NF-κB inhibitor (BAY11-7082), protein kinase C (PKC) inhibitor (GF-109203X), MEK inhibitor (PD98059), p38 inhibitor (SB203580), JNK inhibitor (SP600125), and phosphatidylinositol 3-kinase (PI3K) inhibitor (LY294002) were purchased from Cell Signaling Technology, Inc. LPS was obtained from Sigma-Aldrich. Enzyme-linked immunosorbent assay (Serti et al., 2010 (link)) kit for porcine IL-8 was purchased from R&D Systems. All inhibitors were reconstituted in dimethyl sulfoxide (DMSO), and DMSO was used as the solvent control for all experiments involving treatment with inhibitors. A Dual-Glo luciferase assay system was purchased from Promega. Antibodies against c-Jun, p-c-Jun, JNK, p-JNK, TAK-1, p-TAK-1, IκBα and p-IκBα were from Cell Signaling Technology, Inc. Antibody against β-actin was purchased from Sigma.
Gf 109203x
Manufactured by Cell Signaling Technology
GF-109203X is a specific and potent inhibitor of protein kinase C (PKC). It is a useful tool for studying the role of PKC in various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Bay 11 7082, by Cell Signaling Technology (2 mentions)
Pd98059, by Cell Signaling Technology (2 mentions)
Sb203580, by Cell Signaling Technology (2 mentions)
Ly294002, by Cell Signaling Technology (1 mentions)
Antibody against β actin, by Merck Group (1 mentions)
P iκbα, by Cell Signaling Technology (1 mentions)
P tak1, by Cell Signaling Technology (1 mentions)
Jnk inhibitor sp600125, by Cell Signaling Technology (1 mentions)
Enzyme linked immunosorbent assay, by R&D Systems (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Dual glo luciferase assay system, by Promega (1 mentions)
Sp600125, by Cell Signaling Technology (1 mentions)
Wortmannin, by Cell Signaling Technology (1 mentions)
2 protocols using gf 109203x
1
Signaling Pathway Inhibitors in Cell Assays
2
Studying NF-κB Activation Mechanisms
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To study the action mechanism, various pharmacological inhibitors were tested on PMA-mediated NF-κB activation. Pharmacological inhibitors were used such as Wortmannin, Bay 11-7082, Genistein, GF109203X, PD98059, SB203580, SP600125, and U-73122 (Cell Signaling Technology, Danvers, MA) for the inhibition of phosphoinositide 3-kinase (PI3K), IkB-α phosphorylation, protein tyrosine kinase (PTK), protein kinase C (PKC), MEK1, SAPK2 (p38), jun N-terminal kinase (JNK), and phospholipase C (PLC), respectively.
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