Helos

Manufactured by Sympatec

Sourced in Germany

The HELOS is a laser diffraction particle size analyzer developed by Sympatec. It measures the size distribution of particles in a sample by analyzing the patterns of light scattered by the particles when a laser beam passes through them. The HELOS provides precise and reliable particle size data for a wide range of materials and applications.

Automatically generated - may contain errors

Lab products found in correlation

Up400, by Hielscher (2 mentions) Rodos, by Sympatec (2 mentions) Rodos m disperser, by Sympatec (1 mentions) Aspiros sample feeder, by Sympatec (1 mentions) Windox 5, by Sympatec (1 mentions) Accupyc 1330, by Micromeritics (1 mentions) Jsm it100, by JEOL (1 mentions) Ultra 55, by Zeiss (1 mentions) D8 advance, by Bruker (1 mentions) Leo 1550, by Zeiss (1 mentions) Sucell, by Sympatec (1 mentions) Ultrapyc 1200e, by Anton Paar (1 mentions) Christ alpha 1 2 ld plus, by Martin Christ (1 mentions) Heraeus megafuge 40r, by Thermo Fisher Scientific (1 mentions)

21 protocols using helos

Particle Size Distribution of Oleogels

Check if the same lab product or an alternative is used in the 5 most similar protocols

The particle size distribution of the oleogels was measured through laser diffraction using a Sympatec Helos (Sympatec GmbH, Clausthal-Zellerfeld, Germany), with a lens focal length of 100 mm and measuring range of 0.9–175 µm. For these measurements oleogels were produced with light liquid paraffin and an extract concentration of 8%. After diluting the sample in a ratio of 1:10 with the oil phase, the sample was added in a cuvette where it was stirred in paraffin to get a homogeneous dispersion. Measurements were taken at an optical concentration of 25–30%.
Laser diffraction results are expressed as ×₁₀, ×₅₀, and ×₉₀ values based on a cumulative volume distribution. To ensure comparability between rheology and particle size measurements, respective oleogels for particle sizing were also stored at 25 °C.

Ghaffar K.A, & Daniels R. (2020). Oleogels with Birch Bark Dry Extract: Extract Saving Formulations through Gelation Enhancing Additives. Pharmaceutics, 12(2), 184.

+ Open protocol

+ Expand

Particle Size Analysis of Powder Formulations

Check if the same lab product or an alternative is used in the 5 most similar protocols

The primary particle volumetric diameter (Dv) of each formulation was determined by laser diffraction using the Sympatec HELOS (submicron R1 lens with 20 mm focal length) equipped with RODOS/M disperser and ASPIROS sample feeder (Sympatec GmbH, Germany). Powder formulations were dispersed at a pressure of 4 bar. The span was calculated and reported for each measurement using the following equation

Span = (D v 90 - D v 10) / D v 50.

Hassan A., Farkas D., Longest W, & Hindle M. (2020). Characterization of excipient enhanced growth (EEG) tobramycin dry powder aerosol formulations. International journal of pharmaceutics, 591, 120027.

+ Open protocol

+ Expand

Particle Size Measurement by Laser Diffraction

Check if the same lab product or an alternative is used in the 5 most similar protocols

The particle size was measured by wet-sizing using Sympatec HELOS and CUVETTE laser diffraction system (Sympatec GmbH, Clausthal-Zellerfeld, Germany) with a R3 lens (0.9–175 μm).

Zadsirjan S., Dehkordi N.P., Heidari S., Najafi F., Zargar N., Feli M, & Salimnezhad S. (2024). Synthesis of a Calcium Silicate Cement Containing a Calcinated Strontium Silicate Phase. International Journal of Dentistry, 2024, 8875014.

+ Open protocol

+ Expand

Particle Size Analysis by Laser Diffraction

Check if the same lab product or an alternative is used in the 5 most similar protocols

The particle size distribution of the materials was determined by laser diffractometry using a Sympatec HELOS laser diffraction instrument (Sympatec GmbH, Clausthal-Zellerfield, Germany). To disperse the materials before measurement, a dispersion pressure of 4 Bar was used. The carrier was analysed using the R5 lens (0.5 to 875 μm) and the APIs was analysed with the R1 lens (0.3 to 80 μm). The particle diameter (D) was derived using Fraunhofer theory and D₁₀, D₅₀, D₉₀ were determined from a cumulative volume distribution. The span of the distribution was calculated as reported earlier (Rudén et al. 2018 (link)). The reported values in Table 1 are the mean and standard deviation from three measurements.

Rudén J., Frenning G., Bramer T., Thalberg K, & Alderborn G. (2020). On the relationship between blend state and dispersibility of adhesive mixtures containing active pharmaceutical ingredients. International Journal of Pharmaceutics: X, 3, 100069.

+ Open protocol

+ Expand

Dry Powder Aerodynamic Performance

Check if the same lab product or an alternative is used in the 5 most similar protocols

The dry powder was analyzed by laser diffraction (Helos, Sympatec GmbH, Clausthal-Zellerfeld, Germany) upon dry dispersion at 3 bar. Further, dispersion and aerodynamic performance were tested with the Cyclohaler^®, a dry powder inhaler device (DPI). For this, 20 mg of powder was weighed individually into HPMC capsules. The capsule was pierced in the device and dispersed into the Next Generation Pharmaceutical Impactor at an airflow of 100 L/min for 2.4 s equalling 4 L, according to Ph.Eur. 2.9.18. The resulting fine particle fraction FPF_emitted represents the percentage of particles <5 µm of the emitted dose as measured by NGI (n = 3).

Ebensen T., Arntz A., Schulze K., Hanefeld A., Guzmán C.A, & Scherließ R. (2023). Pulmonary Application of Novel Antigen-Loaded Chitosan Nano-Particles Co-Administered with the Mucosal Adjuvant C-Di-AMP Resulted in Enhanced Immune Stimulation and Dose Sparing Capacity. Pharmaceutics, 15(4), 1238.

+ Open protocol

+ Expand

Particle Size Measurement of PW Supernatant

Check if the same lab product or an alternative is used in the 5 most similar protocols

A laser diffraction system HELOS (Sympatec, Germany) with the RHODOS dispersing unit was used to measure the particle size distribution over a wide range of sizes of the PW supernatant.

Reppke M.J., Gerstner R., Windeisen-Holzhauser E., Richter K, & Benz J.P. (2022). Press water from the mechanical drying of Douglas-fir wood chips has multiple beneficial effects on lignocellulolytic fungi. Fungal Biology and Biotechnology, 9, 10.

+ Open protocol

+ Expand

Characterization of NMC-Based LIB Battery Waste

Check if the same lab product or an alternative is used in the 5 most similar protocols

A partner company provided pre-treated BM from spent NMC-based LIBs. The pre-treatment process involved discharging, dismantling, thermal treatment, crushing, and sorting. Inductively coupled plasma mass spectrometry (ICP-MS) was used to determine the elemental composition of the BM (Table 1), after acid digestion of the BM using aqua regia (according to ÖNORM EN 13657:2002-12). The particle size of the BM sample was determined according to ISO 13320-1, using a HELOS (Sympatec GmbH, Germany) particle size distribution measurement device, resulting in d₁₀ = 4.4 μm, d₅₀ = 17.2 μm, and d₉₀ = 55.0 μm.

Lalropuia L., Kucera J., Rassy W.Y., Pakostova E., Schild D., Mandl M., Kremser K, & Guebitz G.M. (2024). Metal recovery from spent lithium-ion batteries via two-step bioleaching using adapted chemolithotrophs from an acidic mine pit lake. Frontiers in Microbiology, 15, 1347072.

+ Open protocol

+ Expand

Particle Size Characterization of Excipients

Check if the same lab product or an alternative is used in the 5 most similar protocols

Laser diffraction measurements provided particle size distributions (PSD) of all used excipients. We used the Helium Laser Optical System (HELOS^®, Sympatec, Clausthal-Zellerfeld, Germany) equipped with the dry dispersion unit (RODOS^®) and an automatic feeder (VIBRI^®). The samples were dispersed by pressurised air (primary dispersion pressure of 2 bar for carrier samples and 3 bar for extrinsic fines). Depending on the sample, we used either an R1, R3 or an R4 lens. All data are shown as average of triplicate measurements. The respective standard deviation is depicted accordingly.

Bungert N., Kobler M, & Scherließ R. (2022). Proof-of-Concept for Adjusted Surface Energies and Modified Fines as a Novel Concept in Particle Engineering for DPI Formulations. Pharmaceutics, 14(5), 951.

+ Open protocol

+ Expand

Particle Size Distribution Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Laser diffraction was applied to determine the particle size distribution (PSD) of the initial and processed material. For this, a Helium Neon Laser Optical System (HELOS^®, Sympatec GmbH, Clausthal-Zellerfeld, Germany) was equipped with a RODOS^® dispersion module and a R4 lens with a measuring range of 0.5 to 350 µm. With compressed air (2 bar), the automatically fed (VIBRI^® dry dosing system, Sympatec GmbH) material was dispersed in an aerosol jet towards the measuring zone.
Evaluation of the size distribution was performed with the Windox 5 software (Sympatec GmbH) based on the Fraunhofer enhanced equation.

Bungert N., Kobler M, & Scherließ R. (2021). In-Depth Comparison of Dry Particle Coating Processes Used in DPI Particle Engineering. Pharmaceutics, 13(4), 580.

+ Open protocol

+ Expand

Dexamethasone Nanoparticle Formulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 6

A solution of 10 mg of dexamethasone in absolute ethanol was slowly dripped into 10 ml of F6H8 under stirring and at room temperature. Subsequently, the mixture was cooled with ice and ultrasonicated for 40 seconds (Hielscher, UP400S, 100% amplitude). Next, the ethanol was evaporated under vacuum using a rotary evaporator. A fine suspension was obtained whose particle size distribution was determined by laser diffraction (HELOS, Sympatec GmbH). In result, the volume mean diameter (VMD) was 12.20±0.17 m; the corresponding percentile diameters indicating the dimensions below which lie the diameters of 10%, 50% and 90% of the particles, were 2.52±0.03 μm (X₁₀), 10.28±0.11 μm (X₅₀) and 24.35±0.38 μm (X₉₀).

US11160865B2. Liquid pharmaceutical composition for the delivery of active ingredients (2021-11-02). None [DE], None [DE], None [DE]. Inventors: Bastian Theisinger [DE], Sonja Theisinger [DE], Bernhard Günther [DE].

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!