Recombinant human sdf 1α cxcl12

The reagents for electrospinning, PCL (Mw =80 kDa), dichloromethane and dimethyl sulfoxide (DMSO), were purchased from Sigma-Aldrich (USA). The reagents for LbL self-assembly, polyallylamine hydrochloride (PAH), CS (Mw=50-190 kDa) and HA (Mw=1500-1800 kDa) were also obtained from Sigma-Aldrich (USA). Dulbecco’s modified Eagle’s medium (DMEM), PBS, Fetal bovine serum (FBS), penicillin–streptomycin and trypsin were purchased from Gibco (USA). Recombinant human BMP-2 (rhBMP-2) and recombinant human SDF-1α/CXCL12 were purchased from Peprotech (USA).

Human PDGF-BB protein was purchased from Novus Biologicals (cat. no. NBP2-35203), LLC. Recombinant human SDF-1α (CXCL12) was obtained from PeproTech (cat. no. 300-28A), Inc. AMD3100 (a CXCR4 inhibitor, cat. no. S8030) and niclosamide (a STAT3 inhibitor, cat. no. S3030) were purchased from Selleck Chemicals. Anti-CXCR4 (cat. no. ab124824, 1:100), anti-CXCR7 (cat. no. ab72100, at 6 µg/ml), and anti-PDGFR-β (cat. no. ab69506, at 1 µg/ml) were obtained from Abcam. Anti-β-tubulin was obtained from Absin Biotechnology Co., Ltd (cat. no. abs830032, at 0.5 µg/ml). Anti-STAT3 (cat. no. ET1605-45, 1:1,000) and anti-phosphorylated (p)-STAT3 (cat. no. ET1603-40, 1:1,000) were obtained from Hangzhou Hua'An Biotechnology Co., Ltd. Anti-ERK-1/2 (cat. no. 9102, 1:1,000), anti-p-ERK1/2 (cat. no. 9106, 1:2,000), anti-AKT (cat. no. 9272, 1:1,000), and anti-p-AKT (cat. no. 9611, 1:1,000) were obtained from Cell Signaling Technology, Inc. Anti-mouse horseradish peroxidase (HRP)-conjugated secondary antibodies (cat. no. A0216, 1:1,000) and anti-rabbit HRP-conjugated secondary antibodies (cat. no. A0208, 1:1,000) were obtained from Beyotime Institute of Biotechnology. Primary Antibody Dilution Buffer (cat. no. P0023A) and Secondary Antibody Dilution Buffer (cat. no. P0023D) were also obtained from Beyotime Institute of Biotechnology.