The following antibodies were used for mouse tissues immunohistochemistry and immunofluorescence: Mouse anti-Human TDP-43 (Novus 2E2–D3); Rabbit anti-Mouse TDP-43 N-Term (Proteintech 10782–2); Rabbit anti-Mouse TDP-43 C-Term (Proteintech 12892–1);Rabbit anti-Synaptophysin (Life Technologies Z66, 1:500); Mouse anti-Pan-Axonal neruofilament (Covance, SMI-312R, 1:1000). The following antibodies were used for cortex and spinal cord immunoblot: Mouse anti-Human TDP-43 (Novus 2E2–D3); Rabbit anti-Mouse TDP-43 N-Term (Proteintech 10782–2); Rabbit anti-Mouse TDP-43 C-Term (Proteintech 12892–1); Mouse anti-GAPDH (Sigma, GAPDH-71.1).The following antibodies were used for i3Neuron stable cell lines immunoblot: Rabbit anti-TDP-43 (Proteintech 10782–2-AP 1:1000); Mouse anti-FLAG (Sigma F1804, 1:5000); Rabbit anti-GAPDH (CST 2118, 1:1000); Mouse anti-Tubulin (Thermofisher 14–4502–82, 1:1000). The following antibodies were used for QBI-293 stable cell lines immunoblot: Mouse anti-TDP-43 (abcam ab104223, 1:2000); Rabbit anti-GFP (Cell signalling mAb 2956, 1:1000); Mouse anti-Beta Actin (Proteintech 66009–1, 1:2000). The following antibody was used for HEK-293 cells immunoblot: Rabbit anti-Mouse TDP-43 N-Term (Proteintech 10782–2, 1:2000).
Carmen-Orozco R.P., Tsao W., Ye Y., Sinha I.R., Chang K., Trinh V., Chung W., Bowden K., Troncoso J.C., Blackshaw S., Hayes L.R., Sun S., Wong P.C, & Ling J.P. (2023). Elevated nuclear TDP-43 induces constitutive exon skipping. bioRxiv.
+ Open protocol