Human breast cancer cell lines MCF-7 (HTB-22, ATCC), T-47D (HTB-133, ATCC), ZR-75-1 (CRL-1500, ATCC) and BT-474 (HTB-20, ATCC) were obtained from the American Type Culture Collection. The cells were grown in DMEM with L-Glutamine (MCF-7 and BT-474, PAN Biotech, Aidenbach, Germany) or RPMI-1640 with L-Glutamine (ZR-75-1 and T-47D, PAN Biotech) supplemented with 10 % FCS (Gibco, Life Technologies, Carlsbad, CA) and 1 % penicillin/streptomycin (Gibco). Culture media for T-47D, MCF-7 and BT-474 additionally contained 0,1 % bovine insulin (Sigma. St. Louis, MO). The tamoxifen-resistant cell lines (MCF-7 Tam1, T-47D Tam1 & Tam2, ZR-75-1 Tam1 & Tam2, BT-474 Tam1 & Tam2) were derived from the parental cell lines by continuous exposure to 4-OH-tamoxifen (Sigma, 1 μM in ethanol) for 8–12 months. Culture media was replaced every 2–3 days. All cells were incubated at 37 °C with 5 % CO2 and passaged when ca 80 % confluent. The approximate doubling times of the cells were as follows: parental MCF-7, T-47D, ZR-75-1 and BT-474: 1–3 days. Resistant MCF-7 Tam1, T-47D Tam1 and Tam2: 1–2 weeks, ZR-75-1 Tam1 and Tam2: > 1 week, BT-474 Tam1 and Tam2: 2 weeks. The cells were free of mycoplasma and verified for their authenticity (Technology Centre, Institute for Molecular Medicine Finland, Helsinki, Finland).
Bt474
Manufactured by Merck Group
Sourced in United States, Germany
The BT474 is a laboratory equipment product manufactured by Merck Group. It is designed for performing various scientific experiments and analyses. The core function of the BT474 is to provide a controlled and reliable environment for conducting research and testing.
Automatically generated - may contain errors
Lab products found in correlation
Bt474, by American Type Culture Collection (21 mentions)
Mcf 7, by American Type Culture Collection (16 mentions)
Mcf 7, by Merck Group (15 mentions)
Sk br 3, by Merck Group (15 mentions)
Skbr3, by American Type Culture Collection (12 mentions)
Mda mb 231, by American Type Culture Collection (11 mentions)
Mda mb 231, by Merck Group (11 mentions)
Fetal bovine serum (fbs), by Merck Group (8 mentions)
Rpmi 1640 medium, by Merck Group (7 mentions)
Rpmi 1640, by Merck Group (6 mentions)
L glutamine, by Thermo Fisher Scientific (5 mentions)
Mda mb 468, by Merck Group (5 mentions)
Zr 75 1, by Merck Group (4 mentions)
Dmem medium, by Merck Group (4 mentions)
Mda mb 468, by American Type Culture Collection (4 mentions)
Skov3, by American Type Culture Collection (3 mentions)
Skov3, by Merck Group (3 mentions)
Zr 75 1, by American Type Culture Collection (3 mentions)
Jimt 1, by Merck Group (3 mentions)
Penicillin streptomycin, by Merck Group (3 mentions)
29 protocols using bt474
1
Breast Cancer Cell Lines and Tamoxifen Resistance
2
Epigenetic Regulation of Prostate and Breast Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LNCaP, PC3, and DU145 human prostate cancer cells and the human breast cancer cells MDA-MB-231, and BT-474 were obtained from the American Type Culture Collection (Manassaa, VA, USA) and routinely cultured in the appropriate medium. Cells were maintained at 37 °C in a humidified atmosphere of 95% air and 5% CO2.
For the treatment with the demethylating agent 5’-aza-2’ deoxycytidine (Sigma-Aldrich, St Louis, MO, USA), LNCaP, PC3, DU145, MDA-MB-231 and BT-474 cells were treated with 2 or 5 µM (Sigma-Aldrich, St Louis, MO, USA) for 3 days. Treatment was refreshed every 24 h.
Gfi1 methylation analysis were retrospectively evaluated in 44 breast tumor samples and 39 prostate tumor samples from paraffin-embedded blocks. Gfi1 expression was evaluated in 91 prostate tumor samples and 10 prostate normal tissues. Disease-free survival analysis was performed with same cohort in a previous study [17 (link)]. Clinical evolution based on PSA and imaging was recorded. The primary endpoint assessed was disease-specific survival. The study was approved by the Ethics Committee of Hospital Universitario de Getafe (A17-11 of 27 October 2011).
For the treatment with the demethylating agent 5’-aza-2’ deoxycytidine (Sigma-Aldrich, St Louis, MO, USA), LNCaP, PC3, DU145, MDA-MB-231 and BT-474 cells were treated with 2 or 5 µM (Sigma-Aldrich, St Louis, MO, USA) for 3 days. Treatment was refreshed every 24 h.
Gfi1 methylation analysis were retrospectively evaluated in 44 breast tumor samples and 39 prostate tumor samples from paraffin-embedded blocks. Gfi1 expression was evaluated in 91 prostate tumor samples and 10 prostate normal tissues. Disease-free survival analysis was performed with same cohort in a previous study [17 (link)]. Clinical evolution based on PSA and imaging was recorded. The primary endpoint assessed was disease-specific survival. The study was approved by the Ethics Committee of Hospital Universitario de Getafe (A17-11 of 27 October 2011).
3
Quantifying Radioactive Tracer Uptake in HER2-Expressing Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A Cyclone Storage Phosphor System and OptiQuant image analysis software (PerkinElmer, Waltham, MA, USA) were used for measuring the radioactivity distribution on instant thin-layer chromatography (iTLC) strips.
In vitro cell studies were performed using HER2-expressing ovarian cancer SKOV-3 and breast cancer BT-474 cells, both obtained from the American Type Culture Collection (ATCC, Manassas, MA, USA). Ramos lymphoma cells (from ATCC) were used to establish HER2-negative xenografts. Cells were cultured in RPMI1640 medium (Sigma-Aldrich, St. Louis, MO, USA), supplemented with 10% for SKOV-3 or 20% for BT-474, of foetal calf serum, 2 mM L-glutamine, 100 IU/mL penicillin, and 100 mg/mL streptomycin.
In vitro cell studies were performed using HER2-expressing ovarian cancer SKOV-3 and breast cancer BT-474 cells, both obtained from the American Type Culture Collection (ATCC, Manassas, MA, USA). Ramos lymphoma cells (from ATCC) were used to establish HER2-negative xenografts. Cells were cultured in RPMI1640 medium (Sigma-Aldrich, St. Louis, MO, USA), supplemented with 10% for SKOV-3 or 20% for BT-474, of foetal calf serum, 2 mM L-glutamine, 100 IU/mL penicillin, and 100 mg/mL streptomycin.
4
Culturing Breast Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human breast cancer cell lines MCF7, T47D, ZR-75-1 (ER+/HER2−), BT474 (ER+/HER2+), SKBR3 and JIMT-1 (ER−/HER2+) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). The MCF7, T47D, ZR-75-1, SKBR3 and JIMT-1 cell lines were cultured in an RPMI 1640 medium (Sigma, St. Louis, MO, USA), while the BT474 cell line was cultured in a DMEM medium (Sigma). All culture media were supplemented with 10% fetal bovine serum (FBS) (Sigma), an antibiotic-antimycotic solution (1×) (Sigma), and l -glutamine (2 mM) (Invitrogen GmbH, Karslruhe, Germany). The cultures were maintained in an incubator at 37 °C under 5% CO2.
5
Cell Line Culture Conditions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SKOV3, BT474, SKBR3, AU565, MCF7, and A549 cell lines were obtained from American Type Culture Collection (ATCC) and cultured in McCoy’s 5A medium (SKOV3, SKBR3), RPMI-1640 medium (AU565, BT474), or Dulbecco’s modified Eagle medium (MCF7, A549) supplemented with 10% FBS (20% for BT474 cells) (Sigma-Aldrich), 2 mM L-glutamine, 100 IU/mL penicillin, and 100 µg/mL streptomycin in a humidified incubator at 37 °C in 5% CO2 atmosphere.
6
Characterization of Breast Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell lines were obtained from ATCC (HCC2218, HCC1954, HCC1419, ZR-75-30, AU565, NCI-2170, BT-474, SK-BR-3, NCI-N87, SKOV3, MCF7), Sigma (OE-19), DSMZ (JIMT-1), Cedarlane (ZR-75-1) and AddexBio (MDA-MB-175-VII, MDA-MB-468). Cell lines were authenticated by supplier and were used from source and expanded for a maximum of 20 passages. Cell lines were routinely spot check tested for mycoplasma, all tests were negative. Cell line supplier and catalog numbers are provided in Supplementary Table 14 . Trastuzumab (Herceptin®) and pertuzumab (Perjeta®) were purchased from Crown Bio and Xentech, respectively. Zanidatamab was prepared according to standard manufacturing procedures (CMC Bio). A non-specific IgG1 Ab that binds to respiratory syncytial virus (RSV) protein F, palivizumab, was used as a negative control (neg. control or NC) and was produced under standard expression and purification conditions. Human complement serum, baby rabbit complement serum and pooled murine complement serum were obtained from Cedarlane. Mouse strain serum (male and female Balb/c, male and female CB17 SCID) was collected under protocols that were approved by the Animal Care Committee at University of British Columbia.
7
Culturing Breast Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human breast cancer cell lines MCF-7, BT474, MDA-MB-231 and SUM 1315 were obtained from American Type Culture Collection (ATCC, VA, USA). The cells were cultured in a humidified atmosphere of 5% CO2 at 37°C and fed with complete high glucose Dulbecco's modified Eagle medium (DMEM), supplemented with 10% fetal bovine serum, 1% penicillin–streptomycin solution. For 17β-estradiol treatment, MCF-7 and BT474 were cultured in DMEM without phenol red, supplemented with 5% steroid-depleted foetal bovine serum (BI, Israel) for 4 days prior to 17β-estradiol (estrogen, E2, Sigma, USA) treatment.
8
Breast Cancer Cell Line Cultivation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The BC cell lines were purchased from ATCC (VA, USA) (Additional file 1 : Table S1). MCF7, T47D, BT-549 and MDA-MB-231 were cultured in RPMI (Sigma Aldrich, USA), while BT-474, SkBr3, MDA-MB-468, BT-20 and Hs578T were cultured in DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Sigma Aldrich, USA). The immortalized human mammary epithelial cells hTERT-HME1 (ATCC, USA) were grown in DMEM/F-12 mixture medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Sigma Aldrich, USA). The human mammary epithelial cells HMEpC were purchased from cell applications (CA, USA) and maintained in defined mammary epithelial cell medium provided by the company (Cell applications, USA).
9
Culturing Human Glioblastoma and Breast Cancer Cells
10
Breast Cancer Cell Line Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Breast cancer cell lines of different subtypes utilized for this study are as follows: (i) MCF7 (luminal A, ER+PR+HER2−), (ii) MDA-MB-231 (TNBC, ER−PR−HER2−) were obtained from the American Type Culture Collection (ATCC), Manassas, VA, USA; while (iii) MDA-MB-453 (AR+ER−PR−HER2+FGF+), (iv) BT474 (HER2-enriched, ER−PR−HER2+), and (v) SKBR3 (HER2-enriched) were obtained from M.G.N., SJRI, India [53 (link)]. The phenotypic characterization of cell lines was performed as reported previously [31 (link)]. The MCF7, BT474, MDA-MB-453, and SKBR3 cells were maintained in the Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA) containing 25 mM of HEPES and 3.6 g/L of sodium bicarbonate supplemented with 10% fetal bovine serum (FBS, Sigma-Aldrich) and 1% antibiotic antimycotic cocktail (Invitrogen, Carlsbad, CA, USA). The MDA-MB-231 cells were cultured in an L-15 medium (Sigma-Aldrich). All cells were maintained in humidified incubators at 37 °C with 5% CO2, and functional cells up to the 10th passage were used for all experiments in this study.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!