Morusinol (purity >98%; determined by high-performance liquid chromatography), 3-(4, 5-dimethyl-2-thiazolyl)-2, and 5-diphenyl-2H-tetrazolium bromide (MTT) were obtained from SigmaAldrich Chemical Co. (St. Louis, MO, USA). Propidium iodide was purchased from Wuhan Boster Biological Technology (Wuhan, China). Dulbecco’s modified Eagle’s medium (DMEM) was purchased from HyClone (Logan, UT, USA). Fetal bovine serum (FBS), penicillin, and streptomycin were purchased from Tianjin HaoYang Biological Manufacture Co. (Tianjin, China). Horseradish peroxidase-labelled anti-mouse and anti-rabbit secondary antibodies and all other antibodies were purchased from Cell Signalling Technology (MA, USA). Cell culture plasticware was purchased from BD Biosciences (San Jose, CA, USA).
Propidium iodide
Manufactured by Wuhan Boster Biological Technology
Sourced in China
Propidium iodide is a fluorescent dye used in various biological applications. It binds to DNA, emitting red fluorescence upon excitation, and is commonly used to stain and visualize cellular nuclei.
Automatically generated - may contain errors
Lab products found in correlation
Cell culture plasticware, by BD (4 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (3 mentions)
Annexin 5 fitc, by Wuhan Boster Biological Technology (3 mentions)
3 4 5 dimethyl 2 thiazolyl 2, by Merck Group (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thomas Scientific (2 mentions)
Streptomycin, by Thomas Scientific (2 mentions)
Heat inactivated fetal calf serum, by Thomas Scientific (2 mentions)
Acridine orange, by Wuhan Boster Biological Technology (2 mentions)
Horseradish peroxidase labeled anti mouse and anti rabbit secondary antibodies, by Cell Signaling Technology (2 mentions)
5 methoxypsoralen, by Merck Group (1 mentions)
Hesperidin, by Merck Group (1 mentions)
3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide, by Merck Group (1 mentions)
Asiaticoside, by Merck Group (1 mentions)
6 protocols using propidium iodide
1
Morusinol-Induced Cytotoxicity Assay
2
Cytotoxicity Assay Protocol
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5-Methoxypsoralen (≥ 95% by HPLC), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), and dimethyl sulfoxide were obtained from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Acridine orange and propidium iodide were procured from Wuhan Boster Biological Technology Ltd. (Wuhan, China). Dulbecco’s modified Eagle’s medium and RPMI-1640 medium were obtained from Gibco Life Technologies (Grand Island, NY, USA). Heat-inactivated fetal calf serum, penicillin, and streptomycin were obtained from Thomas Scientific, High Hill Road, Swedesboro, U.S.A.
3
Hesperidin Cell Viability and Apoptosis Assay
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Hesperidin (purity >98%; determined by high-performance liquid chromatography) and MTT were obtained from Sigma-Aldrich; Merck KGaA (Darmstadt, Germany). Annexin V-FITC and propidium iodide were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China). Dulbecco's modified Eagle's medium (DMEM) and RPMI-1640 medium were purchased from HyClone (GE Healthcare, Chicago, IL, USA). Fetal bovine serum (FBS), penicillin and streptomycin were purchased from Tianjin HaoYang Biological Manufacture Co., Ltd. (Tianjin, China). Horseradish peroxidase-labeled anti-mouse and anti-rabbit secondary antibodies and all other antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). Cell culture plastic ware was purchased from BD Biosciences (San Jose, CA, USA).
4
Asiaticoside Induced Apoptosis Analysis
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Asiaticoside (purity >98%, determined by high-performance liquid chromatography), 3-(4, 5-dimethyl-2-thiazolyl) -2, 5-diphenyl-2H-tetrazolium bromide (MTT) were obtained from SigmaAldrich Chemical Co. (St. Louis, MO, USA). Annexin V-FITC and propidium iodide were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China). Dulbecco’s modified Eagle’s medium (DMEM) and RPMI-1640 medium were purchased from HyClone (Logan, UT, USA). Fetal bovine serum (FBS), penicillin, and streptomycin were purchased from Tianjin HaoYang Biological Manufacture Co., Ltd. (Tianjin, China). Horseradish peroxidase-labeled anti-mice and anti-rabbit secondary antibodies and all other antibodies were purchased from Cell Signaling Technology (MA, USA). Cell culture plasticware was purchased from BD Biosciences (San Jose, CA, USA).
5
Cytotoxicity Evaluation of Methylwogonin
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Methylwogonin (≥ 95%), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), and dimethyl sulfoxide were obtained from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Acridine orange, propidium iodide and Hoechst 33342 were procured from Wuhan Boster Biological Technology Ltd. (Wuhan, China). Dulbecco’s modified Eagle’s medium and RPMI-1640 medium were obtained from Gibco Life Technologies, Grand Island, NY, USA). Heat-inactivated fetal calf serum, penicillin, and streptomycin were obtained from Thomas Scientific, High Hill Road, Swedesboro, U.S.A.
6
Glychionide-A Cytotoxicity Evaluation
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Glychionide-A (purity >98%; determined by high-performance liquid chromatography), 3-(4, 5-dimethyl-2-thiazolyl)-2, and 5-diphenyl-2H-tetrazolium bromide (MTT) were obtained from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Annexin V-FITC and propidium iodide were purchased from Wuhan Boster Biological Technology (Wuhan, China). Dulbecco’s modified Eagle’s medium (DMEM) and RPMI-1640 medium were purchased from HyClone (Logan, UT, USA). Fetal bovine serum (FBS), penicillin, and streptomycin were purchased from Tianjin HaoYang Biological Manufacture Co. (Tianjin, China). Horseradish peroxidase-labeled anti-mouse and anti-rabbit secondary antibodies and all other antibodies were purchased from Cell Signalling Technology (MA, USA). Cell culture plasticware was purchased from BD Biosciences (San Jose, CA, USA).
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