Sc 268

Manufactured by Abcam

Sc-268 is a research-use-only product designed for use in various laboratory applications. It is a monoclonal antibody that can be used to detect the target antigen in samples. Further details on the intended use or performance of this product are not available.

Automatically generated - may contain errors

Lab products found in correlation

Ab60721, by Abcam (1 mentions) Ab23345, by Santa Cruz Biotechnology (1 mentions) Sc 5279, by Santa Cruz Biotechnology (1 mentions) Sc 17320, by Santa Cruz Biotechnology (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Roche (1 mentions) Ab39398, by Abcam (1 mentions) Rm9102s0, by Abcam (1 mentions)

2 protocols using sc 268

Immunofluorescence Staining of Stem Cells

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Cells were fixed with 4% paraformaldehyde for 15 min and washed by PBS three times. Then, they were permeabilized with PBS containing 0.1% Triton X-100 and 3% donkey serum for 1 h. Cells were incubated in primary antibody overnight at 4 °C and secondary antibody at room temperature for 1 h. Cells were washed by PBS with 0.1% Tween-20 three times after every step. The nuclei were stained with DAPI (Roche Life Science, 10236276001). The antibodies were anti-FOXA2 (1:200; ab60721; Abcam), anti-b-III TUBULIN (1:300; Santa Cruz, sc-80016), anti-NANOG (1:100; Abcam, ab80892), anti-CDX2 (CDX2-88; Biogenex, AM392), anti-GATA3 (1:200; Santa Cruz, sc-268), anti-EOMES (1:200; Abcam, AB23345), and anti-OCT4 (1:200; Santa Cruz, sc-5279), anti-SOX2 (1:200; Santa Cruz, sc-17320).

Li H., Zhao C., Xu J., Xu Y., Cheng C., Liu Y., Wang T., Du Y., Xie L., Zhao J., Han Y., Wang X., Bai Y, & Deng H. (2018). Rapid generation of gene-targeted EPS-derived mouse models through tetraploid complementation. Protein & Cell, 10(1), 20-30.

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Immunohistochemical Analysis of Breast Tumors

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Sections were deparaffinized and rehydrated prior to antigen unmasking according to manufacturer’s instructions (Vector Labs, #H-3300), washed once with phosphate buffer saline and blocked with 0.5% casein and 0.1% Tween20/PBS for 1 hr at room temperature. Primary antibodies to ER (C1355) (Millipore/Upstate, #06-935), PR (Fisher Scientific, RM-9102-S0), Foxa1 (Abcam, ab23738), Gata3 (Santa cruz, SC-268), or phospho-IGF1 receptor (IGFR; Abcam, Ab39398) were diluted in Superblock Blocking Buffer (Pierce, #37515) and incubation was done at 4 degrees C, overnight. The slides were then washed, followed by incubation with peroxidase-conjugated secondary antibody, washed and counterstained with hematoxylin. Histopathological characteristics of the tumors were reviewed by two observers blinded to the experimental details of the mouse models. Estrogen receptor status was scored using the Allred method(26 (link)).

Nguyen D.H., Ouyang H., Mao J.H., Hlatky L, & Barcellos-Hoff M.H. (2014). Distinct luminal type mammary carcinomas arise from orthotopic Trp53 null mammary transplantation of juvenile versus adult mice. Cancer research, 74(23), 7149-7158.

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