Anti fak 610088

Manufactured by BD

Sourced in United States

Anti-FAK (610088) is a laboratory reagent used in research applications. It functions as an antibody that specifically binds to the Focal Adhesion Kinase (FAK) protein. FAK is an important signaling molecule involved in various cellular processes. The Anti-FAK (610088) reagent can be utilized in research techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to study the expression, localization, and interactions of the FAK protein.

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Lab products found in correlation

Anti cortactin 05 180, by Merck Group (1 mentions) Anti tubulin t5168, by Merck Group (1 mentions) Anti p tyr 397 fak, by BD (1 mentions) Gapdh, by Merck Group (1 mentions) Ecl detection system, by GE Healthcare (1 mentions) Anti vinculin v9131, by Merck Group (1 mentions) Rhodamine labeled phalloidin, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit 800cw, by LI COR (1 mentions) Goat anti mouse 680lt, by LI COR (1 mentions) Anti β actin clone ac 15 a5441, by Merck Group (1 mentions) L histidinol dihydrochloride h6647, by Merck Group (1 mentions) Anti crkl sc 319, by Santa Cruz Biotechnology (1 mentions) Anti gfp 11814460001, by Roche (1 mentions) Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Anti-FAK (23 citations) Anti-FAK (clone 77; 610088) (3 citations)

3 protocols using anti fak 610088

Immunofluorescence and Western Blotting

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For immunofluorescence, anti-FAK (610088) was obtained from BD; anti-cortactin (05-180) from Millipore; anti-phospho-Y118 paxillin (44-722G), anti-paxillin (AHO0492) and anti-phospho-Y397 FAK (44-625G) from Invitrogen; phalloidin 568 (A12380), phalloidin 647 (A22287) and Alexa Fluor-conjugated secondary antibodies from Molecular Probes. For Western blotting and co-immunoprecipitation, anti-phospho-Y397 FAK (44-625G), anti-paxillin (AHO0492), was obtained from Invitrogen; eGFP (66002-1-IG) from Proteintech anti-FAK (610088) from BD; anti-b-actin (MA1115) from Boster; and HRP conjugated secondary antibodies from Biorad.

Mousson A., Legrand M., Steffan T., Vauchelles R., Carl P., Gies J.P., Lehmann M., Zuber G., De Mey J., Dujardin D., Sick E, & Rondé P. (2021). Inhibiting FAK–Paxillin Interaction Reduces Migration and Invadopodia-Mediated Matrix Degradation in Metastatic Melanoma Cells. Cancers, 13(8), 1871.

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Immunoprecipitation and Rac Activation Assay

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Lysates (at 2 mg/mL protein concentration) were prepared as described [13 (link)]. The rabbit polyclonal anti-FlnA antibody (sc-28284; Santa Cruz Biotechnology) was used for immunoprecipitating FlnA. AR was revealed using the mouse monoclonal anti-AR antibody (441, sc-7305, Santa Cruz). The rabbit polyclonal anti-FlnA antibody (4762S; Cell Signaling, Danvers, MA, USA) was used to detect FlnA in the immune-complexes and in cell lysates. The mouse monoclonal anti-FAK (610088, BD Transduction Laboratories), anti P-Tyr397 FAK (611722; BD Transduction Laboratories), anti-GAPDH (E-AB-20032; Elabscience, Houston, TX, USA) and anti-Tubulin (T5168; Sigma-Aldrich) antibodies were used for the detection of FAK, P-Tyr397 FAK, GAPDH and tubulin, respectively. Rac pull-down assay was done [8 (link)] using the Rac activation kit (Upstate Biotechnology, Burlington, MA, USA). Immune-reactive proteins were revealed by the ECL detection system (GE Healthcare, Chicago, IL, USA).

Di Donato M., Giovannelli P., Barone M.V., Auricchio F., Castoria G, & Migliaccio A. (2021). A Small Peptide Targeting the Ligand-Induced Androgen Receptor/Filamin a Interaction Inhibits the Invasive Phenotype of Prostate Cancer Cells. Cells, 11(1), 14.

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Fibronectin and Histidinol Immunoblotting Protocol

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Fibronectin from human plasma (F0895) and l-histidinol dihydrochloride (H6647) were obtained from Sigma-Aldrich. Hygromycin (#ant-hg) was obtained from InvivoGen. For Western blot, anti-Pyk2 (3292) was obtained from Cell Signaling Technology; anti-FAK (610088) and anti CrkI/II (610036) were obtained from BD Transduction Laboratories; anti-CrkL (sc-319) was obtained from Santa Cruz Biotechnology; anti-GFP (11814460001) was obtained from Roche Life Science; anti–β-actin (clone AC-15) (A5441) was obtained from Sigma-Aldrich; anti–HSC-70 (1427) was obtained from Abcam. Secondary antibodies (goat anti-mouse 680LT and goat anti-rabbit 800CW) were obtained from LI-COR Biosciences. For immunofluorescence, antivinculin (V9131) was obtained from Sigma-Aldrich; rhodamine-labeled phalloidin and Alexa Fluor–conjugated secondary antibodies were obtained from Molecular Probes.

Lukic N., Lapetina S., Grobe H., Srikanth K.D., Twafra S., Solomon J., Sneh T., Gendler M., Zaidel-Bar R, & Gil-Henn H. (2021). Pyk2 regulates cell-edge protrusion dynamics by interacting with Crk. Molecular Biology of the Cell, 32(21), ar17.

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