O dianisidin

Uric acid, hexadecyltrimethylammonium bromide, epinephrine, O-dianisidin, and sodium azide were purchased from Sigma-Aldrich Chemical Co. (Taufkirchen, Germany). Sodium hydroxide, hydrochloric acid, bovine serum albumin, sodium chloride, and Dimethylsufoxide (DMSO) were purchased from Carl-Roth (Kalshur, Germany). Nitric acid was purchased from Qualikems. Allopurinol was obtained from Aspen-Pharma (Ireland). Ether was purchased from a local pharmacy (Dschang, Cameroon). Griess reagent, Biuret reagent, and carbonate buffer were prepared in the laboratory with ingredients from Sigma-Aldrich Chemical Co. (Taufkirchen, Germany). ThiobarbitUric acid was purchased from Shanghai Zhanyun Chemical Co. Ltd (China). Trichloroacetic acid was purchased from Guangdon Guanghua Sci-Tech Co. Ltd (China).

Collagen Type II was prepared as previously described from bovine cartilage [35] . Complete Freund Adjuvant (CFA), leflunomide, hexadecyl trimethylammonium bromide (HTAB), and o-dianisidin were purchased from Sigma-Aldrich Chemical Company (St. Louis, MO, USA). Nimesulide was gifted from Alkan Pharmaceutical Co., (6^th of October city, Egypt).