Isolated chromaffin cells were plated onto high precision coverslips (Zeiss), fixed 3–5 days later and immunostained as described above. Samples were imaged on a Nikon Ti microscope equipped with a 100× 1.49 NA Apo TIRF objective and an Andor Xyla sCMOS camera. Structured illumination images were acquired as a 15-slice z-stack with 120 nm step size and reconstructed using Nikon Elements software outfitted with the NIS-A N-SIM analysis module. Colocalization was quantified with the Coloc2 module in ImageJ.
High precision coverslips
Manufactured by Zeiss
High precision coverslips are thin, flat glass or plastic pieces used to cover and protect specimen samples in microscopy. They are designed to provide a uniform, optically-flat surface to enhance image quality and clarity during microscopic analysis.
Automatically generated - may contain errors
2 protocols using high precision coverslips
1
Chromaffin Cell Imaging Protocol
2
Chromaffin Cell Imaging Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Isolated chromaffin cells were plated onto high precision coverslips (Zeiss), fixed 3–5 days later and immunostained as described above. Samples were imaged on a Nikon Ti microscope equipped with a 100× 1.49 NA Apo TIRF objective and an Andor Xyla sCMOS camera. Structured illumination images were acquired as a 15-slice z-stack with 120 nm step size and reconstructed using Nikon Elements software outfitted with the NIS-A N-SIM analysis module. Colocalization was quantified with the Coloc2 module in ImageJ.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!