Gibco dmem high glucose medium

T47D cells (originally purchased from ATCC) were maintained in Gibco DMEM high glucose medium (Thermo) supplemented with 10% FBS (Atlanta). GATA3 mutant genes were generated by site-directed mutagenesis using the primers (Supplementary Figure S1A) and cloned into pHAGE lentiviral vectors (a kind gift from Dr. Guang Hu at NIEHS/NIH). The viruses were produced by transient transfection using 293T cells, psPAX2, and pMD2.G packaging vectors (psPAX2 and pMD2.G were gifts from Dr. Didier Trono, Addgene plasmid 12260, 12259). After infection, with lentiviruses encoding each GATA3 mutant, the stable cell pools were established by puromycin selection for 1 week. T47D cells infected with the lentivirus containing the pHAGE empty vector were used as a control cell line.

T47D cells were resuspended in Gibco DMEM high glucose medium (Thermo) containing 10% FBS (Atlanta). Fifty thousand cells per well were plated in a 24-well plate. At Days 1, 3, and 5, cells were harvested and then counted by DeNovix CellDrop.

The GBM cell lines U87, U251, and U251 EGFRvIII, which have been used in our previous publications (Chen et al., 2021 (link)), were grown in Gibco DMEM, high glucose medium (Thermo Fisher Scientific, 11965092) supplemented with 100 units of penicillin per mL plus 100 µg of streptomycin per mL (Life Technologies, 15140–122) and 5% fetal bovine serum, in a humidified 5% CO₂, 37°C incubator. U87 and U251 cell lines were authenticated by Labcorp.