (link) with slight modifications. Methanolic
DPPH stock solution (0.1 mM) was prepared by dissolving 10 mg of DPPH powder in
125 mL of methanol. After that, 5 mL of fruit juice was mixed with 80%
methanol and kept in a shaker for 30 min at room temperature. The mixture was
then centrifuged (ST 40R, Thermo Fisher Scientific, Osterode, Germany) at
1,409×g for 10 min at 4°C and 200 μL of the supernatant was
mixed with 1 mL of DPPH solution. The mixture was shaken well and kept to stand
in a dark place for 30 min at room temperature. The absorbance of mixtures was
read at 517 nm using a spectrophotometer (UV-2005, J.P. Selecta, Barcelona,
Spain). The readings were compared with the control prepared with 200 μL
of 80% methanol and 1 mL of DPPH. The scavenging activity was calculated
using the following equation.
Rupasinghe R.A., Alahakoon A.U., Alakolanga A.W., Jayasena D.D, & Jo C. (2022). Oxidative Stability of Vacuum-Packed Chicken Wings Marinated with Fruit Juices during Frozen Storage. Food Science of Animal Resources, 42(1), 61-72.
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8

Extraction of DHA-rich Microbial Oil

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Example 3

An unwashed cell broth (550 kg) containing microbial cells (Crypthecodinium cohnii) was pasteurized at 60° C. for 1 hour. The unwashed cells were lysed by heating the cell broth to 90° C. and passing the broth through a mechanical homogenizer (Microfluidizer M210) twice at 12,000 PSI. The lysed cell composition was place in a tank and demulsified by: adding 1.85% caustic, by weight composition, of a 50% NaOH solution, heating the composition to 90° C., holding until a pH of 6.2 was achieved, and then adding 0.5%, by weight composition, Brewzyme LP (available from Dyadic International, Inc, Jupiter, Fla.) and allowing to react for 18 hours. The microbial oil was separated from the demulsified lysed cell composition by heating the composition to 75° C. and centrifuging (Thermo Sorvall ST 40R) the composition at 8000 g for 5 minutes to provide a crude oil, which yielded 80% DHA (by DHA weight) (based on crude oil weight analysis) and a crude oil with an AV of <0.5 and a PV of 0.14 meq.

US10364207B2. Processes for obtaining microbial oil from microbial cells (2019-07-30). DSM IP ASSETS B.V. [NL]. Inventors: Mark Barker [US], Nasrin Tabayehnejad [US], Ginger Shank [US], Neil Francis Leininger [US], Kirt Lyvell Matthews, Sr. [US].
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9

Microbial Oil Extraction from Schizochytrium

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Example 26

An unwashed cell broth (750 g) containing microbial cells (Schizochytrium sp.) was pasteurized at 60° C. for 1 hour and placed in a 1 L flask agitating at 250 RPM. The temperature was raised to 60° C. to decrease mix time due to viscosity and the pH raised to 7.5. A demulsified lysed cell composition was formed by: adding 3.5%%, by weight broth, of a 50% NaOH solution to adjust the pH to 12.28; heating to 90° C.; and allowing to react for 32 hours until the pH dropped to 7.88. The microbial oil was separated from the demulsified lysed cell composition by centrifuging (Thermo Sorvall ST 40R) the composition at 8000 g for 5 minutes to provide a crude oil, which yielded 88% DHA (by DHA weight) (based on FAME Analysis).

US10342772B2. Processes for obtaining microbial oil from microbial cells (2019-07-09). DSM IP ASSETS B.V. [NL]. Inventors: Mark Barker [US], Nasrin Tabayehnejad [US], Ginger Shank [US], Neil Leininger [US], Kirt Lyvell Matthews, Sr. [US].
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10

Fecal Metabolite Extraction Protocol

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Collected faecal material (combined sample from sub-container 1 and sub-container 2) was centrifuged (2279 ×
g, 40 min; Sorvall, ST 40 R, ThermoScientific, USA) and the pellet was extracted with 5 mL 80% methanol, vortexed (2500 rpm, 2 min; DVX-2500 Multi-Tube Vortexer), centrifuged (12,470 × g, 20 min) and the supernatant was kept at −80 °C until UPLC-MS/MS analysis (Fig. S4).
Camacho-Muñoz D., Waack J., Turner A.D., Lewis A.M., Lawton L.A, & Edwards C. (2021). Rapid uptake and slow depuration: Health risks following cyanotoxin accumulation in mussels?. Environmental Pollution (Barking, Essex : 1987), 271, 116400.
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