B6.129p2 il2tm1hor j il2

All mice were maintained and bred in-house in an American Association of Laboratory Animal Care-approved facility at the University of California, Berkeley. All procedures were approved by the University of California, Berkeley Animal Use and Care Committee. C57BL/6, C57BL/6-Tg(Ins2-TFRC/OVA)296Wehi/WehiJ (RIPmOVA), B6(Cg)-Rag2tm1.1Cgn/J (Rag2^−/−), B6.C-H2-Kbm1/ByJ (Kbm1), B6.129P2-Il2tm1Hor/J (IL-2^−/−), and B6.SJL-Ptprca Pepcb/BoyJ (CD45.1) were from Jackson Labs. OT-I Rag2^−/− mice were from Taconic Farms. OT-I Rag2^−/− CD45.1 mice were generated by crossing OT-I Rag2^−/− mice to CD45.1 mice. F5 Rag1^−/− mice⁵⁴ , CD11cYFP mice^{55 (link)}, and AireGFP mice^{56 (link)} have been previously described. CD11cYFP RIPmOVA mice and AireGFP RIPmOVA mice were generated by crossing CD11cYFP or AireGFP mice to RIPmOVA mice, respectively. IL15Rα^−/−mice were generated by crossing B6.C-Tg(CMV-cre)1Cgn/J (Cmv-cre) mice with C57BL/6-Il15ratm2.1Ama/J (IL15R flox/flox) mice, both from Jackson Labs. To generate bone marrow chimeras, bone marrow was collected from the tibias and femurs of donor mice, then treated with ammonium chloride–potassium bicarbonate buffer to lyse red blood cells. Host mice were lethally irradiated (900rads) prior to intravenous injection of 5×10⁶ bone marrow cells.

Male C57BL/6J (B6; H2K^b), BALB/c (H2K^d), B6.FVB-Tg(Itgax-DTR/EGFP)57Lan/J (CD11c-DTR), and B6.129P2-IL2^tm1Hor/J (IL-2^−/−) mice were purchased from The Jackson Laboratory (Bar Harbor, ME). BALB/c St2^−/− mice were obtained from Dr. Andrew N.J. McKenzie and bred for experimental use^{25 (link)}. C57BL/6-Foxp3^tm1Flv/J (Foxp3-IRES-mRFP; FIR) reporter mice^{26 (link)} for breeding were graciously provided by Dr. Fadi Lakkis. All mice were housed in the specific pathogen-free facility at the University of Pittsburgh School of Medicine. Recombinant(r) mouse(m) IL-33 (BioLegend, San Diego, CA) was dissolved in PBS and injected intraperitoneally (0.5 μg/m/day(d); for 10 d). Control animals received PBS alone. Experiments were conducted under an Institutional Animal Care and Use Committee-approved protocol and in accordance with National Institutes of Health guidelines. Animals were fed a diet of Purina rodent chow (Ralston Purina, St. Louis, MO) and received food and water ad libitum.

All mice were bred and maintained under pathogen-free conditions within American Association of Laboratory Animal Care approved facilities at the University of California, Berkeley. The internal review board and the Animal Use and Care Committee at UC Berkeley approved all procedures. C57BL/6, B6.SJL-Ptprca Pepcb/BoyJ (CD45.1), 2D2 TCR transgenic, AND TCR transgenic, B6.129P2-Il2tm1Hor/J(IL2−/−), C57BL/6-Tg(Ins2-TFRC/OVA)296Wehi/WehiJ (RIPmOVA), and Foxp3-GFP mice were purchased from Jackson. 2D2×Rag2^−/− mice were generated by crossing 2D2 mice with Rag2^−/− mice purchased from Taconic. OT-II×Rag2^−/− TCR transgenic mice were purchased from Taconic. F5×Rag2^−/− mice have been described previously^{45 (link)}. Endotoxin-free soluble ovalbumin was purchased from Invivogen, and injections were performed via i.v. injection, followed by sacrifice 1 h later.