Water (HPLC grade), acetonitrile (HPLC grade), 1 M NaBH3CN (in THF) and human immunoglobulin G (IgG) were from Sigma Aldrich (St. Louis, MO, USA). Enbrel (etanercept) was kindly provided by the Semmelweis University, Budapest, Hungary. Peptide N-glycosidase F (PNGase F) enzyme (200 mU) and the specific exoglycosidases of Sialidase A (α(2 → 3,6,8,9)R; ), β-galactosidase (Jack Bean; β(1 → 3,4,6)R; ) and β-N-Acetyl-hexosaminidase (Jack Bean; β(1 → 2,3,4,6)R; ) were from ProZyme (Hayward, CA, USA).
Human immunoglobulin g igg
Manufactured by Merck Group
Sourced in United States
Human immunoglobulin G (IgG) is a type of antibody protein found in the blood. IgG is the most common antibody in the human body and plays a crucial role in the immune system's response to infections and diseases.
Automatically generated - may contain errors
Lab products found in correlation
Lsrfortessa, by BD (1 mentions)
Lymphocyte separation medium, by Corning (1 mentions)
Anti humancd3 clone ucht1, by BioLegend (1 mentions)
Cd8 clone rpa t8, by BioLegend (1 mentions)
Cx3cr1 clone 2a9 1, by BioLegend (1 mentions)
Human transferrin, by Merck Group (1 mentions)
Potassium chloride (kcl), by Merck Group (1 mentions)
Recombinant human serum albumin, by Merck Group (1 mentions)
Hen egg lysozyme, by Merck Group (1 mentions)
Melon gel igg spin purification kit, by Thermo Fisher Scientific (1 mentions)
7 protocols using human immunoglobulin g igg
1
Glycopeptide Analysis of Therapeutic Antibodies
2
Isolation and Analysis of Human CX3CR1+ T Cells
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Using lymphocyte separation medium (Corning) density gradient centrifugation, we isolated peripheral blood mononuclear cells (PBMCs). Staining of CX3C chemokine receptor 1 (CX3CR1) in T cells was performed as described before [23 (link), 24 (link)]. In brief, fresh or cryopreserved PBMCs were incubated with Fc block with human immunoglobulin G (IgG) (Sigma) at 12 mg/mL for 20 min. Anti-human CD3 (clone UCHT1), CD8 (clone RPA-T8), and CX3CR1 (clone 2A9-1) antibodies were obtained from BioLegend, and anti-CD4 (clone RPA-T4) antibody was from BD Biosciences for flow cytometry. LSRFortessa (BD) was used for sample acquisition, and FlowJo software v10.1.5 (FlowJo LLC) was used for sample analysis.
3
Synthesis and Characterization of Nanoparticles
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All
chemicals used in the nanoparticle synthesis
were purchased from Merck and used as received unless otherwise indicated.
The exceptions were (1-cyano-2-ethoxy-2-oxoethylidenaminooxy)dimethylamino-morpholino-carbenium
hexafluorophosphate (COMU) that was purchased from Carl Roth, 2-ethyl-2-oxazoline
that was dried over CaH2 before use, and methyl-p-toluenesulfonate that was distilled before use. (4-(2-Hydroxyethyl)-1-piperazineethanesulfonic
acid) (HEPES), NaCl, KCl, recombinant human serum albumin, hen egg
lysozyme, human transferrin, and human immunoglobulin G (IgG) from
serum were purchased from Sigma-Aldrich. Regenerated cellulose 0.22
μm syringe filters were purchased from Bruckner Analysentechnik.
chemicals used in the nanoparticle synthesis
were purchased from Merck and used as received unless otherwise indicated.
The exceptions were (1-cyano-2-ethoxy-2-oxoethylidenaminooxy)dimethylamino-morpholino-carbenium
hexafluorophosphate (COMU) that was purchased from Carl Roth, 2-ethyl-2-oxazoline
that was dried over CaH2 before use, and methyl-p-toluenesulfonate that was distilled before use. (4-(2-Hydroxyethyl)-1-piperazineethanesulfonic
acid) (HEPES), NaCl, KCl, recombinant human serum albumin, hen egg
lysozyme, human transferrin, and human immunoglobulin G (IgG) from
serum were purchased from Sigma-Aldrich. Regenerated cellulose 0.22
μm syringe filters were purchased from Bruckner Analysentechnik.
4
Quantifying HSV-1 Infectivity in DRG Explants
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A group of three DRG explants per well in triplicate experiment was cultured for 3 days prior to be infected with replication-competent syn mutant HSV-1(KOS-804) (Little and Schaffer 1981 (link)) or mock infected with PBS alone as a control for 2 h at 37 °C (Fig. 2S ). After removal of the unbound virus, DRG explants were overlaid with SFM and incubated at 37 °C until the time of harvest of inoculums (12, 24, and 36 h). Inoculum of DRG explants infected with HSV-1 or mock treated was laid on Vero cells cultured in triplicate to visualize plaque formation. In order to block secondary plaque formation, human immunoglobulin G (IgG; 1:10 Sigma-Aldrich, St. Louis, MO) was added to the inoculums. The Vero cells were washed with PBS buffer, fixed in ethanol 80%, and stained with Giemsa stain. Infectivity was quantified by counting the number of plaques.
5
Cell Line Characterization and Culture Conditions
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The SQ20B cell line was a gift from Dr. Anjali Gupta (The University of Iowa). The SCCVII cell line was a gift from Dr. George Weiner. The TUBO-human EGFR (TUBO-hEGFR) cell line was gifted to our lab from Dr. Yang-Xin Fu (Department of Pathology, University of Chicago, IL)20 (link), and the mEERL cell line was a gift from Dr. Paola Vermeer (Department of Surgery, University of South Dakota Sanford School of Medicine, SD)21 (link). All cell lines were authenticated by short tandem repeat profiling and used over a course of no more than 3 months after resuscitation of frozen aliquots. All cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) containing 10% fetal bovine serum (FBS) and 0.1% gentamicin, except for mEERL which was cultured in DMEM supplemented with 40.5% 1:1 DMEM/Hams F12, 10% FBS, 0.1% gentamicin, 0.005% hydrocortisone, 0.05% transferrin, 0.05% insulin, 0.0014% tri-iodo-thyronine and 0.005% EGF. Cells were cultured in a humidified incubator at 37 °C and 5% CO2. Motolimod (VTX-2337) was purchased from AdooQ Bioscience (Irvine, CA). Cetuximab was obtained from the inpatient pharmacy at the University of Iowa Hospitals and Clinics. Human immunoglobulin G (IgG) was purchased from Sigma-Aldrich (St. Louis, MO).
6
Monoclonal Antibodies for Platelet Integrin Detection
7
Preparation of Moss Samples for Analysis
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Human immunoglobulin G (IgG) was purchased from Sigma (St. Louis, MO). Ethylene glycol was supplied by Fluka (Switzerland). All other chemicals were of analytical grade and used without any purification or separation process. Deionized water was produced by using Millipore, Simplicity V R (18.2 MXcm) and was used for the preparation of all solutions.
Preparation of S. papillosissima samples S. papillosissima samples were collected from natural habitat which they grow at Aydın, Pas ¸a yaylası vicinity. Plant materials were cleaned by cutting the dead parts and washing the sample in order remove dust and debris by using distilled water. Cleaned moss samples were dried at 40 C for 1 week and then grinded in order to separate the samples for particle size.
Preparation of S. papillosissima samples S. papillosissima samples were collected from natural habitat which they grow at Aydın, Pas ¸a yaylası vicinity. Plant materials were cleaned by cutting the dead parts and washing the sample in order remove dust and debris by using distilled water. Cleaned moss samples were dried at 40 C for 1 week and then grinded in order to separate the samples for particle size.
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