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H00056339 b01p

Manufactured by Merck Group
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The H00056339-B01P is a laboratory equipment product from Merck Group. It serves a core function in laboratory settings, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

Automatically generated - may contain errors

Lab products found in correlation

Abe572, by Merck Group (3 mentions) Spa 810, by PhosphoSolutions (2 mentions) Hpa038002, by Santa Cruz Biotechnology (2 mentions) Sc 374280, by Merck Group (2 mentions) Alexa fluor 488 donkey anti mouse secondary antibody, by Thermo Fisher Scientific (2 mentions) Alexa fluor 546 donkey anti rabbit secondary antibody, by Thermo Fisher Scientific (2 mentions) Ab99080, by Proteintech (1 mentions) Anti β actin, by Merck Group (1 mentions) 35s methionine, by PerkinElmer (1 mentions)

3 protocols using h00056339 b01p

1

HeLa and MEF Cells for m6A Research

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HeLa (cervical cancer) was originally purchased from ATCC and MEF cells were a gift from D.J. Kwiatkowski (Harvard Medical School). Cells were not authenticated recently but tested negative for mycoplasma contamination. Both cells were maintained in Dulbecco's Modified Eagle's Medium (DMEM) with 10% fetal bovine serum (FBS). Antibodies used in the experiments are listed below: anti-YTHDF2 (Proteintech 24744-1-AP, 1:1000 WB, 1:600 IF); anti-Hsp70 (Stressgen SPA-810, 1:1000 WB); anti-FTO (Phosphosolutions 597-Fto, 1:1000 WB, 1:600 IF); anti-METTL3 (Abnova H00056339-B01P, 1:1000 WB, 1:600 IF); anti-METTL14 (sigma HPA038002, 1:1000 WB, 1:600 IF); anti-WTAP (Santa Cruz sc-374280, 1:1000 WB, 1:600 IF); anti-m6A (Millipore ABE572, 1:1000 m6A immunoblotting); Alexa Fluor 488 donkey anti-mouse secondary antibody (Invitrogen A21202. 1:600 IF); Alexa Fluor 546 donkey anti-rabbit secondary antibody (Invitrogen A10040, 1:600 IF).
Zhou J., Wan J., Gao X., Zhang X, & Qian S.B. (2015). Dynamic m6A mRNA methylation directs translational control of heat shock response. Nature, 526(7574), 591-594.
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2

Immunoblotting analysis of m6A regulators

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293T and MEF cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) with 10% fetal bovine serum. Antibodies used in this study are listed as follows: anti-YTHDF1 (Abcam ab99080, 1:1,000 WB), anti-YTHDF2 (Proteintech 24744-1-AP, 1:1000 WB), anti-YTHDF3 (Santa Cruz sc-377119, 1:1000 WB), anti-YTHDC2 (Abcam ab176846, 1:1000 WB), anti-METTL3 (Abnova H00056339-B01P, 1:1000 WB), anti-METTL14 (Sigma HPA038002, 1:1000 WB), anti-FTO (Phosphosolutions 597-FTO, 1:1000 WB), anti-ALKBH5 ((Proteintech 16837-1-AP, 1:1000 WB), anti-puromycin (Developmental Studies Hybridoma Bank-PMY-2A4, 1:100 WB), anti-Flag (M2) antibody (F1804), anti-m6A (Millipore ABE572) and anti-β-actin (Sigma-A5441, 1:2,000 WB). [35S]-methionine was purchased from PerkinElmer (#NEG772007MC).
Mao Y., Dong L., Liu X.M., Guo J., Ma H., Shen B, & Qian S.B. (2019). m6A in mRNA coding regions promotes translation via the RNA helicase-containing YTHDC2. Nature Communications, 10, 5332.
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3

HeLa and MEF Cells for m6A Research

Check if the same lab product or an alternative is used in the 5 most similar protocols
HeLa (cervical cancer) was originally purchased from ATCC and MEF cells were a gift from D.J. Kwiatkowski (Harvard Medical School). Cells were not authenticated recently but tested negative for mycoplasma contamination. Both cells were maintained in Dulbecco's Modified Eagle's Medium (DMEM) with 10% fetal bovine serum (FBS). Antibodies used in the experiments are listed below: anti-YTHDF2 (Proteintech 24744-1-AP, 1:1000 WB, 1:600 IF); anti-Hsp70 (Stressgen SPA-810, 1:1000 WB); anti-FTO (Phosphosolutions 597-Fto, 1:1000 WB, 1:600 IF); anti-METTL3 (Abnova H00056339-B01P, 1:1000 WB, 1:600 IF); anti-METTL14 (sigma HPA038002, 1:1000 WB, 1:600 IF); anti-WTAP (Santa Cruz sc-374280, 1:1000 WB, 1:600 IF); anti-m6A (Millipore ABE572, 1:1000 m6A immunoblotting); Alexa Fluor 488 donkey anti-mouse secondary antibody (Invitrogen A21202. 1:600 IF); Alexa Fluor 546 donkey anti-rabbit secondary antibody (Invitrogen A10040, 1:600 IF).
Zhou J., Wan J., Gao X., Zhang X, & Qian S.B. (2015). Dynamic m6A mRNA methylation directs translational control of heat shock response. Nature, 526(7574), 591-594.
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